(Microbio Basset) Lab Exam 1 – Questions With
Proper Solutions
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Terms in this set (111)
Long hair must be tied back because C. It is a potential source of contamination
_____________________. (Select all that apply) D. It is a likely target target for fire if Bunsen Burners
A. It would never contaminate cultures are used
B. It will ruin your microscope
C. It is a potential source of
contamination
D. It is a likely target target for fire if
Bunsen Burners are used
The function of agar in the culture B. Act as a solidifying agent
media is
EVEN RELATIVELY "HARMLESS" A. True
BACTERIA WHEN CULTIVATED ON A
GROWTH MEDIUM, ARE IN
SUFFICIENT NUMBERS TO
CONSTITUTE A HEALTH HAZARD.
A. True
B. False
Plates must be ________ and then A. Labeled
incubated. Select all that apply. B. Inverted
A. labeled
B. inverted
C. soaked in bleach
D. shaken
Total magnification = magnification by C. ocular...objective
the _____ lens x magnification by the
___lens.
,_________ is used for cleaning the D. Lens Paper
condenser and objective lenses.
When you start, the condenser should A. True
be nearly even with the stage and the
iris diaphragm should be open.
A. True
B. False
Parfocal means that the B. Image should remain mostly in focus as you
_______________________. increase in magnification
Before advancing to a higher A. in focus
objective, the specimen must be B. centered
_____________. (Select all that apply)
A. in focus
B. centered
C. out of the center
D. to the right on the slid
If you are working with a bacterial D. Oil immersion
smear, you must use the ________lens in
the final step.
It is extremely important to work A. True
through the 10X, then 40X objectives,
adjusting the fine focus and lighting
for each before advancing to oil
immersion.
A. True
B. False
The scan, 10X and 40X lenses and A. True
other parts of the microscope should
not get any oil on them.
A. True...This is very important
B. False...All lenses should be used
with oil
, The stage should be moved down to B. False
add oil to the slide.
A. True
B. False
To remove the oil from the lenses, D. Lens Paper
______ should be used.
If you see rods under the microscope, A. Bacillus
the morphology is
If you see spheres under the C. Coccus
microscope, the morphology of the
bacteria is
Bacteria have a ____________________________ C. Negatively-Charged
surface.
If you look in the microscope and see Strepto
chains of organisms, the prefix is
termed _______.
If you look in the microscope and see Staphylo
clusters of organisms, the prefix is
termed ________.
The name of the dye used in the Nigrosin....acidic
negative stain is ________ and it is
________________.
The negative staining technique is Determine morphology and cellular arrangement
used to that are too delicate to withstand heat-fixing
Determine the accurate size since there is minimal
cell shrinkage
Where do you discard your used agar In the biohazard bags
plates, swabs, and other contaminated
non-sharp items?
Proper Solutions
Save Groups
Terms in this set (111)
Long hair must be tied back because C. It is a potential source of contamination
_____________________. (Select all that apply) D. It is a likely target target for fire if Bunsen Burners
A. It would never contaminate cultures are used
B. It will ruin your microscope
C. It is a potential source of
contamination
D. It is a likely target target for fire if
Bunsen Burners are used
The function of agar in the culture B. Act as a solidifying agent
media is
EVEN RELATIVELY "HARMLESS" A. True
BACTERIA WHEN CULTIVATED ON A
GROWTH MEDIUM, ARE IN
SUFFICIENT NUMBERS TO
CONSTITUTE A HEALTH HAZARD.
A. True
B. False
Plates must be ________ and then A. Labeled
incubated. Select all that apply. B. Inverted
A. labeled
B. inverted
C. soaked in bleach
D. shaken
Total magnification = magnification by C. ocular...objective
the _____ lens x magnification by the
___lens.
,_________ is used for cleaning the D. Lens Paper
condenser and objective lenses.
When you start, the condenser should A. True
be nearly even with the stage and the
iris diaphragm should be open.
A. True
B. False
Parfocal means that the B. Image should remain mostly in focus as you
_______________________. increase in magnification
Before advancing to a higher A. in focus
objective, the specimen must be B. centered
_____________. (Select all that apply)
A. in focus
B. centered
C. out of the center
D. to the right on the slid
If you are working with a bacterial D. Oil immersion
smear, you must use the ________lens in
the final step.
It is extremely important to work A. True
through the 10X, then 40X objectives,
adjusting the fine focus and lighting
for each before advancing to oil
immersion.
A. True
B. False
The scan, 10X and 40X lenses and A. True
other parts of the microscope should
not get any oil on them.
A. True...This is very important
B. False...All lenses should be used
with oil
, The stage should be moved down to B. False
add oil to the slide.
A. True
B. False
To remove the oil from the lenses, D. Lens Paper
______ should be used.
If you see rods under the microscope, A. Bacillus
the morphology is
If you see spheres under the C. Coccus
microscope, the morphology of the
bacteria is
Bacteria have a ____________________________ C. Negatively-Charged
surface.
If you look in the microscope and see Strepto
chains of organisms, the prefix is
termed _______.
If you look in the microscope and see Staphylo
clusters of organisms, the prefix is
termed ________.
The name of the dye used in the Nigrosin....acidic
negative stain is ________ and it is
________________.
The negative staining technique is Determine morphology and cellular arrangement
used to that are too delicate to withstand heat-fixing
Determine the accurate size since there is minimal
cell shrinkage
Where do you discard your used agar In the biohazard bags
plates, swabs, and other contaminated
non-sharp items?
