ASCP HEMATOLOGY LABORATORY
TESTING FINAL PAPER PRACTICE
SOLUTION TESTED QUESTIONS WITH
COMPLETE ANSWERS
●● Erythropoiesis:
Liver, spleen
Answer: 3-6 mo of gestation
-Liver is primary site
●● Erythropoiesis:
7 mo of gestation—age 4 yr
Answer: Bone marrow. All marrow is active
●● Erythropoiesis:
Adult
Answer: bone marrow
-only active sites are Skull, Ribs, Sternum, Vertebrae, and Pelvis
- the shafts of the long bone are filled with fat (the fatty marrow may be
reactivated to compensate for anemia)
,●● Changes During Cell Maturation: Cytoplasm
Less basophilic due to loss of ---. Granulocytes produce granules.
Erythrocytes become pink due to ---- production.
Answer: Changes During Cell Maturation: Cytoplasm
-Less basophilic due to loss of RNA. Granulocytes produce granules.
Erythrocytes become pink due to Hgb production.
●● Changes During Cell Maturation: Nucleus
-Becomes smaller. Nuclear chromatin ----. Nucleoli disappear. In
granulocytic series, -- indents, then segments. In erythrocytic series, the
--- is extruded.
Answer: Changes During Cell Maturation: Nucleus
-Becomes smaller. Nuclear chromatin condenses. Nucleoli disappear. In
granulocytic series, nucleus indents, then segments. In erythrocytic
series, nucleus is extruded.
●● RUBRIBLAST TERMINOLOGY:
Rubriblast
,Answer: Pronormoblast
- N:C ratio 8:1. Royal blue cytoplasm.
-Fine chromatin.
-1-2 nucleoli. Central
-Normally confined to bone marrow.
●● RUBRIBLAST TERMINOLOGY:
Prorubricyte
Answer: Basophilic normoblast
-N:C ratio 6:1.
-Chromatin is coarser with slightly visible parachromatin.
-Nucleoli usually not visible.
-Normally confined to bone marrow.
●● RUBRIBLAST TERMINOLOGY:
Rubricyte
Answer: Polychromatophilic normoblast: The Last stage to divide.
, -N:C ratio 4:1.
-Cytoplasm is polychromatophilic due to hemoglobin production.
-Chromatin is clumped with distinct areas of parachromatin.
-Normally confined to bone marrow.
-Note that the cytoplasm color is now a blue/gray rather than the deep
midnight blue of the basophilic pronormoblast stage.
●● RUBRIBLAST TERMINOLOGY:
Metarubricyte
Answer: Orthochromic normoblast:-Last nucleated stage.
N:C ratio 1:2. Nucleus is pyknotic.
-Normally confined to bone marrow.
●● RUBRIBLAST TERMINOLOGY:
Reticulocyte
Answer: Polychromatophilic erythrocyte :No Nucleus
-Cytoplasm is diffusely basophilic (bluish
tinge).
TESTING FINAL PAPER PRACTICE
SOLUTION TESTED QUESTIONS WITH
COMPLETE ANSWERS
●● Erythropoiesis:
Liver, spleen
Answer: 3-6 mo of gestation
-Liver is primary site
●● Erythropoiesis:
7 mo of gestation—age 4 yr
Answer: Bone marrow. All marrow is active
●● Erythropoiesis:
Adult
Answer: bone marrow
-only active sites are Skull, Ribs, Sternum, Vertebrae, and Pelvis
- the shafts of the long bone are filled with fat (the fatty marrow may be
reactivated to compensate for anemia)
,●● Changes During Cell Maturation: Cytoplasm
Less basophilic due to loss of ---. Granulocytes produce granules.
Erythrocytes become pink due to ---- production.
Answer: Changes During Cell Maturation: Cytoplasm
-Less basophilic due to loss of RNA. Granulocytes produce granules.
Erythrocytes become pink due to Hgb production.
●● Changes During Cell Maturation: Nucleus
-Becomes smaller. Nuclear chromatin ----. Nucleoli disappear. In
granulocytic series, -- indents, then segments. In erythrocytic series, the
--- is extruded.
Answer: Changes During Cell Maturation: Nucleus
-Becomes smaller. Nuclear chromatin condenses. Nucleoli disappear. In
granulocytic series, nucleus indents, then segments. In erythrocytic
series, nucleus is extruded.
●● RUBRIBLAST TERMINOLOGY:
Rubriblast
,Answer: Pronormoblast
- N:C ratio 8:1. Royal blue cytoplasm.
-Fine chromatin.
-1-2 nucleoli. Central
-Normally confined to bone marrow.
●● RUBRIBLAST TERMINOLOGY:
Prorubricyte
Answer: Basophilic normoblast
-N:C ratio 6:1.
-Chromatin is coarser with slightly visible parachromatin.
-Nucleoli usually not visible.
-Normally confined to bone marrow.
●● RUBRIBLAST TERMINOLOGY:
Rubricyte
Answer: Polychromatophilic normoblast: The Last stage to divide.
, -N:C ratio 4:1.
-Cytoplasm is polychromatophilic due to hemoglobin production.
-Chromatin is clumped with distinct areas of parachromatin.
-Normally confined to bone marrow.
-Note that the cytoplasm color is now a blue/gray rather than the deep
midnight blue of the basophilic pronormoblast stage.
●● RUBRIBLAST TERMINOLOGY:
Metarubricyte
Answer: Orthochromic normoblast:-Last nucleated stage.
N:C ratio 1:2. Nucleus is pyknotic.
-Normally confined to bone marrow.
●● RUBRIBLAST TERMINOLOGY:
Reticulocyte
Answer: Polychromatophilic erythrocyte :No Nucleus
-Cytoplasm is diffusely basophilic (bluish
tinge).
