Rhabdoviridae--genome type and envelope?
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- sense RNA, enveloped
,RNA INTERFERANCE (RNAi)
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Antiviral mechanism in plants, mammals, worms, fungi and arthropods.
Triggered by Double stranded RNA
Being used to target viral replication and gene expression
If a virus has glycosylations these are added in...?
A. The lumen of the Endoplasmic reticulum
B. The Golgi apparatus
C. The ribosome
D. The nucleus
E. A or B
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B. The Golgi apparatus
Retroviridae--genome type and envelope?
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+ sense RNA, enveloped
,Assembly of Virion Sites for Enveloped Viruses
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Plasma Membrane [ex: Retro, Rhabdo]
Internal Membranes [ex: Corona, Bunya]
Nuclear Membrane [ex: Herpes]
You use a MOI of 0.1 to infect cells with poliovirus. What are your expectations
a. All the cells will be infected with at least 1 virus particle
b. Only 50% of the cells will be infected
c. You will be able to set up a one-step growth curve
d. This will not result in synchronous infection of the cells
e. A and C are correct answers
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c. You will be able to set up a one-step growth curve
Equation for PFU/mL:
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, PFU/ml=(average number of plaques)x(reciprocal of the
dilution)x(reciprocal of volume)
SYBR Green (Real-Time PCR)
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Dye selectively binds dsDNA
Advantages:
-sequence-independent
-cheaper
Disadvantages:
-specificity-binds to any dsDNA; so if primers misprime, non-template DNA
can be amplified
--false (+)
Following replication at the site of entry, virus particles can remain localized or can
spread to other
tissues in the host.
List routes of dissemination (5).
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1. Local spread on epithelial surfaces
2. Viremia (presence of virus in the blood)
3. Lymphatic system
4. Infection of other organs
Give this one a try later!
- sense RNA, enveloped
,RNA INTERFERANCE (RNAi)
Give this one a try later!
Antiviral mechanism in plants, mammals, worms, fungi and arthropods.
Triggered by Double stranded RNA
Being used to target viral replication and gene expression
If a virus has glycosylations these are added in...?
A. The lumen of the Endoplasmic reticulum
B. The Golgi apparatus
C. The ribosome
D. The nucleus
E. A or B
Give this one a try later!
B. The Golgi apparatus
Retroviridae--genome type and envelope?
Give this one a try later!
+ sense RNA, enveloped
,Assembly of Virion Sites for Enveloped Viruses
Give this one a try later!
Plasma Membrane [ex: Retro, Rhabdo]
Internal Membranes [ex: Corona, Bunya]
Nuclear Membrane [ex: Herpes]
You use a MOI of 0.1 to infect cells with poliovirus. What are your expectations
a. All the cells will be infected with at least 1 virus particle
b. Only 50% of the cells will be infected
c. You will be able to set up a one-step growth curve
d. This will not result in synchronous infection of the cells
e. A and C are correct answers
Give this one a try later!
c. You will be able to set up a one-step growth curve
Equation for PFU/mL:
Give this one a try later!
, PFU/ml=(average number of plaques)x(reciprocal of the
dilution)x(reciprocal of volume)
SYBR Green (Real-Time PCR)
Give this one a try later!
Dye selectively binds dsDNA
Advantages:
-sequence-independent
-cheaper
Disadvantages:
-specificity-binds to any dsDNA; so if primers misprime, non-template DNA
can be amplified
--false (+)
Following replication at the site of entry, virus particles can remain localized or can
spread to other
tissues in the host.
List routes of dissemination (5).
Give this one a try later!
1. Local spread on epithelial surfaces
2. Viremia (presence of virus in the blood)
3. Lymphatic system
4. Infection of other organs
