ASCP MOLECULAR BIOLOGY CERTIFICATION
EXAM 2026-2027 QUESTIONS AD CORRECT
ANSWERS GRADED A+
Methylation of cytosine bases 5' to the gene will increase or decrease expression?
decrease
Histone acetylation close to the gene will increase or decrease expression?
increase
siRNAs complementary to the gene transcript will increase or decrease expression?
decrease
Calculate the DNA concentration from the following:
260=0.172 (D.F. 1:100)
860 ug/mL = .172 abs 50 ug/mL 100 DF
,[DNA] = 1535 ug/mL. You have 0.5 mL. What is the total yield.
767.5 ug = 1535 ug/mL * 0.5mL
[DNA] = 767.5 ug/mL. You have 0.5 mL What is the total yield.
383.75 ug = 767.5 ug/mL * 0.5 mL
[DNA] = 860 ug/mL. You have 0.5 mL. What is the total yield.
430 ug = 860 ug/mL * 0.5mL
Calculate the RNA concentration from the following:
260=0.307 (DF 1:100)
1228 ug/mL = 0.307 abs 40 ug/mL 100 DF
An RNA preparation has the following readings:
260=0.208
,280=0.096
Is this RNA suitable for use?
Yes, 2.17 is suitable for RNA analysis
A260/A280 = 0.208/0.096
How does PFGE separate larger fragments more efficiently than standard
electrophoresis?
Repeated reorientation forces larger fragments through the gel matrix more
efficiently
If fragments are dissolved in 50% formamide will the stringency be higher or
lower?
Higher
If fragments are dissolved in a high concentration of NaCl will the stringency be
higher or lower?
Lower
, Does heating a solution from 65C to 75C during hybridization raise or lower
stringency?
raises
What would the autoradiogram show if the stringency was to high?
no bands
Calculate the Tm of the following primers:
Forward: GGAGCTTTGTTTCAACCAAG
Reverse: ATTAAATGCGGAATTGCCCA
Forward: Tm=(4C x 9GC)+(2C x 11AT) = 58C
Reverse Tm=(4C x 8GC)+(2C x 12AT) = 56C
Is 47;XXY a normal karyotype?
No, this is XXY syndrome
What is the genetic abnormality of:
47,XY,+18
EXAM 2026-2027 QUESTIONS AD CORRECT
ANSWERS GRADED A+
Methylation of cytosine bases 5' to the gene will increase or decrease expression?
decrease
Histone acetylation close to the gene will increase or decrease expression?
increase
siRNAs complementary to the gene transcript will increase or decrease expression?
decrease
Calculate the DNA concentration from the following:
260=0.172 (D.F. 1:100)
860 ug/mL = .172 abs 50 ug/mL 100 DF
,[DNA] = 1535 ug/mL. You have 0.5 mL. What is the total yield.
767.5 ug = 1535 ug/mL * 0.5mL
[DNA] = 767.5 ug/mL. You have 0.5 mL What is the total yield.
383.75 ug = 767.5 ug/mL * 0.5 mL
[DNA] = 860 ug/mL. You have 0.5 mL. What is the total yield.
430 ug = 860 ug/mL * 0.5mL
Calculate the RNA concentration from the following:
260=0.307 (DF 1:100)
1228 ug/mL = 0.307 abs 40 ug/mL 100 DF
An RNA preparation has the following readings:
260=0.208
,280=0.096
Is this RNA suitable for use?
Yes, 2.17 is suitable for RNA analysis
A260/A280 = 0.208/0.096
How does PFGE separate larger fragments more efficiently than standard
electrophoresis?
Repeated reorientation forces larger fragments through the gel matrix more
efficiently
If fragments are dissolved in 50% formamide will the stringency be higher or
lower?
Higher
If fragments are dissolved in a high concentration of NaCl will the stringency be
higher or lower?
Lower
, Does heating a solution from 65C to 75C during hybridization raise or lower
stringency?
raises
What would the autoradiogram show if the stringency was to high?
no bands
Calculate the Tm of the following primers:
Forward: GGAGCTTTGTTTCAACCAAG
Reverse: ATTAAATGCGGAATTGCCCA
Forward: Tm=(4C x 9GC)+(2C x 11AT) = 58C
Reverse Tm=(4C x 8GC)+(2C x 12AT) = 56C
Is 47;XXY a normal karyotype?
No, this is XXY syndrome
What is the genetic abnormality of:
47,XY,+18
