1|Page
Straighterline Microbiology Lab BIO250L LABS
(2026/2027) VERIFIED EXAM || MOST RECENT EXAM
ACTUAL COMPLETE REAL EXAM QUESTIONS AND
CORRECT ANSWERS (VERIFIED ANSWERS)
ALREADY GRADED A+ | GUARANTEED SUCCESS!! |
BRAND NEW EXAM!!!
what three ways do you handle media? - Answer-make
sure that the top is on the bottom; plate is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
define agar - Answer-chemical derived from seaweed that
solidifies into a jelly-like substance and becomes a solid
what are two types of agar? - Answer-plates and slants
example of agar? - Answer-TSA--- trypticase soy agar
what is the air exposure procedure? - Answer-open the
plate to expose it to the air for several minutes then seal
with parafilm and place in an incubate at 22C for 24 hours
then 4C until the next week
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what is the objective of a surface swab? - Answer-to
demonstrate that their are microorganism and can only kill
some
what is sterilization and some examples? - Answer-when
you kill all the bacteria;
autoclave and radiation
what is autoclave? - Answer-example of sterilization when
you use heat and pressure
what is disinfection and an example? - Answer-only kills
some or most of the microorganisms; Lysol
when streaking plates, be sure to work close to the
Bunsen burner why?? - Answer-because hot air rises
creating a convection current therefore all the bacteria
floats up causing your work no to be contaminated
cross hatch pattern is what and what is it used in? -
Answer-surface swab and zigzag long was and up and
down
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what is the objective of air exposure? - Answer-to
demonstrate that microorganism are around us
define the medium? - Answer-liquid or gel designed to
expose bacteria
what are the two types if medium and what do they
provide? - Answer-broth and agar; they provide variable
growth conditions in the form of growth and stability
what is the objective of aseptic technique and transferring
culture - Answer-to create a subculture of Staphylococcus
Aureus
aseptic technique define and give some examples -
Answer-any procedure you do to help with contamination
wipe table, flame the loop, work close to Bunsen burner
whats a subculture? - Answer-transfer micro growth from
one medium to another
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whats a pure culture? - Answer-culture of bacteria
contaminating only one strain
what medium do we work with in aseptic technique? -
Answer-TSB- trypticase soy broth
TSB is used over TSA why? - Answer-TSB is liquid and
used to grow aerobic bacteria
S. aureus is found where? - Answer-skin and respiratory
tract
S. aureus is a common cause of - Answer-food poison,
skin infection, respiratory disease, and staff
what is the objective of a 4 way streak - Answer-to obtain
a pure culture and dilute the bacteria
what is the bacteria used in 4 way streak? - Answer-
micrococcus luteus
Straighterline Microbiology Lab BIO250L LABS
(2026/2027) VERIFIED EXAM || MOST RECENT EXAM
ACTUAL COMPLETE REAL EXAM QUESTIONS AND
CORRECT ANSWERS (VERIFIED ANSWERS)
ALREADY GRADED A+ | GUARANTEED SUCCESS!! |
BRAND NEW EXAM!!!
what three ways do you handle media? - Answer-make
sure that the top is on the bottom; plate is upside down
make sure to seal the plate with parafilm
store in correct temp for the bacteria
define agar - Answer-chemical derived from seaweed that
solidifies into a jelly-like substance and becomes a solid
what are two types of agar? - Answer-plates and slants
example of agar? - Answer-TSA--- trypticase soy agar
what is the air exposure procedure? - Answer-open the
plate to expose it to the air for several minutes then seal
with parafilm and place in an incubate at 22C for 24 hours
then 4C until the next week
,2|Page
what is the objective of a surface swab? - Answer-to
demonstrate that their are microorganism and can only kill
some
what is sterilization and some examples? - Answer-when
you kill all the bacteria;
autoclave and radiation
what is autoclave? - Answer-example of sterilization when
you use heat and pressure
what is disinfection and an example? - Answer-only kills
some or most of the microorganisms; Lysol
when streaking plates, be sure to work close to the
Bunsen burner why?? - Answer-because hot air rises
creating a convection current therefore all the bacteria
floats up causing your work no to be contaminated
cross hatch pattern is what and what is it used in? -
Answer-surface swab and zigzag long was and up and
down
,3|Page
what is the objective of air exposure? - Answer-to
demonstrate that microorganism are around us
define the medium? - Answer-liquid or gel designed to
expose bacteria
what are the two types if medium and what do they
provide? - Answer-broth and agar; they provide variable
growth conditions in the form of growth and stability
what is the objective of aseptic technique and transferring
culture - Answer-to create a subculture of Staphylococcus
Aureus
aseptic technique define and give some examples -
Answer-any procedure you do to help with contamination
wipe table, flame the loop, work close to Bunsen burner
whats a subculture? - Answer-transfer micro growth from
one medium to another
, 4|Page
whats a pure culture? - Answer-culture of bacteria
contaminating only one strain
what medium do we work with in aseptic technique? -
Answer-TSB- trypticase soy broth
TSB is used over TSA why? - Answer-TSB is liquid and
used to grow aerobic bacteria
S. aureus is found where? - Answer-skin and respiratory
tract
S. aureus is a common cause of - Answer-food poison,
skin infection, respiratory disease, and staff
what is the objective of a 4 way streak - Answer-to obtain
a pure culture and dilute the bacteria
what is the bacteria used in 4 way streak? - Answer-
micrococcus luteus
