BIOS 312 - EXAM #2 QUESTIONS WITH
CORRECT ANSWERS
In size exclusion chromatography, the ____________ phase is a solid and the
| | | | | | | | | | | |
__________ phase is a liquid. | | | |
A. mobile; stationary
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B. stationary; mobile
| |
C. exclusion; exchange
| |
D. exchange; exclusion - CORRECT ANSWER✔✔-B. stationary; mobile
| | | | | | |
Describe how a size exclusion chromatography column works. Your answer must
| | | | | | | | | | |
include appropriate use of the terms beads, molecules, buffer, and fractions for
| | | | | | | | | | | |
you to receive full credit. - CORRECT ANSWER✔✔-In size exclusion
| | | | | | | | | |
chromatography a column filled with porous beads will be used to create
| | | | | | | | | | | |
fractions that contain a certain protein molecule. A buffer will be poured into the
| | | | | | | | | | | | | |
column to elute the sample through the column. Smaller molecules will get
| | | | | | | | | | | |
trapped in the beads while larger ones will run down and be collected.
| | | | | | | | | | | |
During size exclusion chromatography of two proteins, the 30 kDa / 100 kDa
| | | | | | | | | | | | |
protein will be found in the last fractions collected. - CORRECT ANSWER✔✔-
| | | | | | | | | | |
30kDa
Describe how affinity chromatography works. Your answer must include
| | | | | | | | |
appropriate use of the term antibody or enzyme and column for full credit. -
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, CORRECT ANSWER✔✔-Antibodies will be present in the beads and desired
| | | | | | | | | |
protein will bind to them trapping it. This will be added to a column, everything
| | | | | | | | | | | | | | |
but the protein desired will pass. Protein desired will bind to antibody.
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During size exclusion chromatography, a molecule that is smaller than the bead
| | | | | | | | | | | |
pore size will travel through ____________ .
| | | | | |
A. the void volume
| | |
B. the pore volume
| | |
C. both the void volume and the pore volume
| | | | | | | |
D. neither the void volume nor the pore volume - CORRECT ANSWER✔✔-C. both
| | | | | | | | | | | | |
the void volume and the pore volume
| | | | | |
You have a mixture of 2 proteins. Protein A has a molecular weight of 27 kDa and
| | | | | | | | | | | | | | | | |
Protein B is 110 kDa. Knowing only this information, which type of
| | | | | | | | | | | |
chromatography would be best for separating the two proteins? | | | | | | | |
A. Ion exchange chromatography
| | |
B. Hydrophobic interaction chromatography
| | |
C. Size exclusion chromatography
| | |
D. Affinity chromatography - CORRECT ANSWER✔✔-C. Size exclusion
| | | | | | | |
chromatography
You have a mixture of 2 proteins. Protein A is positively charged and Protein B is
| | | | | | | | | | | | | | | |
negatively charged. Knowing only this information, which type of chromatography
| | | | | | | | |
would be best for separating the two proteins?
| | | | | | | |
A. Ion exchange chromatography
| | |
B. Hydrophobic interaction chromatography
| | |
CORRECT ANSWERS
In size exclusion chromatography, the ____________ phase is a solid and the
| | | | | | | | | | | |
__________ phase is a liquid. | | | |
A. mobile; stationary
| |
B. stationary; mobile
| |
C. exclusion; exchange
| |
D. exchange; exclusion - CORRECT ANSWER✔✔-B. stationary; mobile
| | | | | | |
Describe how a size exclusion chromatography column works. Your answer must
| | | | | | | | | | |
include appropriate use of the terms beads, molecules, buffer, and fractions for
| | | | | | | | | | | |
you to receive full credit. - CORRECT ANSWER✔✔-In size exclusion
| | | | | | | | | |
chromatography a column filled with porous beads will be used to create
| | | | | | | | | | | |
fractions that contain a certain protein molecule. A buffer will be poured into the
| | | | | | | | | | | | | |
column to elute the sample through the column. Smaller molecules will get
| | | | | | | | | | | |
trapped in the beads while larger ones will run down and be collected.
| | | | | | | | | | | |
During size exclusion chromatography of two proteins, the 30 kDa / 100 kDa
| | | | | | | | | | | | |
protein will be found in the last fractions collected. - CORRECT ANSWER✔✔-
| | | | | | | | | | |
30kDa
Describe how affinity chromatography works. Your answer must include
| | | | | | | | |
appropriate use of the term antibody or enzyme and column for full credit. -
| | | | | | | | | | | | | |
, CORRECT ANSWER✔✔-Antibodies will be present in the beads and desired
| | | | | | | | | |
protein will bind to them trapping it. This will be added to a column, everything
| | | | | | | | | | | | | | |
but the protein desired will pass. Protein desired will bind to antibody.
| | | | | | | | | | |
During size exclusion chromatography, a molecule that is smaller than the bead
| | | | | | | | | | | |
pore size will travel through ____________ .
| | | | | |
A. the void volume
| | |
B. the pore volume
| | |
C. both the void volume and the pore volume
| | | | | | | |
D. neither the void volume nor the pore volume - CORRECT ANSWER✔✔-C. both
| | | | | | | | | | | | |
the void volume and the pore volume
| | | | | |
You have a mixture of 2 proteins. Protein A has a molecular weight of 27 kDa and
| | | | | | | | | | | | | | | | |
Protein B is 110 kDa. Knowing only this information, which type of
| | | | | | | | | | | |
chromatography would be best for separating the two proteins? | | | | | | | |
A. Ion exchange chromatography
| | |
B. Hydrophobic interaction chromatography
| | |
C. Size exclusion chromatography
| | |
D. Affinity chromatography - CORRECT ANSWER✔✔-C. Size exclusion
| | | | | | | |
chromatography
You have a mixture of 2 proteins. Protein A is positively charged and Protein B is
| | | | | | | | | | | | | | | |
negatively charged. Knowing only this information, which type of chromatography
| | | | | | | | |
would be best for separating the two proteins?
| | | | | | | |
A. Ion exchange chromatography
| | |
B. Hydrophobic interaction chromatography
| | |
