1|Page
DNA & Serology Final Verified Exam Study Set,
University of Florida Forensic Science Program,
2026/2027 -Question Examination with Verified
Solutions||Newest Exam!!!
What is the purpose of the phenol in the phenol:chloroform
organic extraction? - Answer-Solubalize polysaccharides
The Chelex resin is selective for______? - Answer-Heavy
metals and cations
Hairs that have been shed from the head (naturally fallen)
are of what category? - Answer-Telogen
What color blood tubes are preferred to use if DNA
extraction is necessary? - Answer-Purple
Generally chelex extraction is used in the recovery of DNA
from oral samples. However, due to the nature of one type
of sample organic extraction may be considered to provide
better or more reliable results. Which sample type is it? -
Answer-Chewing gum
,2|Page
True or False: The yield gel only measures total human
DNA. - Answer-False
Nucleic acids have UVVis absorption characteristics at
which wavelength range? - Answer-240-280 nm
True or False The slot blot method of DNA quantitation
can measure both double and single-stranded DNA? -
Answer-True
RT-PCR utilizes a modification of what? - Answer-
Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? -
Answer-Thermal cycler
True or False? Multiplex PCR uses more than one set of
PCR primers to target multiple locations. - Answer-True
True or False The target amount of extracted DNA
necessary for PCR is in the region of 0.5 2 µg. - Answer-
False
,3|Page
The short polynucleotide chains used in PCR to target
regions of amplification are called? - Answer-Primers
In what organism is Taq Polymerase found? - Answer-
Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? -
Answer-chelator
DNA fragments will travel - Answer-Toward the anode and
away from the cathode
How many STR loci can you get information about using
Profiler and Cofiler kits? - Answer-13
By using the Profiler and Cofiler Kits how
many amplifications does it take to obtain information on
all the CODIS loci? - Answer-2
Typical data applied to an electropherogram does NOT
include - Answer-Peak Area
, 4|Page
But it does include: name of locus, number of repeats, size
of fragment, and peak height.
What does the genescan software NOT do? - Answer-
Assigns allele calls to the peaks
Assuming two donors, consider one locus with alleles 12,
13 and 14. How many different ALLELIC combinations are
possible for one of the individuals in the mixture? -
Answer-6
True or False: You can analyze your data without an allelic
ladder? - Answer-False
What is a NOT a part of the ABI 310 Genetic Analyzer? -
Answer-Chiller
Parts include: Capillary, CCD camera, syringe with
polymer
DNA & Serology Final Verified Exam Study Set,
University of Florida Forensic Science Program,
2026/2027 -Question Examination with Verified
Solutions||Newest Exam!!!
What is the purpose of the phenol in the phenol:chloroform
organic extraction? - Answer-Solubalize polysaccharides
The Chelex resin is selective for______? - Answer-Heavy
metals and cations
Hairs that have been shed from the head (naturally fallen)
are of what category? - Answer-Telogen
What color blood tubes are preferred to use if DNA
extraction is necessary? - Answer-Purple
Generally chelex extraction is used in the recovery of DNA
from oral samples. However, due to the nature of one type
of sample organic extraction may be considered to provide
better or more reliable results. Which sample type is it? -
Answer-Chewing gum
,2|Page
True or False: The yield gel only measures total human
DNA. - Answer-False
Nucleic acids have UVVis absorption characteristics at
which wavelength range? - Answer-240-280 nm
True or False The slot blot method of DNA quantitation
can measure both double and single-stranded DNA? -
Answer-True
RT-PCR utilizes a modification of what? - Answer-
Polymerase Chain Reaction
What type of equipment is necessary to use for RT-PCR? -
Answer-Thermal cycler
True or False? Multiplex PCR uses more than one set of
PCR primers to target multiple locations. - Answer-True
True or False The target amount of extracted DNA
necessary for PCR is in the region of 0.5 2 µg. - Answer-
False
,3|Page
The short polynucleotide chains used in PCR to target
regions of amplification are called? - Answer-Primers
In what organism is Taq Polymerase found? - Answer-
Thermus Aquaticus
What is the purpose of EDTA during the PCR reaction? -
Answer-chelator
DNA fragments will travel - Answer-Toward the anode and
away from the cathode
How many STR loci can you get information about using
Profiler and Cofiler kits? - Answer-13
By using the Profiler and Cofiler Kits how
many amplifications does it take to obtain information on
all the CODIS loci? - Answer-2
Typical data applied to an electropherogram does NOT
include - Answer-Peak Area
, 4|Page
But it does include: name of locus, number of repeats, size
of fragment, and peak height.
What does the genescan software NOT do? - Answer-
Assigns allele calls to the peaks
Assuming two donors, consider one locus with alleles 12,
13 and 14. How many different ALLELIC combinations are
possible for one of the individuals in the mixture? -
Answer-6
True or False: You can analyze your data without an allelic
ladder? - Answer-False
What is a NOT a part of the ABI 310 Genetic Analyzer? -
Answer-Chiller
Parts include: Capillary, CCD camera, syringe with
polymer
