BIOS 251 Lab Practical Exam 4 (Latest
Update ) Anatomy and
Physiology IV with Lab | Questions &
Answers | 100% Correct | Grade A -
Chamberlain
1. Which microscope lens is used for initial scanning of specimens?
A. 4x
B. 10x
C. 40x
D. 100x
Correct answer: A
Rationale: The 4x objective provides the widest field of view to locate
specimens.
2. Which pipette is used for accurate microliter transfers?
A. Micropipette
B. Volumetric pipette
C. Pasteur pipette
D. Graduated pipette
Correct answer: A
Rationale: Micropipettes are designed for precise measurement of very small
volumes.
,3. Which solution indicates the presence of protein?
A. Biuret reagent
B. Benedict’s solution
C. Iodine
D. Sudan III
Correct answer: A
Rationale: Proteins react with Biuret reagent to produce a purple color.
4. Which stain is used to detect lipids in cells?
A. Sudan III
B. Hematoxylin
C. Eosin
D. Iodine
Correct answer: A
Rationale: Sudan III binds lipids and stains them red-orange.
5. Which technique separates plasma from red blood cells?
A. Centrifugation
B. Filtration
C. Chromatography
D. Microscopy
Correct answer: A
Rationale: Centrifugation separates blood components based on density.
6. Which stain differentiates nuclei and cytoplasm?
,A. Hematoxylin & Eosin
B. Sudan III
C. Benedict’s solution
D. Iodine
Correct answer: A
Rationale: Hematoxylin stains nuclei blue; Eosin stains cytoplasm pink.
7. Which solution tests for reducing sugars?
A. Benedict’s solution
B. Biuret reagent
C. Iodine
D. Sudan III
Correct answer: A
Rationale: Reducing sugars reduce copper ions forming a colored precipitate.
8. Which part of the microscope allows switching between objectives?
A. Nosepiece
B. Stage
C. Arm
D. Base
Correct answer: A
Rationale: The revolving nosepiece holds multiple objective lenses.
9. Which microscope component adjusts light intensity?
A. Diaphragm
B. Stage
, C. Nosepiece
D. Arm
Correct answer: A
Rationale: The diaphragm regulates the amount of light passing through the
specimen.
10. Which pipette is suitable for transferring a fixed 10 mL volume?
A. Volumetric pipette
B. Micropipette
C. Pasteur pipette
D. Graduated pipette
Correct answer: A
Rationale: Volumetric pipettes are calibrated for precise fixed volumes.
11. Which solution tests for the presence of starch?
A. Iodine
B. Biuret reagent
C. Benedict’s solution
D. Sudan III
Correct answer: A
Rationale: Iodine reacts with starch to form a blue-black complex.
12. Which microscope lens provides high magnification for bacterial
observation?
A. 100x (oil immersion)
B. 4x
Update ) Anatomy and
Physiology IV with Lab | Questions &
Answers | 100% Correct | Grade A -
Chamberlain
1. Which microscope lens is used for initial scanning of specimens?
A. 4x
B. 10x
C. 40x
D. 100x
Correct answer: A
Rationale: The 4x objective provides the widest field of view to locate
specimens.
2. Which pipette is used for accurate microliter transfers?
A. Micropipette
B. Volumetric pipette
C. Pasteur pipette
D. Graduated pipette
Correct answer: A
Rationale: Micropipettes are designed for precise measurement of very small
volumes.
,3. Which solution indicates the presence of protein?
A. Biuret reagent
B. Benedict’s solution
C. Iodine
D. Sudan III
Correct answer: A
Rationale: Proteins react with Biuret reagent to produce a purple color.
4. Which stain is used to detect lipids in cells?
A. Sudan III
B. Hematoxylin
C. Eosin
D. Iodine
Correct answer: A
Rationale: Sudan III binds lipids and stains them red-orange.
5. Which technique separates plasma from red blood cells?
A. Centrifugation
B. Filtration
C. Chromatography
D. Microscopy
Correct answer: A
Rationale: Centrifugation separates blood components based on density.
6. Which stain differentiates nuclei and cytoplasm?
,A. Hematoxylin & Eosin
B. Sudan III
C. Benedict’s solution
D. Iodine
Correct answer: A
Rationale: Hematoxylin stains nuclei blue; Eosin stains cytoplasm pink.
7. Which solution tests for reducing sugars?
A. Benedict’s solution
B. Biuret reagent
C. Iodine
D. Sudan III
Correct answer: A
Rationale: Reducing sugars reduce copper ions forming a colored precipitate.
8. Which part of the microscope allows switching between objectives?
A. Nosepiece
B. Stage
C. Arm
D. Base
Correct answer: A
Rationale: The revolving nosepiece holds multiple objective lenses.
9. Which microscope component adjusts light intensity?
A. Diaphragm
B. Stage
, C. Nosepiece
D. Arm
Correct answer: A
Rationale: The diaphragm regulates the amount of light passing through the
specimen.
10. Which pipette is suitable for transferring a fixed 10 mL volume?
A. Volumetric pipette
B. Micropipette
C. Pasteur pipette
D. Graduated pipette
Correct answer: A
Rationale: Volumetric pipettes are calibrated for precise fixed volumes.
11. Which solution tests for the presence of starch?
A. Iodine
B. Biuret reagent
C. Benedict’s solution
D. Sudan III
Correct answer: A
Rationale: Iodine reacts with starch to form a blue-black complex.
12. Which microscope lens provides high magnification for bacterial
observation?
A. 100x (oil immersion)
B. 4x
