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Microbiology Quiz Ch 2 Case studies

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Urinary Tract Infections Getting the Goods Read this case file and complete the activities that follow. As a nurse working in a busy obstetrics and gynecology practice, my job often included instructing pregnant women in collecting urine samples. Every expectant mother who attended the clinic provided a urine sample at every visit. A pregnant woman is at higher risk of developing urinary tract infections (UTIs) due to the increasing weight of her growing uterus, which compresses the bladder and prevents the bladder from draining completely. Urine left behind in the bladder becomes the perfect medium for bacterial growth. I instructed a young mother how to properly collect a midstream urine sample. I told the patient to first wash her hands. I emphasized that she should ensure that her hands did not come in contact with the rim of the collection container. I further instructed her on how to cleanse the external genitalia with a disposable wipe saturated with povidone-iodine, a potent antimicrobial solution. I reminded her to wipe from front to back to prevent fecal contamination. I told her she was to void a small amount of urine into the toilet, then introduce the collection container into the urine stream, collecting the midstream portion of the urine. She was instructed to put the lid on the collection container, being careful not to touch the rim or the inside of the lid, and then wash her hands. I then donned gloves, wiped the outside of the container and delivered the specimen to the lab, after labeling it with the patient’s name, the date and time of collection, and additional identification information. The laboratory staff examined a small amount of urine under the microscope for the presence of bacteria, red blood cells, white blood cells, and other abnormalities. The lab staff identified the presence of bacteria, and the urine was cultured to identify the microorganism and to test its antibiotic sensitivity. After 48 hours, the culture result came back stating that the sample was contaminated. I informed the patient’s physician, who asked that the patient return to provide another urine sample. Urine specimens are one of the few specimens collected by patients themselves and may become contaminated easily due to poor collection technique. Failure to wash hands, accidentally touching the rim or lid of the collection container, and failure to properly cleanse the external genitalia (in female patients) prior to specimen collection are some of the ways in which specimens may become contaminated. In this case, the patient returned to provide another sample. Instructions were provided again, and the patient was asked whether she understood what was required of her. This time the sample yielded only one species, Escherichia coli, a bacterium that is a common causative agent of urinary tract infections. The patient was treated with antibiotics for 10 days, and a repeat culture was negative for any microorganisms. Concept Check #1 1. When a urine sample reaches the laboratory, what is the first technique used to determine if a urinary tract infection exists?

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Microbiology Quiz Two Study Question - Ch. 2



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Urinary Tract Infections
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Getting the Goods

Read this case file and complete the activities that follow.

As a nurse working in a busy obstetrics and gynecology practice, my job often
included instructing pregnant women in collecting urine samples. Every
expectant mother who attended the clinic provided a urine sample at every
visit. A pregnant woman is at higher risk of developing urinary tract infections
(UTIs) due to the increasing weight of her growing uterus, which compresses
the bladder and prevents the bladder from draining completely. Urine left
behind in the bladder becomes the perfect medium for bacterial growth. I
instructed a young mother how to properly collect a midstream urine sample. I
told the patient to first wash her hands. I emphasized that she should ensure
that her hands did not come in contact with the rim of the collection container.
I further instructed her on how to cleanse the external genitalia with a
disposable wipe saturated with povidone-iodine, a potent antimicrobial
solution.

I reminded her to wipe from front to back to prevent fecal contamination. I told
her she was to void a small amount of urine into the toilet, then introduce the
collection container into the urine stream, collecting the midstream portion of
the urine. She was instructed to put the lid on the collection container, being
careful not to touch the rim or the inside of the lid, and then wash her hands. I
then donned gloves, wiped the outside of the container and delivered the
specimen to the lab, after labeling it with the patient’s name, the date and time
of collection, and additional identification information.

The laboratory staff examined a small amount of urine under the microscope
for the presence of bacteria, red blood cells, white blood cells, and other
abnormalities. The lab staff identified the presence of bacteria, and the urine
was cultured to identify the microorganism and to test its antibiotic sensitivity.
After 48 hours, the culture result came back stating that the sample was
contaminated. I informed the patient’s physician, who asked that the patient
return to provide another urine sample.

, Urine specimens are one of the few specimens collected by patients
themselves and may become contaminated easily due to poor collection
technique. Failure to wash hands, accidentally touching the rim or lid of the
collection container, and failure to properly cleanse the external genitalia (in
female patients) prior to specimen collection are some of the ways in which
specimens may become contaminated. In this case, the patient returned to
provide another sample. Instructions were provided again, and the patient was
asked whether she understood what was required of her. This time the
sample yielded only one species, Escherichia coli, a bacterium that is a
common causative agent of urinary tract infections. The patient was treated
with antibiotics for 10 days, and a repeat culture was negative for any
microorganisms.
Concept Check #1

1. When a urine sample reaches the laboratory, what is the first technique
used to determine if a urinary tract infection exists?
multiple choice 1
 Sample is allowed to sit for days to observe cloudiness
 Sample is observed microscopically Correct
 Sample is biochemically tested
 Sample is grown on an agar plate


2. It is normal to have a relatively large amount of bacteria in a clean catch
sample.
False Correct

3. In the case, the patient is treated with antibiotics for her UTI. This is
because Escherichia coli is a ______.
multiple choice 2
 prion
 bacterium Correct
 virus
 helminth


4. Why are urinary tract infections more common in women?
multiple choice 3
 They carry more fecal bacteria
 Only pregnant individuals can get UTIs
 Their vaginal tract is highly acidic

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