QUIZ 1 - BIOL 1002 LAB - LABS 1 & 2 WITH
COMPLETE SOLUTIONS
Scientific Method - ANSWER -Observation
-Question and Hypothesis
-Experiment
-Results
-Conclusion
What is a hypothesis? - ANSWER A proposed explanation that suggests a
possible answer to specific questions
What is an independent variable? - ANSWER -The "manipulated" variable
-Experimental condition manipulated by the scientist
What is the dependent variable? - ANSWER -The "response" variable
-Measured in response to the experimental conditions or manipulations
What is the control variable? - ANSWER Held constant throughout the
experiment
What is the volume of micropipets? - ANSWER <1000 ul or 1 ml
What is the volume serological pipets? - ANSWER 1-10 ml
What is the volume of graduated cylinders? - ANSWER >10 ml
What is accuracy? - ANSWER A group of measurements referring to how close
the measured values agree with the true or correct value
What is precision? - ANSWER A group of measurements referring to how close
the measurements agree with each other
Solve this dilution problem:
Dilution = Vol(A)/Vol(A+B)
A = Solute
B = Solvent
A+B = Solution
--> A = 1 ml
--> B = 9 ml - ANSWER = 1(1+9) = 1/10 (a 1:10 dilution)
= 1/10 = 10^-1 (a 10-fold dilution)
, What is the objective of serial dilutions? - ANSWER To create a range of dilutions
of an organisms or component
Finish this:
1:10/10^-1 --> 1:100/10^-2 --> - ANSWER 1:1000/10^-3 --> 1:10,000/10^-4
Dilution factor (DF) = C1V1 = C2V2 - ANSWER -C1 = concentration of the stock
solution
-C2 = concentration of teh working solution (desired concentration)
-V1 = volume of the stock solution
-V2 = final volume of the working solution (desired volume)
Exampl Dilution Factor problem:
Make 500 ml of 3% CaCl from CaCl stock = 50% - ANSWER -> DF = C1V1=C2V2
-> DF = (50%)(V1) = (3%)(500ml)
-> DF = (50%)(V1)/(50%) = (3%)(500ml)/(50%)
-> DF = 30 ml
Dilute 30 ml of teh stock solution to 500 ml with dH2O
What are the four categories of macromolcules? - ANSWER 1. Carbohydrates
2. Proteins
3. Lipids
4. Nucleic Acids
What are macromolecules made out of? - ANSWER They are made up of small
subunits held together by covalent bonds
What sets macromolecules apart from one another? - ANSWER Each has a
different structure and properties
What is dehydration synthesis? - ANSWER links polymers by creating water as
bonds are formed
What is hydrolysis? - ANSWER Breaks bonds between polymers by returning a
molecule of water
What does Benedicts Test (Reducing Sugars) determine? - ANSWER It
determines whether a carbohydrate has a free aldehyde or ketone functional
group. The reducing sugar gives up electrons to the oxidizing agent Cu2+
forming cuprous oxide (Cu2O) = reddish brown to green precipitate
Crabohydrates:
-Monomer Form:
-Polymer Form:
-Formation:
-Bond:
COMPLETE SOLUTIONS
Scientific Method - ANSWER -Observation
-Question and Hypothesis
-Experiment
-Results
-Conclusion
What is a hypothesis? - ANSWER A proposed explanation that suggests a
possible answer to specific questions
What is an independent variable? - ANSWER -The "manipulated" variable
-Experimental condition manipulated by the scientist
What is the dependent variable? - ANSWER -The "response" variable
-Measured in response to the experimental conditions or manipulations
What is the control variable? - ANSWER Held constant throughout the
experiment
What is the volume of micropipets? - ANSWER <1000 ul or 1 ml
What is the volume serological pipets? - ANSWER 1-10 ml
What is the volume of graduated cylinders? - ANSWER >10 ml
What is accuracy? - ANSWER A group of measurements referring to how close
the measured values agree with the true or correct value
What is precision? - ANSWER A group of measurements referring to how close
the measurements agree with each other
Solve this dilution problem:
Dilution = Vol(A)/Vol(A+B)
A = Solute
B = Solvent
A+B = Solution
--> A = 1 ml
--> B = 9 ml - ANSWER = 1(1+9) = 1/10 (a 1:10 dilution)
= 1/10 = 10^-1 (a 10-fold dilution)
, What is the objective of serial dilutions? - ANSWER To create a range of dilutions
of an organisms or component
Finish this:
1:10/10^-1 --> 1:100/10^-2 --> - ANSWER 1:1000/10^-3 --> 1:10,000/10^-4
Dilution factor (DF) = C1V1 = C2V2 - ANSWER -C1 = concentration of the stock
solution
-C2 = concentration of teh working solution (desired concentration)
-V1 = volume of the stock solution
-V2 = final volume of the working solution (desired volume)
Exampl Dilution Factor problem:
Make 500 ml of 3% CaCl from CaCl stock = 50% - ANSWER -> DF = C1V1=C2V2
-> DF = (50%)(V1) = (3%)(500ml)
-> DF = (50%)(V1)/(50%) = (3%)(500ml)/(50%)
-> DF = 30 ml
Dilute 30 ml of teh stock solution to 500 ml with dH2O
What are the four categories of macromolcules? - ANSWER 1. Carbohydrates
2. Proteins
3. Lipids
4. Nucleic Acids
What are macromolecules made out of? - ANSWER They are made up of small
subunits held together by covalent bonds
What sets macromolecules apart from one another? - ANSWER Each has a
different structure and properties
What is dehydration synthesis? - ANSWER links polymers by creating water as
bonds are formed
What is hydrolysis? - ANSWER Breaks bonds between polymers by returning a
molecule of water
What does Benedicts Test (Reducing Sugars) determine? - ANSWER It
determines whether a carbohydrate has a free aldehyde or ketone functional
group. The reducing sugar gives up electrons to the oxidizing agent Cu2+
forming cuprous oxide (Cu2O) = reddish brown to green precipitate
Crabohydrates:
-Monomer Form:
-Polymer Form:
-Formation:
-Bond:
