TECHNOLOGY CHEMISTRY TEST PAPER
QUESTIONS AND SOLUTIONS GRADED A+
◉ In spectrophotometric analysis, what is the purpose of the reagent
blank? Answer: Correct for color contribution of the reagents
◉ Which of the following represents a primary advantage of
performing fluorometric over absorption spectroscopic methods of
analysis? Answer: Increased specificity and increased sensitivity
◉ In an electrolytic cell, which of the following is the half-cell where
reduction takes place? Answer: Cathode
◉ Which of the following is an advantage of the angle-head
centrifuge over the horizontal-head centrifuge? Answer: All of the
above
◉ In addition to utilizing Levey-Jennings charts, what other criteria
should be applied to interpret internal quality control data? Answer:
Westgard multirule
,◉ What is the purpose of a Youden plot? Answer: Compares results
on two control specimens, low and high controls, for the same
analyte analyzed by several laboratories
◉ Which Westgard multirule applies to a situation where one
control point exceeds the mean by +2 s and a second control point
exceeds the mean by -2 s ? Answer: R4s
◉ When comparing a potential new test with a comparative method
in order to bring a new method into the laboratory, one observes
error that is consistently affecting results in one direction. What is
this type of error known as? Answer: Systematic error
◉ A tech is scheduled to perform a specialized test that she/he is
familiar with, but is not exactly certain of the steps required. What is
the best course of action to take? Answer: Consult the procedure
manual and notify the supervisor.
◉ A tech has completed the first run of morning specimens. She/he
notices that the one control being used is outside +3 s. What course
of action should be taken? Answer: Check equipment and reagents
to determine source of error; repeat the entire analysis including the
control and patients; if the control value is within +2 s release
results.
,◉ A new test to assess for the presence of malignancy has been
developed. By testing a group of benign individuals, it is determined
that 45 of 50 subjects test negative for the new marker. What is the
specificity of this new assay? Answer: 90%
◉ Which of the following has as its purpose to promote the
incorporation of principles of quality management and quality
assurance into daily work routines? Answer: ISO 9000
◉ Mercury covered by a layer of mercurous chloride in contact with
saturated potassium chloride solution is a description of which of
the following types of electrodes? Answer: Calomel
◉ Colligative properties of solutions include all of the following
except: Answer: pH
◉ The freezing point osmometer is based on the principle that:
Answer: The freezing point is raised by an amount that is inversely
proportional to the concentration of dissolved particles in the
solution (wrong)
◉ In assaying an analyte with a single-beam atomic absorption
spectrophotometer, what the instrument actually measures is the:
Answer: Intensity of the beam from the hollow cathode lamp after it
has passed through the analyte containing flame
, ◉ Nephelometry is a method of assay based on the measurement of
the light that is: Answer: Scattered by particles in suspension
◉ To achieve the best levels of sensitivity and specificity, a type of
detector system that a gas chromatograph may be coupled to is
a(an): Answer: Mass spectrometer
◉ Given the following information, calculate the plasma osmolality
in milliosmoles per kilogram: sodium—142 mmol/L: glucose—120
mg/dL; urea nitrogen—18mg/dL. Answer: 296
◉ All the following are true of ion-selective electrode analysis of
sodium except: Answer: membrane coated with valinomycin
◉ When performing spectrophotometer quality control checks, the
holmium oxide glass filter is used to assess: Answer: Wavelength
accuracy
◉ Which type of elution technique may be used in high-performance
liquid chromatography? Answer: Gradient
◉ Which of the following may be associated with fluorescence
polarization? Answer: Plane-polarized light used for sample
excitation