QUESTIONS AND VERIFIED
CORRECT ANSWERS
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EN1 quiz- what nitrogen compound is used as a substrate for denitrification? - CORRECT
ANSWER-nitrate (NO3-)
EN1 quiz- for what metabolic purpose is nitrate used in denitrification? - CORRECT ANSWER-
electron acceptor for anaerobic respiration
EN1 quiz- what is the waste product of denitrification - CORRECT ANSWER-nitrogen (n2)
P1&P3 quiz- what is the targeted % survival for mutagenesis - CORRECT ANSWER-.1-1%
P1&P3 quiz- for UV mutagenesis of Serratia, order steps of procedure from beginning to end
UV exposure
Plate on PG medium
Liquid culture
Serial dilutions - CORRECT ANSWER-1. liquid culture
2. serial dilutions
3. plate on pg medium
4. UV exposure
, P1&P3 quiz- how many unknown species will you receive to identify in P1 - CORRECT ANSWER-2
P1&P3 quiz- what is the first operation to perform when receiving P1 unknowns? - CORRECT
ANSWER-streak on solid media
GE 11 quiz- Which medium (EMB or R2A) corresponds to each condition listed below for
membrane filter methods to count bacteria?
incubated at room temp
incubated at 37*
promotes growth of slow-growing heterotrophs
inhibits gram-positive species - CORRECT ANSWER-incubated at room temp: R2A
incubated at 37*: EMB
promotes growth of slow-growing heterotrophs: R2A
inhibits gram-positive species: EMB
GE 11 quiz- Name 3 characteristics of coliform bacteria - CORRECT ANSWER-ferments lactose
with acid and gas formation
rod-shaped
facultatively anaerobic
GE 11 quiz- once a sample has been filtered, filter is placed in which orientation on the agar? -
CORRECT ANSWER-bacteria up
P3 quiz- What should the P3 complementation assay streak look like - CORRECT ANSWER-Two
parallel upside down L's