ACE ASCP COMPREHENSIVE STUDY GUIDE
2026 FULL QUESTIONS AND SOLUTIONS
GRADED A+
◍ Vibrio culture media: Fletcher's Agar Bismuth Sulfite Agar TCBS BAP.
Answer: C. TCBSFeedback Thiosulfate-citrate-bile salts-sucrose agar, or
TCBS agar, is a type of selective agar culture plate that is used in
microbiology laboratories to isolate Vibrio spp.
◍ A patient's record shows a previous anti-Jk(b), but the current antibody
screen is negative. What further testing should be done before transfusion?
A. Give Jk(b) negative crossmatch compatible blood
B. Crossmatch type specific units and release only compatible units for
transfusion
C. Phenotype the patient's red cells for the Jk(b) antigen
D. AOTA.
Answer: A. Give Jk(b) negative crossmatch compatible bloodFeedback
Delayed hemolytic transfusion reactions may occur in recipients who are
previously immunized but who do not have detectable antibody, if they
receive blood with the corresponding antigen. When there is a history of
clinically significant antibodies, donor red cells should be phenotyped and
antigen-negative blood selected. A complete AHG crossmatch must be
performed.
◍ D. Delay in set upFeedback FALSE INCREASED RATE: Tube tilted (not
vertical), Vibration of tube during test, Test > 1hr, Improper blood dilution,
Improper mixing of blood and room temperature >25
C. FALSE DECREASED RATE: Low temperature of blood, air bubbles in
tube, Test <1 hr, Improper blood dilution, improper mixing of blood, room
temperature <20
, C. .
Answer: Which of the following can cause false DECREASE in ESR?
Macrocytes Tilting of tube Vibration Delay in set up
◍ B. Hemoglobin FFeedback The test is based on differences between
maternal and fetal hemoglobin. Maternal blood contains adult hemoglobin
composed of two alpha and two beta subunits (aka hemoglobin A or HbA;
i.e., normal adult hemoglobin). Fetal blood contains fetal hemoglobin
composed of two alpha and two gamma subunits (aka hemoglobin F or HbF;
i.e., normal fetal hemoglobin)..
Answer: Alkali denaturation test detects what type of hemoglobin?
Hemoglobin C Hemoglobin F Hemoglobin S Hemoglobin H
◍ Which of the following drugs is used to treat bipolar disorders (manic
depression)? Vancomycin Digoxin Lithium Procainamide.
Answer: C. LithiumFeedback Lithium compounds, also known as lithium
salts, are primarily used as a psychiatric medication. This includes in the
treatment of major depressive disorder that does not improve following the
use of other antidepressants, and bipolar disorder. In these disorders, it
reduces the risk of suicide.
◍ D. A and bFeedback Schüffner's dots refers to a hematological finding that
is associated with malaria, exclusively found in Plasmodium ovale and
Plasmodium vivax. Plasmodium vivax induces morphologic alterations in
infected host erythrocytes that are visible by light microscopy in
Romanowsky-stained blood smears as multiple brick-red dots. These
morphologic changes, referred to as Schüffner's dots, are important in the
identification of this species of malarial parasite and have been associated
by electron microscopy with caveolavesicle complexes along the
erythrocyte plasmalemma. ..
Answer: Which of the following Plasmodium species will you see Schuffner
dots? P. vivax P. ovale P. malariae A and B
◍ Urinalysis result shows the following:RBC= 20-25/hpfHyaline cast-
6-8/hpfWBC= 5-10/hpfRTE Cells: 25-35/hpfThey are most likely indicative
, of:
A. Glomerulonephritis
B. Tubular necrosis
C. Pyelonephritis
D. Nephrotic syndrome.
Answer: B. Tubular necrosis
◍ A. Ankylosing spondylitisFeedback:Ankylosing spondylitis belongs to a
group of arthritis conditions that tend to cause chronic inflammation of the
spine (spondyloarthropathies). The HLA-B27 gene can be detected in the
blood of most patients with ankylosing spondylitis..
Answer: What disease is associated with the possession of HLA-B27?
Ankylosing spondylitis Hashimoto's Disease Goodpasture's syndrome SLE
◍ Autologous Donation: Age has no requirement Hgb atleast 11 g/dl, Hct
atleast 33% Single unit is removed at a time, with atleast 3 day intervals
AOTA.
Answer: D. AOTAFeedback Donating blood for your own surgery is called
an autologous donation. These blood units are collected and stored until the
patient's surgery.
◍ B. HDL, LDL, IDL, VLDL.
Answer: The descending arrangement of lipoproteins according to
protein/lipid ratio: Chylomicron,IDL,VLDL,LDL,HDL HDL, LDL, IDL,
VLDL Chylomicron, VLDL, IDL,LDL None of the above
◍ Which of the following methods used for HIV identification is considered a
signal amplification technique? DNA PCR Reverse Transcriptase PCR
Branched Chain DNA Analysis Nucleic acid sequence-based assay
(NASBA).
Answer: C. Branched Chain DNA analysis.Feedback Branched DNA
(bDNA) is a signal amplification technology used in clinical and research
laboratories to quantitatively detect nucleic acids. An overnight incubation
is a significant drawback of highly sensitive bDNA assays.
◍ D. Heinz bodiesFeedback Ingestion of naphthalene-containing mothballs is
, a common occurrence in children. Heinz body formation, hemoglobinuria
and mild methemoglobinaemia can occur due to poisoning with this
substance.
Answer: Moth balls (naphtalene) poisoning may show which of the
following red cell characteristics? Howell-Jolly bodies Ovalocytes Sickle
cells Heinz Bodies
◍ Urine with an specific gravity consistently between 1.002 and 1.003
indicates:
A. Acute glomerulonephritis
B. Renal tubular failure
C. Addison's disease
D. Diabetes insipidus.
Answer: D. Diabetes insipidusFeedback Low specific gravity (SG)
(1.001-1.003) may indicate the presence of diabetes insipidus, a disease
caused by impaired functioning of antidiuretic hormone (ADH). Low SG
also may occur in patients with glomerulonephritis, pyelonephritis, and
other renal abnormalities.
◍ An antiepileptic (or anticonvulsant) used to control seizure disorders is:
Acetaminophen Lithium Phenytoin Digoxin.
Answer: C. PhenytoinFeedback Phenytoin is an anti-epileptic drug, also
called an anticonvulsant. It works by slowing down impulses in the brain
that cause seizures. Phenytoin is used to control seizures
◍ The manual eosinophil count by hemocytometer is also called:
A. Thorn test
B. Direct absolute eosinophil count
C. Relative eosinophil count
D. Indirect absolute eosinophil count.
Answer: B. Direct absolute eosinophil countFeedback It is possible to get
direct eosinophil count. The direct method is quick and gives exact value of
eosinophil. In this method the blood is diluted to 1:10 with a special diluting
fluid. The diluted blood specimen is then charged in the counting chamber
2026 FULL QUESTIONS AND SOLUTIONS
GRADED A+
◍ Vibrio culture media: Fletcher's Agar Bismuth Sulfite Agar TCBS BAP.
Answer: C. TCBSFeedback Thiosulfate-citrate-bile salts-sucrose agar, or
TCBS agar, is a type of selective agar culture plate that is used in
microbiology laboratories to isolate Vibrio spp.
◍ A patient's record shows a previous anti-Jk(b), but the current antibody
screen is negative. What further testing should be done before transfusion?
A. Give Jk(b) negative crossmatch compatible blood
B. Crossmatch type specific units and release only compatible units for
transfusion
C. Phenotype the patient's red cells for the Jk(b) antigen
D. AOTA.
Answer: A. Give Jk(b) negative crossmatch compatible bloodFeedback
Delayed hemolytic transfusion reactions may occur in recipients who are
previously immunized but who do not have detectable antibody, if they
receive blood with the corresponding antigen. When there is a history of
clinically significant antibodies, donor red cells should be phenotyped and
antigen-negative blood selected. A complete AHG crossmatch must be
performed.
◍ D. Delay in set upFeedback FALSE INCREASED RATE: Tube tilted (not
vertical), Vibration of tube during test, Test > 1hr, Improper blood dilution,
Improper mixing of blood and room temperature >25
C. FALSE DECREASED RATE: Low temperature of blood, air bubbles in
tube, Test <1 hr, Improper blood dilution, improper mixing of blood, room
temperature <20
, C. .
Answer: Which of the following can cause false DECREASE in ESR?
Macrocytes Tilting of tube Vibration Delay in set up
◍ B. Hemoglobin FFeedback The test is based on differences between
maternal and fetal hemoglobin. Maternal blood contains adult hemoglobin
composed of two alpha and two beta subunits (aka hemoglobin A or HbA;
i.e., normal adult hemoglobin). Fetal blood contains fetal hemoglobin
composed of two alpha and two gamma subunits (aka hemoglobin F or HbF;
i.e., normal fetal hemoglobin)..
Answer: Alkali denaturation test detects what type of hemoglobin?
Hemoglobin C Hemoglobin F Hemoglobin S Hemoglobin H
◍ Which of the following drugs is used to treat bipolar disorders (manic
depression)? Vancomycin Digoxin Lithium Procainamide.
Answer: C. LithiumFeedback Lithium compounds, also known as lithium
salts, are primarily used as a psychiatric medication. This includes in the
treatment of major depressive disorder that does not improve following the
use of other antidepressants, and bipolar disorder. In these disorders, it
reduces the risk of suicide.
◍ D. A and bFeedback Schüffner's dots refers to a hematological finding that
is associated with malaria, exclusively found in Plasmodium ovale and
Plasmodium vivax. Plasmodium vivax induces morphologic alterations in
infected host erythrocytes that are visible by light microscopy in
Romanowsky-stained blood smears as multiple brick-red dots. These
morphologic changes, referred to as Schüffner's dots, are important in the
identification of this species of malarial parasite and have been associated
by electron microscopy with caveolavesicle complexes along the
erythrocyte plasmalemma. ..
Answer: Which of the following Plasmodium species will you see Schuffner
dots? P. vivax P. ovale P. malariae A and B
◍ Urinalysis result shows the following:RBC= 20-25/hpfHyaline cast-
6-8/hpfWBC= 5-10/hpfRTE Cells: 25-35/hpfThey are most likely indicative
, of:
A. Glomerulonephritis
B. Tubular necrosis
C. Pyelonephritis
D. Nephrotic syndrome.
Answer: B. Tubular necrosis
◍ A. Ankylosing spondylitisFeedback:Ankylosing spondylitis belongs to a
group of arthritis conditions that tend to cause chronic inflammation of the
spine (spondyloarthropathies). The HLA-B27 gene can be detected in the
blood of most patients with ankylosing spondylitis..
Answer: What disease is associated with the possession of HLA-B27?
Ankylosing spondylitis Hashimoto's Disease Goodpasture's syndrome SLE
◍ Autologous Donation: Age has no requirement Hgb atleast 11 g/dl, Hct
atleast 33% Single unit is removed at a time, with atleast 3 day intervals
AOTA.
Answer: D. AOTAFeedback Donating blood for your own surgery is called
an autologous donation. These blood units are collected and stored until the
patient's surgery.
◍ B. HDL, LDL, IDL, VLDL.
Answer: The descending arrangement of lipoproteins according to
protein/lipid ratio: Chylomicron,IDL,VLDL,LDL,HDL HDL, LDL, IDL,
VLDL Chylomicron, VLDL, IDL,LDL None of the above
◍ Which of the following methods used for HIV identification is considered a
signal amplification technique? DNA PCR Reverse Transcriptase PCR
Branched Chain DNA Analysis Nucleic acid sequence-based assay
(NASBA).
Answer: C. Branched Chain DNA analysis.Feedback Branched DNA
(bDNA) is a signal amplification technology used in clinical and research
laboratories to quantitatively detect nucleic acids. An overnight incubation
is a significant drawback of highly sensitive bDNA assays.
◍ D. Heinz bodiesFeedback Ingestion of naphthalene-containing mothballs is
, a common occurrence in children. Heinz body formation, hemoglobinuria
and mild methemoglobinaemia can occur due to poisoning with this
substance.
Answer: Moth balls (naphtalene) poisoning may show which of the
following red cell characteristics? Howell-Jolly bodies Ovalocytes Sickle
cells Heinz Bodies
◍ Urine with an specific gravity consistently between 1.002 and 1.003
indicates:
A. Acute glomerulonephritis
B. Renal tubular failure
C. Addison's disease
D. Diabetes insipidus.
Answer: D. Diabetes insipidusFeedback Low specific gravity (SG)
(1.001-1.003) may indicate the presence of diabetes insipidus, a disease
caused by impaired functioning of antidiuretic hormone (ADH). Low SG
also may occur in patients with glomerulonephritis, pyelonephritis, and
other renal abnormalities.
◍ An antiepileptic (or anticonvulsant) used to control seizure disorders is:
Acetaminophen Lithium Phenytoin Digoxin.
Answer: C. PhenytoinFeedback Phenytoin is an anti-epileptic drug, also
called an anticonvulsant. It works by slowing down impulses in the brain
that cause seizures. Phenytoin is used to control seizures
◍ The manual eosinophil count by hemocytometer is also called:
A. Thorn test
B. Direct absolute eosinophil count
C. Relative eosinophil count
D. Indirect absolute eosinophil count.
Answer: B. Direct absolute eosinophil countFeedback It is possible to get
direct eosinophil count. The direct method is quick and gives exact value of
eosinophil. In this method the blood is diluted to 1:10 with a special diluting
fluid. The diluted blood specimen is then charged in the counting chamber
