AACE CCT PRIMER EXAMS SET 2026
COMPLETE TEST BANK QUESTIONS AND
SOLUTIONS ACCURATE A+
●● Simply, what does a primer do? Answer: specifies the exact DNA
product to be amplified
●● How do primers work with or for DNA polymerase? Answer:
primers serve as an extension point for DNA polymerase to build upon
●● when will annealing occur between primers and target DNA?
Answer: when the complementary sequence matches up A & G, T & C
●● How does DNA polymerase extend the primers? Answer: by adding
nucleotides to the developing DNA strand
●● Why do you want the GC content level to be < 50%? Answer: Most
housekeeping genes and tumor-suppressor genes are GC wish, thus the
DNA is less amenable to amplification, also a lot of stop codons are GC
rich
●● what is a primer dimer? Answer: molecules that have hybridized to
each other due to strings of complementary bases in the primers
COMPLETE TEST BANK QUESTIONS AND
SOLUTIONS ACCURATE A+
●● Simply, what does a primer do? Answer: specifies the exact DNA
product to be amplified
●● How do primers work with or for DNA polymerase? Answer:
primers serve as an extension point for DNA polymerase to build upon
●● when will annealing occur between primers and target DNA?
Answer: when the complementary sequence matches up A & G, T & C
●● How does DNA polymerase extend the primers? Answer: by adding
nucleotides to the developing DNA strand
●● Why do you want the GC content level to be < 50%? Answer: Most
housekeeping genes and tumor-suppressor genes are GC wish, thus the
DNA is less amenable to amplification, also a lot of stop codons are GC
rich
●● what is a primer dimer? Answer: molecules that have hybridized to
each other due to strings of complementary bases in the primers
