PORTAGE MICROBIOLOGY FINAL CETIFIED QUALITY EXAM
QUESTIONS WITH DETAILED ANSWERS GUARANTEED PASS
Describe the ONE main differences between lytic and
temperate phages.
Lytic bacteriophages replicate within the host bacteria until it
ruptures, whereas temperate (or lysogenic) phages primarily
exist in a non-replicative state that does not kill the host cell.
Describe TWO main characteristics of LYTIC bacteriophage
replication
1. It replicates in the host cell until the host ruptures
2. It replicates all proteins that it needs to assemble new virus
particles
Bacteria that have a curved rod morphology are referred to as
______.
Vibrio
You want to observe the movement of microorganisms that
are present in a sample of cheek cells obtained on a patient.
Briefly explain the technique that you would use and how it is
performed.
,A wet mount stain is indicated. A small liquid culture (usually
just a drop) containing a microorganism of interest is
prepared, added to a slide, and then covered with a glass
coverslip. The coverslip is present to both protect the objective
and the specimen while also holding the microorganism in
place. Wet mounts are used to observe the motility and
behavior of an organism.
Briefly explain why a researcher would use the simple stain
technique. How is it preformed and what does it identify
It uses a solution of positively charged dye, which binds to and
stains the negatively changed membrane of the
microorganism.
This technique can show size, shape, and arrangement of cells
What coronavirus-associated infection gave rise to
quarantining and travel restrictions in 2003?
May also ask about 2020
2003: SARS
2020: Covid-19
Match each type of agar plate to the corresponding general
, Characteristics of each.
LB Agar
Trypticase Soy Agar
Macconkey Agar
Mannitol Salt Agar
LB Agar - Also known as lysogeny broth, it is a multi-purpose
media capable of growing a wide variety of microorganisms
Trypticase Soy Agar - A multi-purpose media capable of
growing a wide variety of microorganisms
Macconkey Agar - Pale red in color and is both selective and
differential media. The presence of crystal violet and bile salts
in its formulation restricts Gram- positive bacterial growth. As
such, only Gram- negative microbes can be grown.
Mannitol Salt Agar - This Agar is selective for Gram-positive
bacteria and can differentiate members of the Staphylococci
family.
True or False: Leprosy can move through the blood to other
areas including the brain, spine, and kidneys.
False: TB can move through the blood to other areas including
the brain, spine, and kidneys.
QUESTIONS WITH DETAILED ANSWERS GUARANTEED PASS
Describe the ONE main differences between lytic and
temperate phages.
Lytic bacteriophages replicate within the host bacteria until it
ruptures, whereas temperate (or lysogenic) phages primarily
exist in a non-replicative state that does not kill the host cell.
Describe TWO main characteristics of LYTIC bacteriophage
replication
1. It replicates in the host cell until the host ruptures
2. It replicates all proteins that it needs to assemble new virus
particles
Bacteria that have a curved rod morphology are referred to as
______.
Vibrio
You want to observe the movement of microorganisms that
are present in a sample of cheek cells obtained on a patient.
Briefly explain the technique that you would use and how it is
performed.
,A wet mount stain is indicated. A small liquid culture (usually
just a drop) containing a microorganism of interest is
prepared, added to a slide, and then covered with a glass
coverslip. The coverslip is present to both protect the objective
and the specimen while also holding the microorganism in
place. Wet mounts are used to observe the motility and
behavior of an organism.
Briefly explain why a researcher would use the simple stain
technique. How is it preformed and what does it identify
It uses a solution of positively charged dye, which binds to and
stains the negatively changed membrane of the
microorganism.
This technique can show size, shape, and arrangement of cells
What coronavirus-associated infection gave rise to
quarantining and travel restrictions in 2003?
May also ask about 2020
2003: SARS
2020: Covid-19
Match each type of agar plate to the corresponding general
, Characteristics of each.
LB Agar
Trypticase Soy Agar
Macconkey Agar
Mannitol Salt Agar
LB Agar - Also known as lysogeny broth, it is a multi-purpose
media capable of growing a wide variety of microorganisms
Trypticase Soy Agar - A multi-purpose media capable of
growing a wide variety of microorganisms
Macconkey Agar - Pale red in color and is both selective and
differential media. The presence of crystal violet and bile salts
in its formulation restricts Gram- positive bacterial growth. As
such, only Gram- negative microbes can be grown.
Mannitol Salt Agar - This Agar is selective for Gram-positive
bacteria and can differentiate members of the Staphylococci
family.
True or False: Leprosy can move through the blood to other
areas including the brain, spine, and kidneys.
False: TB can move through the blood to other areas including
the brain, spine, and kidneys.
