MCB2010: quiz 2 study guide Daniel
Kulangara BC Broward College
Chapter 3 microbiology
Millimeter - Answer one thousandth of a meter
micrometer - Answer millionth of a meter
nanometer - Answer one billionth of a meter
compound microscope - Answer - can magnify up to 1.000x
- ocular and objective lenses
phase-contrast microscope - Answer - amplifies the difference between refractive index
(phase of light passing through specimens) of dense material and surrounding material.
- makes cells and other materials appear darker
fluorescent microscope - Answer - used to observe naturally fluorescent specimens, or
specimens that have been stained with a fluorescent dye.
- uses UV light projected ONTO specimen (not through).
- molecules absorb one wavelength and emit a light at a longer wavelength.
smear - Answer thin layer of specimen on a slide with liquid and coverslip
- allows to observe living organisms.
- can be difficult when specimen is colorless.
heat fixing - Answer makes specimen adhere to slide. can kill bacteria
chromophore - Answer gives the stain its color.
part of chromogen
auxochrome - Answer makes the dye charged either + or -
cationic stain - Answer positively charged stain
Kulangara BC Broward College
Chapter 3 microbiology
Millimeter - Answer one thousandth of a meter
micrometer - Answer millionth of a meter
nanometer - Answer one billionth of a meter
compound microscope - Answer - can magnify up to 1.000x
- ocular and objective lenses
phase-contrast microscope - Answer - amplifies the difference between refractive index
(phase of light passing through specimens) of dense material and surrounding material.
- makes cells and other materials appear darker
fluorescent microscope - Answer - used to observe naturally fluorescent specimens, or
specimens that have been stained with a fluorescent dye.
- uses UV light projected ONTO specimen (not through).
- molecules absorb one wavelength and emit a light at a longer wavelength.
smear - Answer thin layer of specimen on a slide with liquid and coverslip
- allows to observe living organisms.
- can be difficult when specimen is colorless.
heat fixing - Answer makes specimen adhere to slide. can kill bacteria
chromophore - Answer gives the stain its color.
part of chromogen
auxochrome - Answer makes the dye charged either + or -
cationic stain - Answer positively charged stain
