ACE ASCP PRACTICE EXAMINATION 2026
QUESTIONS WITH ANSWERS GRADED A+
◍ 17-Ketosteroids is produced in the
A. Adrenal glands
B. Hypothalamus
C. Thyroid gland
D. Pituitary gland.
Answer: A. Adrenal glands17-ketosteroids are substances that form when
the body breaks down male steroid sex hormones called androgens and other
hormones released by the adrenal glands in males and females, and by the
testes in males
◍ Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC
ACID CONTAMINATIO
N. A. True
B. False.
Answer: A. True
◍ B. 6.09Feedback:CV= SD/meanX100, (5.48/90)X100= 6.09..
Answer: What is the coefficient of variation if the mean is 90 and the
standard deviation is 5.48? 2.75 6.09 5.68 30
◍ Which of the following causes antibody against TSH?SLEGrave's Disease
Hashimoto's ThyroiditisRheumatoid Arthritis.
Answer: B. Grave's diseaseFeedback The thyrotropin receptor (TSH
receptor) is the antigen for TSH receptor antibodies (TRAbs). It is a seven
transmembrane G protein coupled receptor that is involved in thyroid
hormone signalling. TRAbs are present in 70-100% of Grave's Disease. In
Hashimoto's Thyroiditis, T4 is decreased and TSH is increased. Anti-TPO
(anti-thyroid peroxidase) antibodies are most commonly associated with
, Hashimoto's thyroiditis.
◍ Which of the following blood test uses radioimmunoassay test to detect
direct and specific IgE? Radioallergosorbent Test (RAST)
Radioimmunosorbent Test (RIST) A and B None of the above.
Answer: A. Radioallergosorbent Test (RAST)Feedback RAST test is used to
detect direct and specific IgE. RIST test is used to detect total serum IgE.
◍ According to AABB standards, Fresh Frozen Plasma (FFP) must be infused
within what period of time following thawing? 24 hrs 36 hrs 48 hrs 72 hrs.
Answer: A. 24 hours
◍ First marker to be positive in HIV infection: HIV antibody HIV RNA
CD4/CD8 ratio p24.
Answer: D. p24Feedback The p24 antigen test can detect the p24 protein on
average 10 to 14 days after infection with HIV. p24 antigen is a viral protein
that makes up most of the viral core. Serum concentrations of p24 antigen
are high in the first few weeks after infection; tests sensitive to p24 antigen
are therefore useful for diagnosing very early infection when antibody levels
are still low.
◍ C. 3 days.
Answer: Maximum interval which a recipient sample maybe used for
crossmatching if the patient has been recently transfused, has been
pregnant? 2 days 6 days 3 days NOTA
◍ S. pyogenesFeedback
S. pyogenes is the most common causative agent of pharyngitis..
Answer: A patient was diagnosed with streptococcal pharyngitis 6 months
ago. Now he is showing signs of glumerulonephritis. What will be seen in
the renal biopsy? S. agalactiae S. pyogenes H. influenzae S. aureusNext
◍ Which of the following Plasmodium would least likely show the presence of
trophozoites and schizonts in the peripheral blood?
P. malariae
P. vivax
, P. falciparum
P. ovale.
Answer: P. FalciparumFeedback It is unusual to see mature trophozoites or
schizonts of P. falciparum in the peripheral blood smears, as these are
usually sequestered in the tissues
◍ Presence of lupus anticoagulant leads to: Activation of fibrinolytic system
Thrombosis (micro emboli clots) Bleeding Continuous activation of the
clotting cascade.
Answer: B. Thrombosis (micro emboli clots)Feedback Lupus anticoagulant
is a misnomer, as it is actually a prothrombotic agent. Lupus anticoagulant
antibodies in living systems cause an increase in inappropriate blood
clotting.
◍ Cause of false negative ABO typing: Rouleaux Positive DAT 37C LISS.
Answer: B. Positive DATFeedback A positive DAT means that there are
antibodies attached to the RBCs, thus giving false negative results.
◍ A. Acute lymphocytic leukemiaFeedback Terminal deoxynucleotidyl
transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or
terminal transferase, is a specialized DNA polymerase expressed in
immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic
leukemia/lymphoma cells..
Answer: Increased levels of TdT (Terminal deoxynucleotidyl transferase)
activity are indicative of: Acute lymphocytic leukemia Acute myelocytic
leukemia Burkitt lymphoma Eosinophilia
◍ What is measured in the urease method determination of BUN?
A. Uricase
B. Urea
C. NAD
D. Nitrogen.
Answer: C. NADFeedback Nicotinamide Adenine Dinucleotide (NAD) is
being measured in the final step of urease method determination of BUN, it
is measured at 340nm.
, ◍ Final degenerative form of cast
A. Waxy cast
B. Hyaline cast
C. WBC cast
D. RBC cast.
Answer: A. Waxy cast
◍ A. D>c>E>C>eFeedback Most immunogenic D > c > E > C > e least
immunogenic..
Answer: Arrange the common Rh antigens according to immunogenicity
(greatest to least): D>c>E>C>e D>C>E>c>e D>c>E>e>C C>D>c>E>e
◍ The following results were obtained from a pure culture of gram negative
rods recovered from the pulmonary secretions of a 10 year old cystic
fibrosis patient with pneumonia: Oxidase (+), Motility (+), Growth at 42C
(+), Pigment= Red (Nonflouresecent) Pseudomonas aeruginosa
Burkholderia cepacia Burkholderia pseudomallei Pseudomonas stutzeri.
Answer: A. Pseudomonas aeruginosaFeedback P. aeruginosa is growing as
biofilm within the lungs of cystic fibrosis patients. In certain conditions, P.
aeruginosa can secrete a variety of pigments, including pyocyanin
(blue-green), pyoverdine (yellow-green and fluorescent), and pyorubin
(red-brown). These can be used to identify the organism.
◍ Primidone monitoring: suspected to have primidone overdose, but the serum
concentration is within the normal range, what should the MLS do next?
A. Measure procainamide concentration
B. Measure phenobarbital concentration
C. Measure phenytoin concentration
D. Eliminate primidone overdose as possible diagnosis.
Answer: B. Measure phenobarbital concentrationFeedback Primidone is a
structural analog of phenobarbital.
◍ CFeedback In the "pattern of non-identity", none of the antibodies in the
antiserum react with antigenic determinants that may be present in both the
antigens, i.e., the two antigens are immunologically unrelated as far as that
QUESTIONS WITH ANSWERS GRADED A+
◍ 17-Ketosteroids is produced in the
A. Adrenal glands
B. Hypothalamus
C. Thyroid gland
D. Pituitary gland.
Answer: A. Adrenal glands17-ketosteroids are substances that form when
the body breaks down male steroid sex hormones called androgens and other
hormones released by the adrenal glands in males and females, and by the
testes in males
◍ Most common error in Polymerase Chain Reaction (PCR) is NUCLEIC
ACID CONTAMINATIO
N. A. True
B. False.
Answer: A. True
◍ B. 6.09Feedback:CV= SD/meanX100, (5.48/90)X100= 6.09..
Answer: What is the coefficient of variation if the mean is 90 and the
standard deviation is 5.48? 2.75 6.09 5.68 30
◍ Which of the following causes antibody against TSH?SLEGrave's Disease
Hashimoto's ThyroiditisRheumatoid Arthritis.
Answer: B. Grave's diseaseFeedback The thyrotropin receptor (TSH
receptor) is the antigen for TSH receptor antibodies (TRAbs). It is a seven
transmembrane G protein coupled receptor that is involved in thyroid
hormone signalling. TRAbs are present in 70-100% of Grave's Disease. In
Hashimoto's Thyroiditis, T4 is decreased and TSH is increased. Anti-TPO
(anti-thyroid peroxidase) antibodies are most commonly associated with
, Hashimoto's thyroiditis.
◍ Which of the following blood test uses radioimmunoassay test to detect
direct and specific IgE? Radioallergosorbent Test (RAST)
Radioimmunosorbent Test (RIST) A and B None of the above.
Answer: A. Radioallergosorbent Test (RAST)Feedback RAST test is used to
detect direct and specific IgE. RIST test is used to detect total serum IgE.
◍ According to AABB standards, Fresh Frozen Plasma (FFP) must be infused
within what period of time following thawing? 24 hrs 36 hrs 48 hrs 72 hrs.
Answer: A. 24 hours
◍ First marker to be positive in HIV infection: HIV antibody HIV RNA
CD4/CD8 ratio p24.
Answer: D. p24Feedback The p24 antigen test can detect the p24 protein on
average 10 to 14 days after infection with HIV. p24 antigen is a viral protein
that makes up most of the viral core. Serum concentrations of p24 antigen
are high in the first few weeks after infection; tests sensitive to p24 antigen
are therefore useful for diagnosing very early infection when antibody levels
are still low.
◍ C. 3 days.
Answer: Maximum interval which a recipient sample maybe used for
crossmatching if the patient has been recently transfused, has been
pregnant? 2 days 6 days 3 days NOTA
◍ S. pyogenesFeedback
S. pyogenes is the most common causative agent of pharyngitis..
Answer: A patient was diagnosed with streptococcal pharyngitis 6 months
ago. Now he is showing signs of glumerulonephritis. What will be seen in
the renal biopsy? S. agalactiae S. pyogenes H. influenzae S. aureusNext
◍ Which of the following Plasmodium would least likely show the presence of
trophozoites and schizonts in the peripheral blood?
P. malariae
P. vivax
, P. falciparum
P. ovale.
Answer: P. FalciparumFeedback It is unusual to see mature trophozoites or
schizonts of P. falciparum in the peripheral blood smears, as these are
usually sequestered in the tissues
◍ Presence of lupus anticoagulant leads to: Activation of fibrinolytic system
Thrombosis (micro emboli clots) Bleeding Continuous activation of the
clotting cascade.
Answer: B. Thrombosis (micro emboli clots)Feedback Lupus anticoagulant
is a misnomer, as it is actually a prothrombotic agent. Lupus anticoagulant
antibodies in living systems cause an increase in inappropriate blood
clotting.
◍ Cause of false negative ABO typing: Rouleaux Positive DAT 37C LISS.
Answer: B. Positive DATFeedback A positive DAT means that there are
antibodies attached to the RBCs, thus giving false negative results.
◍ A. Acute lymphocytic leukemiaFeedback Terminal deoxynucleotidyl
transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or
terminal transferase, is a specialized DNA polymerase expressed in
immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic
leukemia/lymphoma cells..
Answer: Increased levels of TdT (Terminal deoxynucleotidyl transferase)
activity are indicative of: Acute lymphocytic leukemia Acute myelocytic
leukemia Burkitt lymphoma Eosinophilia
◍ What is measured in the urease method determination of BUN?
A. Uricase
B. Urea
C. NAD
D. Nitrogen.
Answer: C. NADFeedback Nicotinamide Adenine Dinucleotide (NAD) is
being measured in the final step of urease method determination of BUN, it
is measured at 340nm.
, ◍ Final degenerative form of cast
A. Waxy cast
B. Hyaline cast
C. WBC cast
D. RBC cast.
Answer: A. Waxy cast
◍ A. D>c>E>C>eFeedback Most immunogenic D > c > E > C > e least
immunogenic..
Answer: Arrange the common Rh antigens according to immunogenicity
(greatest to least): D>c>E>C>e D>C>E>c>e D>c>E>e>C C>D>c>E>e
◍ The following results were obtained from a pure culture of gram negative
rods recovered from the pulmonary secretions of a 10 year old cystic
fibrosis patient with pneumonia: Oxidase (+), Motility (+), Growth at 42C
(+), Pigment= Red (Nonflouresecent) Pseudomonas aeruginosa
Burkholderia cepacia Burkholderia pseudomallei Pseudomonas stutzeri.
Answer: A. Pseudomonas aeruginosaFeedback P. aeruginosa is growing as
biofilm within the lungs of cystic fibrosis patients. In certain conditions, P.
aeruginosa can secrete a variety of pigments, including pyocyanin
(blue-green), pyoverdine (yellow-green and fluorescent), and pyorubin
(red-brown). These can be used to identify the organism.
◍ Primidone monitoring: suspected to have primidone overdose, but the serum
concentration is within the normal range, what should the MLS do next?
A. Measure procainamide concentration
B. Measure phenobarbital concentration
C. Measure phenytoin concentration
D. Eliminate primidone overdose as possible diagnosis.
Answer: B. Measure phenobarbital concentrationFeedback Primidone is a
structural analog of phenobarbital.
◍ CFeedback In the "pattern of non-identity", none of the antibodies in the
antiserum react with antigenic determinants that may be present in both the
antigens, i.e., the two antigens are immunologically unrelated as far as that
