ACS INSTRUMENTAL ANALYSIS
EXAM QUESTIONS WITH COMPLETE
SOLUTIONS
UV-Vis Source (Mono or Poly) - ANSWERSW-Halogen lamp; D2 or H2Lamp; Xenon
Lamp:(poly)
IR Source (Mono or Poly) - ANSWERSNernst Glower; Globular; Incandescent: (Poly)
Laser(Mono)
AA Source (mono or Poly) - ANSWERSHCl(mono)
AE Source (Mono or Poly) - ANSWERSPlasma Falme(poly)
Raman Source (mono or Poly) - ANSWERSHe/Ne Laser;Ar Laser;Yag Laser:(mono)
UV-Vis Sample Holder - ANSWERSQuartz, Glass, Plastic Cuvettes
IR Sample Holder - ANSWERSsalt plat; gas chamber
AA Sample Holder - ANSWERSGraphite Tube; Flame
AE Sample Holder - ANSWERSinductively coupled plasma (ICP)
Raman Sample Holder - ANSWERSCuvette
UV-Vis Wavelength Selector - ANSWERSGrating, Prism, Filter
IR Wavelength Selector - ANSWERSInterferometer; monochromator
AA Wavelength Selector - ANSWERSMonochrom. w/ grating
AE Wavelength Selector - ANSWERSMonochrom.
Raman Wavelength Selector - ANSWERSMonochrom. w/ grating; Infermom. w/ laser
UV-Vis Detector - ANSWERSPhotomultiplier Tube(PMT); Photodiode Array
IR Detector - ANSWERSThermal(dispersion); pyroelectric; photoconducting
AA Detector - ANSWERSPMT
, AE Detector - ANSWERSPMT; Photodiode Array
Raman Detector - ANSWERSPMT
HPLC Mobile Phase - ANSWERSRev Phase: Polar Solvent
Norm. Phase: Nonpolar Solvent
GC Mobile Phase - ANSWERSHe Gas
HPLC Column Length - ANSWERS10-30 cm
GC Column length - ANSWERS30-50 m
HPLC Modes of Operation - ANSWERS1. Isocratic
2. Gradient
GC Modes of Operation - ANSWERS1.Isothermal
2. Temp program
HPLC Injection System and Amount - ANSWERSLoop System (10uL)
GC Injection Syst. and Amount - ANSWERSSyringe (3-5uL)
HPLC Sationary Phase - ANSWERSNP=Polar
RP=Nonpolar
HPLC Detector - ANSWERSZ cell (UV)
GC Detector - ANSWERSFID (flame ionization detector)
HPLC Pump - ANSWERSReciprocating
Displacement
HPLC Chromotography - ANSWERS1. Partition
2. Adsorption
3. Ion Exchange
4. Size Exclusion
5. Affinity
what are the difference between calibration sensitivity and analytical sensitivity -
ANSWERScalibration sensitivity is the slope of a calibration curve at the concentration
of interest while analytical sensitivity is a response to the noise ratio
how to calculate calibration sensitivity - ANSWERSy=mx+b (m is the calibration
sensitivity)
graph concentration vs mean analytical signal
EXAM QUESTIONS WITH COMPLETE
SOLUTIONS
UV-Vis Source (Mono or Poly) - ANSWERSW-Halogen lamp; D2 or H2Lamp; Xenon
Lamp:(poly)
IR Source (Mono or Poly) - ANSWERSNernst Glower; Globular; Incandescent: (Poly)
Laser(Mono)
AA Source (mono or Poly) - ANSWERSHCl(mono)
AE Source (Mono or Poly) - ANSWERSPlasma Falme(poly)
Raman Source (mono or Poly) - ANSWERSHe/Ne Laser;Ar Laser;Yag Laser:(mono)
UV-Vis Sample Holder - ANSWERSQuartz, Glass, Plastic Cuvettes
IR Sample Holder - ANSWERSsalt plat; gas chamber
AA Sample Holder - ANSWERSGraphite Tube; Flame
AE Sample Holder - ANSWERSinductively coupled plasma (ICP)
Raman Sample Holder - ANSWERSCuvette
UV-Vis Wavelength Selector - ANSWERSGrating, Prism, Filter
IR Wavelength Selector - ANSWERSInterferometer; monochromator
AA Wavelength Selector - ANSWERSMonochrom. w/ grating
AE Wavelength Selector - ANSWERSMonochrom.
Raman Wavelength Selector - ANSWERSMonochrom. w/ grating; Infermom. w/ laser
UV-Vis Detector - ANSWERSPhotomultiplier Tube(PMT); Photodiode Array
IR Detector - ANSWERSThermal(dispersion); pyroelectric; photoconducting
AA Detector - ANSWERSPMT
, AE Detector - ANSWERSPMT; Photodiode Array
Raman Detector - ANSWERSPMT
HPLC Mobile Phase - ANSWERSRev Phase: Polar Solvent
Norm. Phase: Nonpolar Solvent
GC Mobile Phase - ANSWERSHe Gas
HPLC Column Length - ANSWERS10-30 cm
GC Column length - ANSWERS30-50 m
HPLC Modes of Operation - ANSWERS1. Isocratic
2. Gradient
GC Modes of Operation - ANSWERS1.Isothermal
2. Temp program
HPLC Injection System and Amount - ANSWERSLoop System (10uL)
GC Injection Syst. and Amount - ANSWERSSyringe (3-5uL)
HPLC Sationary Phase - ANSWERSNP=Polar
RP=Nonpolar
HPLC Detector - ANSWERSZ cell (UV)
GC Detector - ANSWERSFID (flame ionization detector)
HPLC Pump - ANSWERSReciprocating
Displacement
HPLC Chromotography - ANSWERS1. Partition
2. Adsorption
3. Ion Exchange
4. Size Exclusion
5. Affinity
what are the difference between calibration sensitivity and analytical sensitivity -
ANSWERScalibration sensitivity is the slope of a calibration curve at the concentration
of interest while analytical sensitivity is a response to the noise ratio
how to calculate calibration sensitivity - ANSWERSy=mx+b (m is the calibration
sensitivity)
graph concentration vs mean analytical signal
