AB MT CHEMISTRY EXAM 2026 CLINICAL
CHEMISTRY ASSESSMENT STUDY GUIDE
COMPLETE QUESTIONS AND ANSWERS
◉ When performing spectrophotometer quality control checks, the
holmium oxide glass filter is used to assess: Answer: Wavelength
accuracy
◉ Which type of elution technique may be used in high-performance
liquid chromatography? Answer: Gradient
◉ Which of the following may be associated with fluorescence
polarization? Answer: Plane-polarized light used for sample
excitation
◉ Which of the following may be associated with reflectance
spectrophotometry as it relates to the dry reagent slide technique?
Answer: Doesn't follow Beer's law, mathematical algorithms
necessary to linearize
◉ Which of the following may be associated with the colloid osmotic
pressure (COP) osmometer? Answer: Negative pressure on reference
(saline solution) side equivalent to COP of sample
,◉ Fluorometers are designed so that the path of the exciting light is
at a right angle to the path of the emitted light. The purpose of this
design is to: Answer: Prevent excitation light from reaching the
detector
◉ A chloride analyzer that generates silver ions as part of its
reaction mechanism operates on the principles of: Answer:
Amperometry and coulometry
◉ Interference in the quantitation of chloride by means of a
coulometric—amperometric system may be caused by the presence
in the test solution of: Answer: Bromide ions
◉ The measurement of CO2 in blood by means of a Pco2 electrode is
dependent on the: Answer: Change in pH due to increased carbonic
acid in the electrolyte surrounding the electrodes
◉ The measurement of oxygen in blood by means of a Po2 electrode
involves which of the following? Answer: Direct relationship
between amount of oxygen in the sample and amount of current
flowing in the measuring system
◉ Problems inherent in turbidimetry include all of the following
except: Answer: Need to maintain a very specific and constant high
temperature.
,◉ Which of the following statements best describes discrete
analysis? Answer: Each sample handled separately in its own
reaction vessel
◉ Which of the following statements applies to a double-beam-in-
time spectrophotometer? Answer: Has one chopper (has 2 detectors
& has 2 light sources----WRONG)
◉ Many chemical reactions require that an instrument maintain a
specified incubation temperature. How frequently should the
accuracy of the thermometer used to monitor the incubation
temperature be verified? Answer: Biannually
◉ Which of the following chromatography systems may be
described as having a stationary phase that is liquid adsorbed on
particles packed in a column and a liquid moving phase that is
pumped through a column? Answer: High-performance liquid
◉ All of the following apply to gas-liquid chromatography except:
Answer: Mobile phase is a liquid pumped through the column
(Separation depends on the sample's solubility in the liquid layer of
the stationary phase & Stationary phase is a liquid layer adsorbed on
the column packing----- WRONG)
, ◉ An electrophoretic technique employing a pH gradient that
separates molecules with similar isoelectric points is: Answer:
Isoelectric focusing
◉ Calculate the coefficient of variation (percent) for a set of data
where the mean = 89 mg/dL and 2 standard deviations (SD) = 14
mg/dL. Answer: 7.9
◉ The preparation of a Levey-Jennings quality control chart for any
single constituent of serum requires: Answer: 20 to 30 analyses of
control serum over a period of 20 different days
◉ A new standard has been prepared at a lower concentration than
that required for the test. If this is not known by the technologist,
and he/she assumes it is of the correct concentration, such an error
would appear on a quality control chart as a(an): Answer: Upward
Shift
◉ If the ±2 SD range of acceptable values for a digoxin control is
established as 2.0 to 2.6 ng/mL on the average, the expectation that
a value will be greater than 2.6 ng/mL is 1 in:
a. 10
b. 20
c. 40
d. 66
CHEMISTRY ASSESSMENT STUDY GUIDE
COMPLETE QUESTIONS AND ANSWERS
◉ When performing spectrophotometer quality control checks, the
holmium oxide glass filter is used to assess: Answer: Wavelength
accuracy
◉ Which type of elution technique may be used in high-performance
liquid chromatography? Answer: Gradient
◉ Which of the following may be associated with fluorescence
polarization? Answer: Plane-polarized light used for sample
excitation
◉ Which of the following may be associated with reflectance
spectrophotometry as it relates to the dry reagent slide technique?
Answer: Doesn't follow Beer's law, mathematical algorithms
necessary to linearize
◉ Which of the following may be associated with the colloid osmotic
pressure (COP) osmometer? Answer: Negative pressure on reference
(saline solution) side equivalent to COP of sample
,◉ Fluorometers are designed so that the path of the exciting light is
at a right angle to the path of the emitted light. The purpose of this
design is to: Answer: Prevent excitation light from reaching the
detector
◉ A chloride analyzer that generates silver ions as part of its
reaction mechanism operates on the principles of: Answer:
Amperometry and coulometry
◉ Interference in the quantitation of chloride by means of a
coulometric—amperometric system may be caused by the presence
in the test solution of: Answer: Bromide ions
◉ The measurement of CO2 in blood by means of a Pco2 electrode is
dependent on the: Answer: Change in pH due to increased carbonic
acid in the electrolyte surrounding the electrodes
◉ The measurement of oxygen in blood by means of a Po2 electrode
involves which of the following? Answer: Direct relationship
between amount of oxygen in the sample and amount of current
flowing in the measuring system
◉ Problems inherent in turbidimetry include all of the following
except: Answer: Need to maintain a very specific and constant high
temperature.
,◉ Which of the following statements best describes discrete
analysis? Answer: Each sample handled separately in its own
reaction vessel
◉ Which of the following statements applies to a double-beam-in-
time spectrophotometer? Answer: Has one chopper (has 2 detectors
& has 2 light sources----WRONG)
◉ Many chemical reactions require that an instrument maintain a
specified incubation temperature. How frequently should the
accuracy of the thermometer used to monitor the incubation
temperature be verified? Answer: Biannually
◉ Which of the following chromatography systems may be
described as having a stationary phase that is liquid adsorbed on
particles packed in a column and a liquid moving phase that is
pumped through a column? Answer: High-performance liquid
◉ All of the following apply to gas-liquid chromatography except:
Answer: Mobile phase is a liquid pumped through the column
(Separation depends on the sample's solubility in the liquid layer of
the stationary phase & Stationary phase is a liquid layer adsorbed on
the column packing----- WRONG)
, ◉ An electrophoretic technique employing a pH gradient that
separates molecules with similar isoelectric points is: Answer:
Isoelectric focusing
◉ Calculate the coefficient of variation (percent) for a set of data
where the mean = 89 mg/dL and 2 standard deviations (SD) = 14
mg/dL. Answer: 7.9
◉ The preparation of a Levey-Jennings quality control chart for any
single constituent of serum requires: Answer: 20 to 30 analyses of
control serum over a period of 20 different days
◉ A new standard has been prepared at a lower concentration than
that required for the test. If this is not known by the technologist,
and he/she assumes it is of the correct concentration, such an error
would appear on a quality control chart as a(an): Answer: Upward
Shift
◉ If the ±2 SD range of acceptable values for a digoxin control is
established as 2.0 to 2.6 ng/mL on the average, the expectation that
a value will be greater than 2.6 ng/mL is 1 in:
a. 10
b. 20
c. 40
d. 66
