AST - Aspartate Aminotransferase Preparedness Baseline Exam Knowledge Questions with Expertly written solution 2025/2026

AST - Aspartate Aminotransferase Preparedness Baseline
Exam Knowledge Questions with Expertly written solution
2025/2026


Reaction of AST - correct answer transfer of an amino group between
aspartate and alpha keto acid


Physiological reaction of AST - correct answer aspartic acid + alpha
ketoglutarate --> oxaloacetate + glutamate


Coenzyme for AST - correct answer pyridoxal phosphate


Function of AST - correct answer Important in intermediate metabolism
Synthesis and degradation of amino acid


Tissue sources of AST - correct answer Cardiac tissue, liver, skeletal
muscle


Clinical importance of AST - correct answer -Was used to evaluate
myocardial infarction
-Evaluates skeletal muscle disease (muscular dystrophies)
-Useful in detecting liver damage and evaluate hepatocellular disease


Increased AST - correct answer AMI
Skeletal muscle disorders
Hemolytic anemia
Pulmonary embolism and congestive heart failure
Acute hepatic disorders

, Principle use of AST - correct answer LFT (liver function test)
AST is more sensitive to hepatocellular damage than ALT which is specific


Decreased AST - correct answer -Uremia
-Vitamin B deficiency
-Administration of some drugs


2 forms of AST isoenzymes - correct answer 1- Found in cell cytoplasm
2- Found in mitochondria


Assay used for AST - correct answer Karmen Method


Samples used for AST - correct answer Serum and plasma (non
hemolyzed)


ALT class - correct answer Transferase


ALT group - correct answer transaminase


ALT reaction - correct answer Catalyzes the reversible transfer of amino
acid


Physiological reaction of ALT - correct answer Alanine + alpha
ketoglutarate --> pyruvate + glutamate


ALT coenzyme - correct answer Pyridoxal phosphate (vitamin B6)