BIOD 152 AP2 LAB 4 EXAM SCRIPT 2026
TESTED QUESTIONS AND CORRECT ANSWERS
ALREADY PASSED
◉ (Lab 1) Micropipette. Answer: Size: < 1 mL
Function: measures micro-liter quantities
Accuracy: very accurate
Notes: common sizes include P20, P200, and P1000 (20, 200, 1000
microliters, respectively); plunger has two stops, first for
withdrawing fluid and the second for delivering fluid
◉ (Lab 1) Spectrophotometer. Answer: Function: used to measure
the amount of light that is absorbed (0-2) by or transmitted (0-100)
through a sample, at particular wavelengths of light; can use to
obtain an absorption spectrum and standard curve
◉ (Lab 1) Beer-Lambert law. Answer: The absorbance of a solution
is directly proportional to the concentration of molecules in the
solution, and the mathematical relationship between them
◉ (Lab 1) Aborption spectrum. Answer: The pattern of light
absorption over a range of wavelengths for a particular substance
, ◉ (Lab 1) Standard curve. Answer: The plot of the known
concentrations of a series of samples against their absorbances,
which can be used to find an unknown concentration
◉ (Lab 1) Scientific methods. Answer: Observation --> hypothesis --
> prediction --> experiment --> conclusion
◉ (Lab 1) Hypothesis. Answer: An educated guess; must be
falsifiable; typically an "If... then..." statement
◉ (Lab 1) Control. Answer: Identical to the experimental groups
EXCEPT lacking the variable of interest whose effect is being tested
◉ (Lab 1) Independent variable. Answer: The variable being
changed
◉ (Lab 1) Dependent variable. Answer: The variable being tested or
measured; depends on the independent variable
◉ (Lab 1) Percent error. Answer:
◉ (Lab 2) Diagram of a microscope. Answer:
◉ (Lab 2) Ocular lens. Answer: Function: 10x magnification
TESTED QUESTIONS AND CORRECT ANSWERS
ALREADY PASSED
◉ (Lab 1) Micropipette. Answer: Size: < 1 mL
Function: measures micro-liter quantities
Accuracy: very accurate
Notes: common sizes include P20, P200, and P1000 (20, 200, 1000
microliters, respectively); plunger has two stops, first for
withdrawing fluid and the second for delivering fluid
◉ (Lab 1) Spectrophotometer. Answer: Function: used to measure
the amount of light that is absorbed (0-2) by or transmitted (0-100)
through a sample, at particular wavelengths of light; can use to
obtain an absorption spectrum and standard curve
◉ (Lab 1) Beer-Lambert law. Answer: The absorbance of a solution
is directly proportional to the concentration of molecules in the
solution, and the mathematical relationship between them
◉ (Lab 1) Aborption spectrum. Answer: The pattern of light
absorption over a range of wavelengths for a particular substance
, ◉ (Lab 1) Standard curve. Answer: The plot of the known
concentrations of a series of samples against their absorbances,
which can be used to find an unknown concentration
◉ (Lab 1) Scientific methods. Answer: Observation --> hypothesis --
> prediction --> experiment --> conclusion
◉ (Lab 1) Hypothesis. Answer: An educated guess; must be
falsifiable; typically an "If... then..." statement
◉ (Lab 1) Control. Answer: Identical to the experimental groups
EXCEPT lacking the variable of interest whose effect is being tested
◉ (Lab 1) Independent variable. Answer: The variable being
changed
◉ (Lab 1) Dependent variable. Answer: The variable being tested or
measured; depends on the independent variable
◉ (Lab 1) Percent error. Answer:
◉ (Lab 2) Diagram of a microscope. Answer:
◉ (Lab 2) Ocular lens. Answer: Function: 10x magnification
