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Mod 5 DNA Replication + midterm exam with comprehensive questions and well elaborated correct answers graded A+

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Mod 5 DNA Replication + midterm exam with comprehensive questions and well elaborated correct answers graded A+

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RePL CASA
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RePL CASA

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Mod 5 DNA Replication + midterm exam with
comprehensive questions and well elaborated
correct answers graded A+
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, In semi-conservative replication, - correct ans:each daughter chromosome is a hybrid with one parental
strand and one new strand.



DNA replication occurs at a much faster rate in eukaryotes than in prokaryotes. This statement is: -
correct ans:False, occurs much faster in prokaryotes (E.coli replicates in 40min), and euk usually take like
hours



Which statement is true regarding replication of the E.coli chromosome? - correct ans:one origin of
replication and two replication forks



When viewed by electron microscopy, open complex DNA is called: - correct ans:Replication bubble



Which of the following E.coli enzymes functions as a helicase? - correct ans:DnaB



In eukaryotes, each chromosome has a unique origin of replication, and replication occurs through bi-
directional synthesis. This statement is: - correct ans:False, euks have multiple origins



In eukaryotes, binding of pre-replication complex proteins to ARS occurs during: - correct ans:d. G1
phase



DNA polymerase adds nucleotides to the: - correct ans:3'OH



In E.coli, which DNA polymerase is also called replicase? - correct ans:Pol III involved in repair(3 to 5) and
synthesis for replication (5 to 3)



In E.coli, which DNA polymerase also has nick translation activity? - correct ans:Pol I

remember this one by Pol "I" indicates N"i"ck = makes one nick but ultimately removes a stretch of
nucleotides in the same direction as DNA synthesis. Then, new dNTPs are introduced into the region.
Ligase comes and seals the remaining gap.



Okazaki fragments only occur in prokaryotes. This statement is: - correct ans:False

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