REVISION HANDBOOK 2026 ENZYME
FUNCTION AND REGULATION
◉ In total, how many ATP and/or NADH are produced in the glucose
to pyruvate steps in glycolysis? Answer: 2 ATP and 2 NADPH
produced
◉ In converting glucose to pyruvate, how many ATP or NADH are
produced (or required) in the fructose-6-phosphate to fructose-1,6-
bisphosphate step in glycolysis? Answer: 1 ATP required
◉ In converting glucose to pyruvate, how many ATP or NADH are
produced (or required) in the glucose to glucose-6-phosphate step
in glycolysis? Answer: 1 ATP required
◉ In converting glucose to pyruvate, how many ATP or NADH are
produced (or required) in the
glyceraldehyde 3-phosephate to 1,3-bisphosphoglycerate step in
glycolysis? Answer: 2 NADH produced
◉ In converting glucose to pyruvate, how many ATP or NADH are
produced (or required) in the
, 1,3-bisphosphoglycerate to 3-phosphoglycerate step in glycolysis?
Answer: 2 ATP produced
◉ In converting glucose to pyruvate, how many ATP or NADH are
produced (or required) in the
phosphoenolpyruvate to pyruvate step in glycolysis? Answer: 2 ATP
produced
◉ What is the primary product of glycolysis? Answer: pyruvate
◉ In glycolysis, when glucose enters a cell, it is immediately
phosphorylated to form glucose-6-phosphate. The phosphate donor
in this reaction is ATP, and the enzyme is ___. Answer: hexokinase
◉ In glycolysis, when glucose enters a cell, it is immediately
phosphorylated to form glucose-6- phosphate.
The phosphate donor in this reaction is _______, and the enzyme is
hexokinase. Answer: ATP
◉ In glycolysis, when fructose-6-phosphate is preparing to split the
phosphate donor in this reaction is ATP, and the enzyme is ________.
Answer: phosphofructokinase-1