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MICR 2420: Final Exam Questions with Complete Solutions Updated.

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Wet mount - Answer A slide preparation technique where a specimen is placed on the slide with a drop of liquid Darkfield microscopy - Answer Type of microscopy where a bright image is seen on a dark background Brightfield microscopy - Answer Type of microscopy where a dark image is seen on a bright background Phase contrast microscopy - Answer Type of microscopy where higher contrast and higher-definition images are produced Gram stain - Answer A differential staining technique that distinguishes bacteria based upon their cell wall structure Acid fast stain - Answer A differential staining technique that differentiates cells that have mycolic acids in their gram-positive cell walls Endospore stain - Answer A differential staining technique that uses two stains to make bacterial endospores appear distinct from the rest of the cell Capsule stain - Answer A negative staining technique that stains around a bacterial capsule while leaving the capsule clear Scanning electron microscope - Answer A type of EM that forms an image of the cell surface and topography Transmission electron microscope - Answer A type of EM that focuses on a thinly spliced specimen Fluorescence microscope - Answer A microscope that uses natural fluorochromes or fluorescent stains to increase contrast

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MICR 2420: Final Exam Questions with
Complete Solutions 2025-2026
Updated.
Wet mount - Answer A slide preparation technique where a specimen is placed on the slide
with a drop of liquid



Darkfield microscopy - Answer Type of microscopy where a bright image is seen on a dark
background



Brightfield microscopy - Answer Type of microscopy where a dark image is seen on a bright
background



Phase contrast microscopy - Answer Type of microscopy where higher contrast and higher-
definition images are produced



Gram stain - Answer A differential staining technique that distinguishes bacteria based upon
their cell wall structure



Acid fast stain - Answer A differential staining technique that differentiates cells that have
mycolic acids in their gram-positive cell walls



Endospore stain - Answer A differential staining technique that uses two stains to make
bacterial endospores appear distinct from the rest of the cell



Capsule stain - Answer A negative staining technique that stains around a bacterial capsule
while leaving the capsule clear



Scanning electron microscope - Answer A type of EM that forms an image of the cell surface
and topography



Transmission electron microscope - Answer A type of EM that focuses on a thinly spliced
specimen



Fluorescence microscope - Answer A microscope that uses natural fluorochromes or
fluorescent stains to increase contrast

, Algae - Answer Unicellular or multicellular eukaryotic photosynthetic organisms, lack
vascular tissues and organs



Prokaryote - Answer An organism whose cell structure does not include a membrane-bound
nucleus



Eukaryote - Answer An organism made up of one or more cells that contain a membrane-
bound nucleus and organelles



Endosymbiotic theory - Answer The theory that mitochondria and chloroplasts arose as a
result of prokaryotic cells establishing a symbiotic relationship within a eukaryotic host



Cyanobacteria - Answer Phototrophic, chlorphyll-containing bacteria that produce large
amounts of gaseous oxygen



Plasmid - Answer Small, circular DNA molecule that is independent from the bacterial
chromosome



Plasma membrane - Answer Lipid bilayer with embedded proteins that defines the boundary
of a cell



Proton motive force - Answer Electrochemical gradient formed by the accumulation of
protons on one side of the membrane



Bacteriorhodopsin - Answer A protein present in the bacterium genus Halobacterium, it
transports protons across the cytoplasmic membrane when illuminated



Photosynthesis - Answer A process where phototrophic organisms convert solar energy into
chemical energy



Phototroph - Answer An organism that obtains its energy from light



Lithotroph - Answer An organism that uses inorganic chemicals as its electron source



Autotroph - Answer An organism that converts its inorganic carbon dioxide into organic
carbon



Heterotroph - Answer An organism that uses fixed organic carbon compounds as its carbon
source

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