ACS Biochemistry Exam Questions and Answers –
Comprehensive Biochemistry Study Guide 2026/ 2027
with solution (GRADED A+)
In setting up an assay for measuring the total amount of an enzyme
present in an unknown sample, the substrate concentration should
preferably be: - VERIFIED ANSWER - 10 fold higher than Km
Dialysis would be most useful for the separation of: - VERIFIED ANSWER - A
ammonium sulfate from an enzyme
An inhibitor that increases Km and does not alter the V max value is -
VERIFIED ANSWER - A competitive inhibitor
Which amino acid side chain would not be expected would not be expected
to participate in covalent enzyme catalysis - VERIFIED ANSWER - Leu
Which statement is correct concerning the T(deoxy) and R(oxy) states of
tetrameric hemoglobin - VERIFIED ANSWER - In the T to R transition, the
central cavity of the tetramer decreases in size, preventing the binding of
the allosteric modulator 2,3,-biophosphylglycerate
,When the energy needs of the cell are satisfied, acetyl-CoA accumulates
and serves as a positive allosteric effector for - VERIFIED ANSWER -
pyruvate carboxylase
To determine the Km value for an enzyme reaction, which parameter is
normally varied, while keeping the others constant - VERIFIED ANSWER -
substrate concentration
In a yeast cell growing aerobically on glucose, which would provide most
of the energy for growth? - VERIFIED ANSWER - glycolysis to TCA cycle
and oxidative phosphorylation
Cleavage of disulfide bonds in globular proteins may be brought about by -
VERIFIED ANSWER - Reaction with excess B-mercaptoethanol at pH 8 in 6
M urea
The alpha helical secondary structure found in protein - VERIFIED ANSWER
- is disrupted by proline, amine H and carbonyl O
,What can you conclude about the thermodynamic spontaneity of a
reaction that has a negative value for delta H and positive for delta S -
VERIFIED ANSWER - The reaction is spontaneous
The kinetics of most enzymatic reactions can be described in terms of Km
and Vmax. Which best describes Km? - VERIFIED ANSWER - the substrate
concentration required to give half maximal velocity
Which compound would penetrate a neutral membrane most rapidly by
free diffusion? - VERIFIED ANSWER - ethanol
The analysis of proteins by SDS-PAGE: - VERIFIED ANSWER - yields the
subunit molecular weights for an oligomeric protein
Gel filtration chromatography separates on the basis of - VERIFIED
ANSWER - size and shape
The conversion of a zymogen to an active proteolytic enzyme usually
occurs as a result of - VERIFIED ANSWER - Proteolytic cleavage of the
zymogen
, The carnitine derivatives of long chain fatty acids are used - VERIFIED
ANSWER - To transport fatty acids across the mitochondrial membrane
Which of these lipid classes contains glycosidic linkages - VERIFIED
ANSWER - ganglioside
G-proteins become active when they bind - VERIFIED ANSWER - GTP only
Hydroxyproline is: - VERIFIED ANSWER - a non-standard amino acid formed
by the post translational modification of a protein
A technique that is not widely used to amplify DNA sequences is: -
VERIFIED ANSWER - polymerase chain reaction
If the carboxyl carbon of palmitic acid (16:0) is the number 1 carbon,
which carbon of the original palmitate has been oxidized after the first
cycle of Beta-oxidation - VERIFIED ANSWER - C3
After the respiratory inhibitor cyanide is added to a suspension of
actively respiring, tightly coupled mitochondria... - VERIFIED ANSWER - The
rate of oxygen consumption will decrease
Comprehensive Biochemistry Study Guide 2026/ 2027
with solution (GRADED A+)
In setting up an assay for measuring the total amount of an enzyme
present in an unknown sample, the substrate concentration should
preferably be: - VERIFIED ANSWER - 10 fold higher than Km
Dialysis would be most useful for the separation of: - VERIFIED ANSWER - A
ammonium sulfate from an enzyme
An inhibitor that increases Km and does not alter the V max value is -
VERIFIED ANSWER - A competitive inhibitor
Which amino acid side chain would not be expected would not be expected
to participate in covalent enzyme catalysis - VERIFIED ANSWER - Leu
Which statement is correct concerning the T(deoxy) and R(oxy) states of
tetrameric hemoglobin - VERIFIED ANSWER - In the T to R transition, the
central cavity of the tetramer decreases in size, preventing the binding of
the allosteric modulator 2,3,-biophosphylglycerate
,When the energy needs of the cell are satisfied, acetyl-CoA accumulates
and serves as a positive allosteric effector for - VERIFIED ANSWER -
pyruvate carboxylase
To determine the Km value for an enzyme reaction, which parameter is
normally varied, while keeping the others constant - VERIFIED ANSWER -
substrate concentration
In a yeast cell growing aerobically on glucose, which would provide most
of the energy for growth? - VERIFIED ANSWER - glycolysis to TCA cycle
and oxidative phosphorylation
Cleavage of disulfide bonds in globular proteins may be brought about by -
VERIFIED ANSWER - Reaction with excess B-mercaptoethanol at pH 8 in 6
M urea
The alpha helical secondary structure found in protein - VERIFIED ANSWER
- is disrupted by proline, amine H and carbonyl O
,What can you conclude about the thermodynamic spontaneity of a
reaction that has a negative value for delta H and positive for delta S -
VERIFIED ANSWER - The reaction is spontaneous
The kinetics of most enzymatic reactions can be described in terms of Km
and Vmax. Which best describes Km? - VERIFIED ANSWER - the substrate
concentration required to give half maximal velocity
Which compound would penetrate a neutral membrane most rapidly by
free diffusion? - VERIFIED ANSWER - ethanol
The analysis of proteins by SDS-PAGE: - VERIFIED ANSWER - yields the
subunit molecular weights for an oligomeric protein
Gel filtration chromatography separates on the basis of - VERIFIED
ANSWER - size and shape
The conversion of a zymogen to an active proteolytic enzyme usually
occurs as a result of - VERIFIED ANSWER - Proteolytic cleavage of the
zymogen
, The carnitine derivatives of long chain fatty acids are used - VERIFIED
ANSWER - To transport fatty acids across the mitochondrial membrane
Which of these lipid classes contains glycosidic linkages - VERIFIED
ANSWER - ganglioside
G-proteins become active when they bind - VERIFIED ANSWER - GTP only
Hydroxyproline is: - VERIFIED ANSWER - a non-standard amino acid formed
by the post translational modification of a protein
A technique that is not widely used to amplify DNA sequences is: -
VERIFIED ANSWER - polymerase chain reaction
If the carboxyl carbon of palmitic acid (16:0) is the number 1 carbon,
which carbon of the original palmitate has been oxidized after the first
cycle of Beta-oxidation - VERIFIED ANSWER - C3
After the respiratory inhibitor cyanide is added to a suspension of
actively respiring, tightly coupled mitochondria... - VERIFIED ANSWER - The
rate of oxygen consumption will decrease
