MB (ASCP) EXAM 2025-2026 QUESTIONS
AND CORRECT ANSWERS GRADED A+
What kind of DNA is most likely to be transcriptionally active?
Euchromatin
What is the most common type of eukaryotic DNA?
Satellite DNA
What specimen has the most DNA according to molecular weight?
White Blood Cells
What amino acids compose histones?
Lysine and Arginine
Amino acid that all proteins start with:
Methionine
,What protects the DNA strand during replication?
Single-stranded binding protein
Leading Strand
3' to 5' template
Lagging Strand
5' to 3' template
What DNA strand is being copied in transcription?
Antisense Strand
First step after newly formed mRNA is made?
Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme?
Telomerase
What polymerase has repair activity?
,Beta/Epsilon
What polymerase acts on leading strand?
Delta
What polymerase acts on lagging strand?
Alpha
Which polymerase is used for mitochondrial replication and repair?
Gamma
What polymerase binds the 5' end to 3' end of dsDNA?
Topoisomerase
Which RNA polymerase is mainly for transcription?
RNA Pol II
Which polymerase is involved with mRNA synthesis?
RNA Pol II
, What inactivates RNAses?
Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites?
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity?
Restriction Enzyme Type II
What is necessary to make cDNA?
Reverse transcriptase
Number of SNPs in a human genome?
11 billion (10^7)
How many genes (base pairs) are in a human haploid genome?
3 billion base pairs
AND CORRECT ANSWERS GRADED A+
What kind of DNA is most likely to be transcriptionally active?
Euchromatin
What is the most common type of eukaryotic DNA?
Satellite DNA
What specimen has the most DNA according to molecular weight?
White Blood Cells
What amino acids compose histones?
Lysine and Arginine
Amino acid that all proteins start with:
Methionine
,What protects the DNA strand during replication?
Single-stranded binding protein
Leading Strand
3' to 5' template
Lagging Strand
5' to 3' template
What DNA strand is being copied in transcription?
Antisense Strand
First step after newly formed mRNA is made?
Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme?
Telomerase
What polymerase has repair activity?
,Beta/Epsilon
What polymerase acts on leading strand?
Delta
What polymerase acts on lagging strand?
Alpha
Which polymerase is used for mitochondrial replication and repair?
Gamma
What polymerase binds the 5' end to 3' end of dsDNA?
Topoisomerase
Which RNA polymerase is mainly for transcription?
RNA Pol II
Which polymerase is involved with mRNA synthesis?
RNA Pol II
, What inactivates RNAses?
Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites?
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity?
Restriction Enzyme Type II
What is necessary to make cDNA?
Reverse transcriptase
Number of SNPs in a human genome?
11 billion (10^7)
How many genes (base pairs) are in a human haploid genome?
3 billion base pairs
