UBC CAPS 390 - Chapter 1 Questions and Answers.

UBC CAPS 390 - Chapter 1 Questions
and Answers.
Hematoxylin - Answer Blue: nucleus/nucleic acids, acidic regions of cytoplasm + cartilage
matrix



Eosin - Answer Pink: basic regions of cytoplasm + cartilage fibers



Masson's trichome - Answer Dark blue: nuclei

Red: muscle, keratin + cytoplasm

Light blue: mucinogen + collagen



Wiegert elastic stain - Answer Blue: elastic fibers



Silver strain - Answer Black: reticular fibers



Iron hematoxylin - Answer Black: muscle striations, nuclei + erythrocytes



Periodic acid-Schiff - Answer Magenta: glycogen + carbohydrate-rich molecules



Wright and Giemsa - Answer Pink: erythrocytes + eosinophil granules

Purple: leukocytes + basophil granules

Blue: cytoplasm of monocytes + lymphocytes



Mitochondria - Answer 0.2-3 um --> visible by both LM + EM



Individual cytoskeletal filaments - Answer 0.005-0.01 um --> visible by only EM



Trachea - Answer ~200 um --> visible by both LM + EM



Columnar cell - Answer ~20 um --> visible by both LM + EM



Nucleus - Answer ~3 um --> visible by both LM + EM



Cilia - Answer ~0.2 um --> visible by both LM + EM