BIOS 312 - EXAM #1 QUESTIONS WITH
COMPLETE SOLUTIONS
smallest subunit of a polypeptide - CORRECT ANSWER✔✔-amino acid
| | | | | | | |
After a polypeptide has folded, _______ amino acids will be found in the interior -
| | | | | | | | | | | | | |
CORRECT ANSWER✔✔-hydrophobic
| |
After a polypeptide has folded, _______ amino acids will be found on the exterior
| | | | | | | | | | | | |
- CORRECT ANSWER✔✔-hydrophilic
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SDS is a - CORRECT ANSWER✔✔-detergent
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Define the term "cell lysis." - CORRECT ANSWER✔✔-break cells to avoid
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denaturing or degrading of proteins/DNA | | | |
List two methods for releasing proteins from cells and explain - CORRECT
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ANSWER✔✔-break cell apart by vibrations | | | | |
using hypotonic buffer so the cell swells and bursts
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What is the function of the NP-40 detergent in the extraction buffer - CORRECT
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ANSWER✔✔-Helps disrupt the cell membrane in the tissue | | | | | | |
, Explain in your own words what "high-quality" and "high-yield" mean when
| | | | | | | | | | |
referring to protein extraction - CORRECT ANSWER✔✔-High yield = solubilized
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most protein |
High quality = inhibiting proteases to stop denaturing of proteins
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Describe two things you will do to ensure you have a "high-yield" extract -
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CORRECT ANSWER✔✔-Mince and Vortex tissue
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Describe two things you will do to ensure you have a "high-quality" extract. -
| | | | | | | | | | | | | |
CORRECT ANSWER✔✔-Keeping on ice = inhibit proteases
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Centrifuge = separate soluble proteins from tissue debris
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"SDS-PAGE" (what does it stand for?) - CORRECT ANSWER✔✔-Sodium Dodecyl
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Sulfate Polyacrylamide Gel Electrophoresis
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An SDS-coated protein has a negative / positive charge. - CORRECT ANSWER✔✔-
| | | | | | | | | | |
negative
The stacking gel in SDS-PAGE typically contains __% acrylamide. - CORRECT
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ANSWER✔✔-4%
The resolving gel in SDS-PAGE typically contains ___% acrylamide. - CORRECT
| | | | | | | | | | |
ANSWER✔✔-8-15%
COMPLETE SOLUTIONS
smallest subunit of a polypeptide - CORRECT ANSWER✔✔-amino acid
| | | | | | | |
After a polypeptide has folded, _______ amino acids will be found in the interior -
| | | | | | | | | | | | | |
CORRECT ANSWER✔✔-hydrophobic
| |
After a polypeptide has folded, _______ amino acids will be found on the exterior
| | | | | | | | | | | | |
- CORRECT ANSWER✔✔-hydrophilic
| | |
SDS is a - CORRECT ANSWER✔✔-detergent
| | | | |
Define the term "cell lysis." - CORRECT ANSWER✔✔-break cells to avoid
| | | | | | | | | | |
denaturing or degrading of proteins/DNA | | | |
List two methods for releasing proteins from cells and explain - CORRECT
| | | | | | | | | | | |
ANSWER✔✔-break cell apart by vibrations | | | | |
using hypotonic buffer so the cell swells and bursts
| | | | | | | |
What is the function of the NP-40 detergent in the extraction buffer - CORRECT
| | | | | | | | | | | | | |
ANSWER✔✔-Helps disrupt the cell membrane in the tissue | | | | | | |
, Explain in your own words what "high-quality" and "high-yield" mean when
| | | | | | | | | | |
referring to protein extraction - CORRECT ANSWER✔✔-High yield = solubilized
| | | | | | | | | |
most protein |
High quality = inhibiting proteases to stop denaturing of proteins
| | | | | | | | |
Describe two things you will do to ensure you have a "high-yield" extract -
| | | | | | | | | | | | | |
CORRECT ANSWER✔✔-Mince and Vortex tissue
| | | |
Describe two things you will do to ensure you have a "high-quality" extract. -
| | | | | | | | | | | | | |
CORRECT ANSWER✔✔-Keeping on ice = inhibit proteases
| | | | | |
Centrifuge = separate soluble proteins from tissue debris
| | | | | | |
"SDS-PAGE" (what does it stand for?) - CORRECT ANSWER✔✔-Sodium Dodecyl
| | | | | | | | | |
Sulfate Polyacrylamide Gel Electrophoresis
| | |
An SDS-coated protein has a negative / positive charge. - CORRECT ANSWER✔✔-
| | | | | | | | | | |
negative
The stacking gel in SDS-PAGE typically contains __% acrylamide. - CORRECT
| | | | | | | | | | |
ANSWER✔✔-4%
The resolving gel in SDS-PAGE typically contains ___% acrylamide. - CORRECT
| | | | | | | | | | |
ANSWER✔✔-8-15%
