BIO SCI M118L (MICROBIO LAB) QUIZ
1 QUESTIONS WITH CORRECT
ANSWERS
\.Where do you dispose contaminated items? - ANSWERS✔-biohazard containers
\.Where do you dispose broken glass? - ANSWERS✔-disposable glass containers
\.Where do you place non-disposable items? - ANSWERS✔-dirty dish carts
\.What sources do bacteria require to survive? - ANSWERS✔-carbon and nitrogen
(possibly amino acids, vitamins, and other growth factors)
\.defined media - ANSWERS✔-all components have been characterized and are
known
\.complex media - ANSWERS✔-contains one or more ingredients of unknown
composition
\.peptones - ANSWERS✔-unknown components; nutrient rich extracts from milk,
yeast, beef, etc.
, \.selective media - ANSWERS✔-inhibits growth of a group of organisms
\.example of selective media - ANSWERS✔-MacConkey agar: contains bile salts
that destroy the cell walls of gram positive bacteria, inhibiting their growth -> only
gram negative bacteria can grow
\.enriched media - ANSWERS✔-contains ingredients that promote the growth of
fastidious organisms
\.fastidious organisms - ANSWERS✔-organisms that only grow when there are
certain nutrients available
\.example of enriched media - ANSWERS✔-chocolate agar: contains red blood
cells lysed with heat -> permits growth of organisms that require growth factors
found inside red blood cells
\.differential media - ANSWERS✔-allows one to distinguish between different
types of bacteria growing on a single plate
- colored dyes taken up by specific organisms
- pH indicators that alter the color of media surrounding a colony depending on
the secretion
\.example of differential media - ANSWERS✔-MacConkey agar: contains pH
indicator neutral red
- organisms that ferment lactose (sugar in the media) produce an acid end product
-> lowers pH -> turns red
1 QUESTIONS WITH CORRECT
ANSWERS
\.Where do you dispose contaminated items? - ANSWERS✔-biohazard containers
\.Where do you dispose broken glass? - ANSWERS✔-disposable glass containers
\.Where do you place non-disposable items? - ANSWERS✔-dirty dish carts
\.What sources do bacteria require to survive? - ANSWERS✔-carbon and nitrogen
(possibly amino acids, vitamins, and other growth factors)
\.defined media - ANSWERS✔-all components have been characterized and are
known
\.complex media - ANSWERS✔-contains one or more ingredients of unknown
composition
\.peptones - ANSWERS✔-unknown components; nutrient rich extracts from milk,
yeast, beef, etc.
, \.selective media - ANSWERS✔-inhibits growth of a group of organisms
\.example of selective media - ANSWERS✔-MacConkey agar: contains bile salts
that destroy the cell walls of gram positive bacteria, inhibiting their growth -> only
gram negative bacteria can grow
\.enriched media - ANSWERS✔-contains ingredients that promote the growth of
fastidious organisms
\.fastidious organisms - ANSWERS✔-organisms that only grow when there are
certain nutrients available
\.example of enriched media - ANSWERS✔-chocolate agar: contains red blood
cells lysed with heat -> permits growth of organisms that require growth factors
found inside red blood cells
\.differential media - ANSWERS✔-allows one to distinguish between different
types of bacteria growing on a single plate
- colored dyes taken up by specific organisms
- pH indicators that alter the color of media surrounding a colony depending on
the secretion
\.example of differential media - ANSWERS✔-MacConkey agar: contains pH
indicator neutral red
- organisms that ferment lactose (sugar in the media) produce an acid end product
-> lowers pH -> turns red
