1. Which bacterium is most commonly associated with undercooked
ground beef?
A. Listeria monocytogenes
B. Salmonella Typhi
C. Escherichia coli O157:H7
D. Bacillus cereus
✓ Correct Answer: C
Rationale: E. coli O157:H7 is linked to undercooked ground beef; it
causes hemorrhagic colitis.
2. The infectious dose of Norovirus is approximately:
A. 10–100 viral particles
B. 1,000–10,000 viral particles
C. 100,000–1,000,000
D. 1 million+
✓ Correct Answer: A
Rationale: Norovirus is highly contagious; as few as 10–100 particles
can cause infection.
3. Which pathogen is capable of growth at refrigeration
temperatures (below 4°C/40°F)?
A. Clostridium botulinum
B. Staphylococcus aureus
C. Listeria monocytogenes
D. Salmonella spp.
✓ Correct Answer: C
Rationale: Listeria is psychrotrophic; it grows at refrigerated temps,
making ready-to-eat foods risky.
4. The most common viral cause of foodborne illness in the U.S. is:
A. Hepatitis A
B. Norovirus
C. Rotavirus
D. Astrovirus
,✓Correct Answer: B
Rationale: Norovirus causes over 50% of all foodborne outbreaks.
5. Which toxin is heat-stable and produced by Staphylococcus
aureus?
A. Botulinum toxin
B. Enterotoxin
C. Shiga toxin
D. Cytotoxin
✓ Correct Answer: B
Rationale: S. aureus enterotoxin is heat-stable; cooking does not
destroy it.
**6.Clostridium perfringens is often associated with:
A. Rice dishes
B. Raw oysters
C. Large roasts or gravy held at unsafe temps
D. Soft cheeses
✓ Correct Answer: C
Rationale: C. perfringens spores survive cooking; germinate during
slow cooling in bulk meats.
**7.Bacillus cereus emetic syndrome is linked primarily to:
A. Cooked rice
B. Deli meats
C. Unpasteurized milk
D. Canned vegetables
✓ Correct Answer: A
Rationale: Emetic toxin (cereulide) forms in starchy foods like fried
rice held at room temp.
**8.Vibrio vulnificus infection is associated with:
A. Undercooked chicken
B. Raw or undercooked shellfish
C. Contaminated lettuce
D. Unpasteurized juice
,✓Correct Answer: B
Rationale: Vibrio species are found in warm coastal waters; raw
oysters are a common vehicle.
**9.Clostridium botulinum spores are destroyed by:
A. Boiling for 10 minutes
B. Freezing
C. Acidic pH below 4.6
D. Commercial canning (121°C/250°F under pressure)
✓ Correct Answer: D
Rationale: Botulism spores require moist heat at 121°C (250°F) for 3
min (e.g., pressure canning).
10. Which parasite is associated with raw or undercooked
freshwater fish?
A. Trichinella spiralis
B. Taenia saginata
C. Anisakis simplex
D. Cryptosporidium parvum
✓ Correct Answer: C
Rationale: Anisakis is a nematode found in marine/freshwater fish;
causes anisakiasis.
**11.Campylobacter jejuni is commonly found in:
A. Raw poultry
B. Canned corn
C. Powdered milk
D. Acidic fruits
✓ Correct Answer: A
Rationale: Campylobacter colonizes poultry; cross-contamination is
a major risk.
12. The infectious dose of Shigella is:
A. Very low (10–100 cells)
B. Moderate (1,000–10,000)
C. High (100,000+)
D. Variable by strain
, ✓Correct Answer: A
Rationale: Shigellosis requires as few as 10–200 organisms due to
acid resistance.
13. Which virus requires a month-long incubation before jaundice
appears?
A. Norovirus
B. Hepatitis E
C. Hepatitis A
D. Rotavirus
✓ Correct Answer: C
Rationale: Hepatitis A average incubation 28 days (range 15–50
days).
14. The D-value of a microbe at 70°C is:
A. Time to kill 90% of population at 70°C
B. Temperature to kill 100% in 1 min
C. Decimal reduction time at 70°C
D. Both A and C
✓ Correct Answer: D
Rationale: D-value = time at a given temp to reduce population by 1
log (90%).
15. Which pathogen is NOT typically transmitted by fecal-oral route?
A. Norovirus
B. Listeria monocytogenes
C. Hepatitis A
D. Shigella
✓ Correct Answer: B
Rationale: Listeria is transmitted via contaminated RTE foods, soil, or
unpasteurized dairy, not primarily fecal-oral.
**16.Yersinia enterocolitica is associated with:
A. Raw pork
B. Canned tuna
C. Sprouts
D. Chocolate milk