ACE ASCP ACTUAL EXAM PAPER 2026
QUESTIONS WITH ANSWERS GRADED A+
◍ Characteristic: Red Bull's Eye colony in CIN Medium: Klebsiella
pneumoniae Serratia marcescens Clostridium difficile Yersinia
enterocolitica.
Answer: Feedback The characteristic deep red center with a transparent
margin, or "bull's-eye" appearance of Yersinia and Aeromonas colonies is
important for identification, and is due to the presence of mannitol. Y.
enterocolitica ferments the mannitol in the medium, producing an acid pH
which gives the colonies their red color and the "bull's eye" appearance.
◍ D. Chronic Lymphocytic Leukemia (CLL)Feedback Smudge cells are
remnants of cells that lack any identifiable cytoplasmic membrane or
nuclear structure. Smudge cells, also called basket cells, are most often
associated with abnormally fragile lymphocytes in disorders such as chronic
lymphocytic leukemia (CLL)..
Answer: Smudge cells are seen in what type of leukemia? Acute
Myelogenous Leukemia (AML) Chronic Myelogenous Leukemia (CML)
Acute Lymphocytic Leukemia (ALL) Chronic Lymphocytic Leukemia
(CLL)
◍ B. Bordetella pertussisFeedback Stages of Whooping cough: 1. Catarrhal, 2.
Paroxysmal, 3. Convalescence, preferred sample of choice of isolation of
B. pertussis is nasopharyngeal swab..
Answer: "Whooping cough bacillus": Bordetella bronchiseptica Bordetella
pertussis Haemophilus aegypticus NOTA
◍ Indole: negativeMR: negativeLDC: negativeODC: positiveADH: positive
K. pneumoniae
K. oxytoca
, E. cloacae
E. aerogenes.
Answer: C. E cloacae
◍ Rheumatoid Factor:
A. IgG that binds to the Fc portion of abnormal IgM
B. IgM that binds to the Fc portion of abnormal IgG
C. A and B
D. None of the above.
Answer: B. IgM that binds to the Fc portion of abnormal IgG
◍ Minimum competency.
Answer: ACE certification provides the ability to work in the fitness field.
This requirement is referred to as what?
◍ B. Release of ADPFeedback Under normal conditions, small disk-shape
platelets circulate in the blood freely and without interaction with one
another. ADP is stored in dense bodies inside blood platelets and is released
upon platelet activation. ADP interacts with a family of ADP receptors
found on platelets (P2Y1, P2Y12, and P2X1), which leads to platelet
activation..
Answer: Irreversible step in platelet aggregation? Release of PDF3 Release
of ADP Platelet shape All of the above
◍ Feedback As per AABB Standards, at least 90% of platelet pheresis units
sampled must contain at least 3.0 x 10^11 platelets..
Answer: According to AABB Standards, at least 90% of all Apheresis
Platelets units tested shall contain a minimum of how many platelets? 5.5 X
10^11 5.0 X 10^11 3.0 X 10^11 6.5 X 10^11
◍ D. hh onlyFeedback H antigen deficiency is known as the "Bombay
phenotype" (h/h, also known as Oh) and is found in 1 of 10,000 individuals
in India and 1 in a million people in Europe. There is no ill effect with being
H deficient, but if a blood transfusion is ever needed, people with this blood
type can receive blood only from other donors who are also H deficient..
Answer: Which genotype(s) will give rise to the Bombay phenotype? HH
, only HH and Hh Hh and hh hh only
◍ The best diagnostic test for excessive alcoholic consumption: AST ALP
GGT ALT.
Answer: C. GGTFeedback GGT can be used to screen for chronic alcohol
abuse (it will be elevated in about 75% of chronic drinkers) and to monitor
for alcohol use.
◍ C. diptheriaeFeedback The principal features of the Corynebacterium genus
were described by Collins and Cummins in 1986. They are gram-positive,
catalase-positive, nonspore-forming, nonmotile, rod-shaped bacteria that are
straight or slightly curved Metachromatic granules are usually present
representing stored phosphate regions..
Answer: This catalase positive, gram positive bacilli with diptheroid
morphology is highly resistant to many antibiotics and is associated with
immunocompromised patients. C. jeikeium L. monocytogenes C. diptheriae
E. rhusiopathiae
◍ What is the effect of dextran, why it is difficult to interpret in blood
banking? It inhibits Anti-A and Anti-B It causes rouleaux It destroys the D
antigen on red cell surface It has soluble like antigen.
Answer: B. It causes rouleaux.Feedback Rouleaux- pseudoagglutination or
the false clumping of erythrocytes when the cells are suspended in their own
serum. This phenomenon resembles agglutination and is due to the presence
of an abnormal protein, plasma expanders such as DEXTRAN or wharton's
jelly from cord blood samples.
◍ A. BUNFeedback Azotemia is a medical condition characterized by
abnormally high levels of nitrogen containing compounds..
Answer: Azotemia is increase in? BUN Creatinine Ammonia Uric Acid
◍ A patient with walking pneumonia is being treated with penicillin. State one
possible condition why the patient is not recovering:
A. The organism produces beta lactamase
B. The organism has no cell wall
C. The organism has capsule
, D. The organism produces an enzyme that neutralizes the antibiotic.
Answer: B. The organism has no cell wall. Feedback Pneumonia caused by
Mycoplasma pneumoniae is routinely treated with antibiotics, although the
disease is usually self-limiting. The disease is treated with macrolide,
tetracycline, or fluoroquinolone classes of antibiotics. All mycoplasmas lack
a cell wall and, therefore, all are inherently resistant to beta-lactam
antibiotics (e.g., penicillin).
◍ True.
Answer: True or false: The ACE Exam outline must be validated by
thousands of ACE certified personal trainers before it is approved and made
official
◍ Drug toxicity screening of a 6 year old girl has increased presence of
ACETAMINOPHE
N. What test should be done next? ALT and AST BUN and Creatinine
Salicylates CK and LDH.
Answer: A. ALT and ASTFeedback Aspartate aminotransferase (AST) and
alanine aminotransferase (ALT) concentrations begin to rise within 24 hours
after an acute ingestion and peak at about 72 hours. In severe overdose,
transaminase elevation can be detected as early as 12-16 hours
post-ingestion. Toxicity is defined as serum AST or ALT concentrations
greater than 1000 IU/L. A rapid progression of transaminase values to 3000
IU/L or greater reflects severe hepatotoxicity.
◍ A. Adjust pH to 7.2.
Answer: Standardization of Mueller-Hinton Agar? adjust pH to 7.2 adjust
pH to 6.5 adjust pH to 8.5 All of the above
◍ C. Incubate the culture tubes for 2-3 weeks longerFeedback CMV is a slow
growing herpesvirus, and may require 3 weeks to grow in conventional viral
culture..
Answer: A urine specimen was submitted for isolation of cytomegalovirus
(CMV). The urine was inoculated into human fibroblast tissue culture tubes.
After 72 hours, no cytopathic effect was observed in the culture tubes. The
QUESTIONS WITH ANSWERS GRADED A+
◍ Characteristic: Red Bull's Eye colony in CIN Medium: Klebsiella
pneumoniae Serratia marcescens Clostridium difficile Yersinia
enterocolitica.
Answer: Feedback The characteristic deep red center with a transparent
margin, or "bull's-eye" appearance of Yersinia and Aeromonas colonies is
important for identification, and is due to the presence of mannitol. Y.
enterocolitica ferments the mannitol in the medium, producing an acid pH
which gives the colonies their red color and the "bull's eye" appearance.
◍ D. Chronic Lymphocytic Leukemia (CLL)Feedback Smudge cells are
remnants of cells that lack any identifiable cytoplasmic membrane or
nuclear structure. Smudge cells, also called basket cells, are most often
associated with abnormally fragile lymphocytes in disorders such as chronic
lymphocytic leukemia (CLL)..
Answer: Smudge cells are seen in what type of leukemia? Acute
Myelogenous Leukemia (AML) Chronic Myelogenous Leukemia (CML)
Acute Lymphocytic Leukemia (ALL) Chronic Lymphocytic Leukemia
(CLL)
◍ B. Bordetella pertussisFeedback Stages of Whooping cough: 1. Catarrhal, 2.
Paroxysmal, 3. Convalescence, preferred sample of choice of isolation of
B. pertussis is nasopharyngeal swab..
Answer: "Whooping cough bacillus": Bordetella bronchiseptica Bordetella
pertussis Haemophilus aegypticus NOTA
◍ Indole: negativeMR: negativeLDC: negativeODC: positiveADH: positive
K. pneumoniae
K. oxytoca
, E. cloacae
E. aerogenes.
Answer: C. E cloacae
◍ Rheumatoid Factor:
A. IgG that binds to the Fc portion of abnormal IgM
B. IgM that binds to the Fc portion of abnormal IgG
C. A and B
D. None of the above.
Answer: B. IgM that binds to the Fc portion of abnormal IgG
◍ Minimum competency.
Answer: ACE certification provides the ability to work in the fitness field.
This requirement is referred to as what?
◍ B. Release of ADPFeedback Under normal conditions, small disk-shape
platelets circulate in the blood freely and without interaction with one
another. ADP is stored in dense bodies inside blood platelets and is released
upon platelet activation. ADP interacts with a family of ADP receptors
found on platelets (P2Y1, P2Y12, and P2X1), which leads to platelet
activation..
Answer: Irreversible step in platelet aggregation? Release of PDF3 Release
of ADP Platelet shape All of the above
◍ Feedback As per AABB Standards, at least 90% of platelet pheresis units
sampled must contain at least 3.0 x 10^11 platelets..
Answer: According to AABB Standards, at least 90% of all Apheresis
Platelets units tested shall contain a minimum of how many platelets? 5.5 X
10^11 5.0 X 10^11 3.0 X 10^11 6.5 X 10^11
◍ D. hh onlyFeedback H antigen deficiency is known as the "Bombay
phenotype" (h/h, also known as Oh) and is found in 1 of 10,000 individuals
in India and 1 in a million people in Europe. There is no ill effect with being
H deficient, but if a blood transfusion is ever needed, people with this blood
type can receive blood only from other donors who are also H deficient..
Answer: Which genotype(s) will give rise to the Bombay phenotype? HH
, only HH and Hh Hh and hh hh only
◍ The best diagnostic test for excessive alcoholic consumption: AST ALP
GGT ALT.
Answer: C. GGTFeedback GGT can be used to screen for chronic alcohol
abuse (it will be elevated in about 75% of chronic drinkers) and to monitor
for alcohol use.
◍ C. diptheriaeFeedback The principal features of the Corynebacterium genus
were described by Collins and Cummins in 1986. They are gram-positive,
catalase-positive, nonspore-forming, nonmotile, rod-shaped bacteria that are
straight or slightly curved Metachromatic granules are usually present
representing stored phosphate regions..
Answer: This catalase positive, gram positive bacilli with diptheroid
morphology is highly resistant to many antibiotics and is associated with
immunocompromised patients. C. jeikeium L. monocytogenes C. diptheriae
E. rhusiopathiae
◍ What is the effect of dextran, why it is difficult to interpret in blood
banking? It inhibits Anti-A and Anti-B It causes rouleaux It destroys the D
antigen on red cell surface It has soluble like antigen.
Answer: B. It causes rouleaux.Feedback Rouleaux- pseudoagglutination or
the false clumping of erythrocytes when the cells are suspended in their own
serum. This phenomenon resembles agglutination and is due to the presence
of an abnormal protein, plasma expanders such as DEXTRAN or wharton's
jelly from cord blood samples.
◍ A. BUNFeedback Azotemia is a medical condition characterized by
abnormally high levels of nitrogen containing compounds..
Answer: Azotemia is increase in? BUN Creatinine Ammonia Uric Acid
◍ A patient with walking pneumonia is being treated with penicillin. State one
possible condition why the patient is not recovering:
A. The organism produces beta lactamase
B. The organism has no cell wall
C. The organism has capsule
, D. The organism produces an enzyme that neutralizes the antibiotic.
Answer: B. The organism has no cell wall. Feedback Pneumonia caused by
Mycoplasma pneumoniae is routinely treated with antibiotics, although the
disease is usually self-limiting. The disease is treated with macrolide,
tetracycline, or fluoroquinolone classes of antibiotics. All mycoplasmas lack
a cell wall and, therefore, all are inherently resistant to beta-lactam
antibiotics (e.g., penicillin).
◍ True.
Answer: True or false: The ACE Exam outline must be validated by
thousands of ACE certified personal trainers before it is approved and made
official
◍ Drug toxicity screening of a 6 year old girl has increased presence of
ACETAMINOPHE
N. What test should be done next? ALT and AST BUN and Creatinine
Salicylates CK and LDH.
Answer: A. ALT and ASTFeedback Aspartate aminotransferase (AST) and
alanine aminotransferase (ALT) concentrations begin to rise within 24 hours
after an acute ingestion and peak at about 72 hours. In severe overdose,
transaminase elevation can be detected as early as 12-16 hours
post-ingestion. Toxicity is defined as serum AST or ALT concentrations
greater than 1000 IU/L. A rapid progression of transaminase values to 3000
IU/L or greater reflects severe hepatotoxicity.
◍ A. Adjust pH to 7.2.
Answer: Standardization of Mueller-Hinton Agar? adjust pH to 7.2 adjust
pH to 6.5 adjust pH to 8.5 All of the above
◍ C. Incubate the culture tubes for 2-3 weeks longerFeedback CMV is a slow
growing herpesvirus, and may require 3 weeks to grow in conventional viral
culture..
Answer: A urine specimen was submitted for isolation of cytomegalovirus
(CMV). The urine was inoculated into human fibroblast tissue culture tubes.
After 72 hours, no cytopathic effect was observed in the culture tubes. The
