ASCP HEMATOLOGY POLANSKY CARDS EXAM SCRIPT COMPREHENSIVE STUDY GUIDE QUESTIONS WITH ACCURATE SOLUTIONS

ASCP HEMATOLOGY POLANSKY CARDS
EXAM SCRIPT COMPREHENSIVE STUDY
GUIDE QUESTIONS WITH ACCURATE
SOLUTIONS

●● Erythropoiesis: After 7 months where are RBCs made?
Answer: Bone marrow.


●● Erythropoiesis: In Adults, where are active sites for production?
Answer: Skull, Ribs, Sternum, Vertebrae, and Pelvis


●● RUBRIBLAST TERMINOLOGY:


Red Cells other names PBPOLORTHOPOL
Answer: Pronormoblast
Basophilic normo
Polychromatophilic normo
Orthochromic normo
Polychromatophilic normo


●● How to observe reticulocytes?

,Answer: New methlyne blue for presence of RNA


●● What Type of Hemoglobin?
2α , 2 β chains
Answer: Hemoglobin A


Adults: > 95%
Newborn: 20%


●● What Type of Hemoglobin?


2α , 2 δ chains
(two alpha and two delta chains)
Answer: Hemoglobin A2


Adults: 1.5%-3.7%
Newborn: <1%


●● What Type of Hemoglobin?


2α , 2 γ chains
Answer: Hemoglobin F

,Adults: <2%
Newborn: 50-85%


●● Hemoglobin SC: In which position of β chain?
Answer: 6th


●● Hemoglobin S: ----- substituted for glutamic acid? in --- position of β
chain
Answer: Valine


●● Hemoglobin C: ---- substituted for glutamic acid? ----position of β
chain
Answer: Lysine


●● Hemoglobin Electrophoresis: Cellulose Acetate pH
Answer: 8.6


●● Hemoglobin Electrophoresis: Citrate Acetate pH =
Answer: 6.2


●● Hemoglobin Electrophoresis:

, Migration on Cellulose Acetate:
From Cathode to Anode (military job code!)
Answer: Hemoglobin Electrophoresis:


-Origin at Cathode(-) "think steps to walking"


-Crawl: Hemoglobin C/A2 (on top)
-Slow: Hemoglobin S
-Fast: Hemoglobin F
-Accelerate: Hemoglobin A


●● Hemoglobin Electrophoresis:


Migration on Citrate Acetate:
From Cathode to Anode (finger always some ...)
Answer: Hemoglobin Electrophoresis:


-Origin in middle "think steps to walking" from anode and switch at
Cathode
Anode
-Crawl: Hemoglobin C
-Slow: Hemoglobin S