BIS 102 MIDTERM 3 QUESTIONS
BANK; COMPLETE ACCURATE QUIZZES
WITH VERIFIED ANSWERS
1 unit (u) of enzyme is - correct answer- the amount of
enzyme that can transform 1 umolS --> P/minute @25 *C
1. crude cellular extract - correct answer- fraction vol
(mL) = 1,400
total protein (mg) = 10,000
activity (units) = 100,000
specific activity (units/mg) = 10
2 types of membrane proteins (differ in structure and function)
- correct answer- integral membrane proteins and
peripheral membrane
2. precipitation with ammonium sulfate - correct answer-
fraction vol (mL) = 280
total protein (mg) = 3,000
activity (units) = 96,000
specific activity (units/mg) = 32
,2D gel electropores - correct answer- protein sample ->
separate proteins in first dimension on gel strip with isoelectric
focusing -> gel strip - pH9 (left) to pH3 (right) -> separate
proteins in second dimension on SDS-polyacrylamide gel
3. ion exchange chromatography - correct answer-
fraction vol (mL) = 90
total protein (mg) = 400
activity (units) = 80,000
specific activity (units/mg) = 200
4. size exclusion chromatography - correct answer-
fraction vol (mL) = 80
total protein (mg) = 100
activity (units) = 60,000
specific activity (units/mg) = 600
5. affinity chromatography - correct answer- fraction vol
(mL) = 6
total protein (mg) = 3
activity (units) = 45,000
, specific activity (units/mg) = 15,000
a chart on the composition of different lipid types in different
organelle membranes conclude - correct answer-
functional specialization of each type of membrane reflected in
its unique lipid composition
a protein sample may be applied to - correct answer-
one end of a gel strip with an immobilized pH gradient
or a protein sample in a solution of ampholytes may be used to
rehydrate a dehydrated gel strip
a pure sample of a protein - correct answer- gets rid of
any unwanted proteins
maximizes yield of enzyme
activity _____________ bc you lose some in every step -
correct answer- decreases
activity is - correct answer- the total units of enzyme in a
solution
BANK; COMPLETE ACCURATE QUIZZES
WITH VERIFIED ANSWERS
1 unit (u) of enzyme is - correct answer- the amount of
enzyme that can transform 1 umolS --> P/minute @25 *C
1. crude cellular extract - correct answer- fraction vol
(mL) = 1,400
total protein (mg) = 10,000
activity (units) = 100,000
specific activity (units/mg) = 10
2 types of membrane proteins (differ in structure and function)
- correct answer- integral membrane proteins and
peripheral membrane
2. precipitation with ammonium sulfate - correct answer-
fraction vol (mL) = 280
total protein (mg) = 3,000
activity (units) = 96,000
specific activity (units/mg) = 32
,2D gel electropores - correct answer- protein sample ->
separate proteins in first dimension on gel strip with isoelectric
focusing -> gel strip - pH9 (left) to pH3 (right) -> separate
proteins in second dimension on SDS-polyacrylamide gel
3. ion exchange chromatography - correct answer-
fraction vol (mL) = 90
total protein (mg) = 400
activity (units) = 80,000
specific activity (units/mg) = 200
4. size exclusion chromatography - correct answer-
fraction vol (mL) = 80
total protein (mg) = 100
activity (units) = 60,000
specific activity (units/mg) = 600
5. affinity chromatography - correct answer- fraction vol
(mL) = 6
total protein (mg) = 3
activity (units) = 45,000
, specific activity (units/mg) = 15,000
a chart on the composition of different lipid types in different
organelle membranes conclude - correct answer-
functional specialization of each type of membrane reflected in
its unique lipid composition
a protein sample may be applied to - correct answer-
one end of a gel strip with an immobilized pH gradient
or a protein sample in a solution of ampholytes may be used to
rehydrate a dehydrated gel strip
a pure sample of a protein - correct answer- gets rid of
any unwanted proteins
maximizes yield of enzyme
activity _____________ bc you lose some in every step -
correct answer- decreases
activity is - correct answer- the total units of enzyme in a
solution
