Effect of Alcohol on Brine Shrimp Activity
Sara Entrekin & Deborah Akiwowo
Biological Principles 1 Laboratory, Kennesaw State University
Methods
1. Label 4 test tubes and 4 petri dishes with 100%, 50%, 25%, 0%. 7. Lastly take 2.5mL of sea water and put into “0%” test tube
Introduction 2. Use a pipette to fill 2.5mL of alcohol into the “100%” test tube.
3. Fill 2.5mL of alcohol into the “50%” test test with the same pipette.
because it is pure seawater, no alcohol
8. Pour each of the test tube solutions into the matching Conclusions
Artemia, more commonly known as brine 4. Using a new pipette put 2.5mL of sea water into the same “50%” labeled petri dishes
test tube to make the solution 50% diluted alcohol. 9. Place the “100%” petri dish under the microscope and take As the results have suggested, our hypothesis has
shrimp, are habitants of high salinity water
5. Using a new pipette, take 2.5mL of the 50% solution and put into 2.5mL of the brine shrimp into a new pipette, watch shrimp been proven to show that different alcohol
sources, such as salt lakes, that are often the “25%” test tube activity after pouring the brine shrimp into the petri dish
studied to compare the organisms reaction concentrations did in fact affect the brine shrimp
6. Put a new 2.5mL of sea water into the “25%” test tube to make 25% 10. Repeat previous step with each of the labeled petri dishes
under different conditions. Brine shrimp are diluted alcohol solution and record the activity in each solution differently. We can also support our prediction based
one of many small, simple organisms that on the data we collected that increased alcohol
biologists experiment with in order to learn Add brine concentrations lead to decreased activity and survival
how organisms behave in various Create the 4 Transfer Record results of rates. Even though we used brine shrimp to study the
environments. We did a study on brine shrimp various alcohol shrimp to each brine shrimp activity
solutions to reaction to alcohol, this experiment can also correlate
activity and survival rate in the presence of concentration solution under and survival rates in to humans. Drinking alcohol negatively affects the
alcohol. The independent variables were the
petri dishes the microscope
solutions each solution body and brain, resulting in decreased activity and
different concentrations of alcohol in the salt even death in some cases. Our independent variable,
water. The brine shrimp’s reaction to solution
different alcohol concentrations, directly impacts the
that affected the activity level and survival rate
were the dependent variables. We
Results dependent variables, activity levels and survival rates.
hypothesized that the different concentrations The more alcohol a person drinks, the less they will be
of alcohol would affect the brine shrimp Figure 2: alcohol concentration Table 1: alcohol concentration able to control their body and some instances lead to
differently. During the week 1 brine shrimp alcohol poisoning and bad decisions that unfortunately
salinity experiment, we discovered that the in salt water vs shrimp activity vs shrimp activity & survival rate lead to death. According to the CDC, there are over
shrimp were more active when the there was a 140,000 annual deaths in the United States caused by
higher concentration of salt in the water. shrimp ac vity Alcohol Shrimp activity (0-5 with Amount of dead shrimp
Place graph here
uncontrolled alcohol consumption. This problem can
Based on those results, we predicted that if 100 concentration 0 = no activity, 5 = lots (0-5, 0 = no deaths, 5 =
be simply represented by our experiment on brine
more alcohol was present, the less active the activity) all/almost all dead
75 shrimp through the relation between alcohol levels and
brine shrimp will be, therefore lower survival
rates, due to the decreased salinity in higher 100% 1 5 results following.
alcohol concentrations. 50 50% 3 2 Limitations and Assumptions
Figure 1: brine shrimp 25 25% 4 1 Many things can go wrong in this experiment so
0 it is very important to follow through the steps
0% 5 0 accordingly, especially when creating the
4M 2M 1M 0.5M 0M solutions. As stated previously, the data collected
Figure 2: numbers on the left represents the was purely qualitative. A better approach would
Table 1: displays the estimated ratings of the shrimp’s
estimated percentage of activity in various alcohol be to actually count the deaths to provided a
activity and survival in the various alcohol concentrations
concentrations, numbers across the bottom. more unbiased result.
Results: both the bar graph (figure 2) and the chart (table 1) represent qualitative data. This References
means that the data collected was based on our personal observations and comparisons of 1. Department of Cellular and Molecular Biology, Kennesaw State university.
(2019). Brine shrimp (Artemia) Experiment.
the brine shrimps reactions in each of the alcohol solutions. According to the graph, at 1M of
Figure 1: picture of a brine shrimp the NaCl solution, the shrimp activity is the highest, 100%. High salinity and lower salinity from 2. Urry, L. A., Cain, M. L. 1., Wasserman, S. A., Minorsky, P. V., Reece, J. B.,
& Campbell, N. A. (2017). Essential biology. Eleventh edition. New York, NY:
https://cdn.britannica.com/ the 1M, shrimp activity decreased. In the table, at 100% alcohol concentration, shrimp activity Pearson Education, Inc.
63/221363-050-531CF632/shrimp.jpg is the lowest, 1 rating, and the highest amount of dead shrimp, 5 rating. As the alcohol 3.“Deaths from Excessive Alcohol Use in the United States.” Centers for
concentration decreases, both shrimp activity and survival rates increase. Disease Control and Prevention, Centers for Disease Control and
Prevention, 6 July 2022, https://www.cdc.gov/alcohol/features/excessive-
alcohol-deaths.html.
ti
Sara Entrekin & Deborah Akiwowo
Biological Principles 1 Laboratory, Kennesaw State University
Methods
1. Label 4 test tubes and 4 petri dishes with 100%, 50%, 25%, 0%. 7. Lastly take 2.5mL of sea water and put into “0%” test tube
Introduction 2. Use a pipette to fill 2.5mL of alcohol into the “100%” test tube.
3. Fill 2.5mL of alcohol into the “50%” test test with the same pipette.
because it is pure seawater, no alcohol
8. Pour each of the test tube solutions into the matching Conclusions
Artemia, more commonly known as brine 4. Using a new pipette put 2.5mL of sea water into the same “50%” labeled petri dishes
test tube to make the solution 50% diluted alcohol. 9. Place the “100%” petri dish under the microscope and take As the results have suggested, our hypothesis has
shrimp, are habitants of high salinity water
5. Using a new pipette, take 2.5mL of the 50% solution and put into 2.5mL of the brine shrimp into a new pipette, watch shrimp been proven to show that different alcohol
sources, such as salt lakes, that are often the “25%” test tube activity after pouring the brine shrimp into the petri dish
studied to compare the organisms reaction concentrations did in fact affect the brine shrimp
6. Put a new 2.5mL of sea water into the “25%” test tube to make 25% 10. Repeat previous step with each of the labeled petri dishes
under different conditions. Brine shrimp are diluted alcohol solution and record the activity in each solution differently. We can also support our prediction based
one of many small, simple organisms that on the data we collected that increased alcohol
biologists experiment with in order to learn Add brine concentrations lead to decreased activity and survival
how organisms behave in various Create the 4 Transfer Record results of rates. Even though we used brine shrimp to study the
environments. We did a study on brine shrimp various alcohol shrimp to each brine shrimp activity
solutions to reaction to alcohol, this experiment can also correlate
activity and survival rate in the presence of concentration solution under and survival rates in to humans. Drinking alcohol negatively affects the
alcohol. The independent variables were the
petri dishes the microscope
solutions each solution body and brain, resulting in decreased activity and
different concentrations of alcohol in the salt even death in some cases. Our independent variable,
water. The brine shrimp’s reaction to solution
different alcohol concentrations, directly impacts the
that affected the activity level and survival rate
were the dependent variables. We
Results dependent variables, activity levels and survival rates.
hypothesized that the different concentrations The more alcohol a person drinks, the less they will be
of alcohol would affect the brine shrimp Figure 2: alcohol concentration Table 1: alcohol concentration able to control their body and some instances lead to
differently. During the week 1 brine shrimp alcohol poisoning and bad decisions that unfortunately
salinity experiment, we discovered that the in salt water vs shrimp activity vs shrimp activity & survival rate lead to death. According to the CDC, there are over
shrimp were more active when the there was a 140,000 annual deaths in the United States caused by
higher concentration of salt in the water. shrimp ac vity Alcohol Shrimp activity (0-5 with Amount of dead shrimp
Place graph here
uncontrolled alcohol consumption. This problem can
Based on those results, we predicted that if 100 concentration 0 = no activity, 5 = lots (0-5, 0 = no deaths, 5 =
be simply represented by our experiment on brine
more alcohol was present, the less active the activity) all/almost all dead
75 shrimp through the relation between alcohol levels and
brine shrimp will be, therefore lower survival
rates, due to the decreased salinity in higher 100% 1 5 results following.
alcohol concentrations. 50 50% 3 2 Limitations and Assumptions
Figure 1: brine shrimp 25 25% 4 1 Many things can go wrong in this experiment so
0 it is very important to follow through the steps
0% 5 0 accordingly, especially when creating the
4M 2M 1M 0.5M 0M solutions. As stated previously, the data collected
Figure 2: numbers on the left represents the was purely qualitative. A better approach would
Table 1: displays the estimated ratings of the shrimp’s
estimated percentage of activity in various alcohol be to actually count the deaths to provided a
activity and survival in the various alcohol concentrations
concentrations, numbers across the bottom. more unbiased result.
Results: both the bar graph (figure 2) and the chart (table 1) represent qualitative data. This References
means that the data collected was based on our personal observations and comparisons of 1. Department of Cellular and Molecular Biology, Kennesaw State university.
(2019). Brine shrimp (Artemia) Experiment.
the brine shrimps reactions in each of the alcohol solutions. According to the graph, at 1M of
Figure 1: picture of a brine shrimp the NaCl solution, the shrimp activity is the highest, 100%. High salinity and lower salinity from 2. Urry, L. A., Cain, M. L. 1., Wasserman, S. A., Minorsky, P. V., Reece, J. B.,
& Campbell, N. A. (2017). Essential biology. Eleventh edition. New York, NY:
https://cdn.britannica.com/ the 1M, shrimp activity decreased. In the table, at 100% alcohol concentration, shrimp activity Pearson Education, Inc.
63/221363-050-531CF632/shrimp.jpg is the lowest, 1 rating, and the highest amount of dead shrimp, 5 rating. As the alcohol 3.“Deaths from Excessive Alcohol Use in the United States.” Centers for
concentration decreases, both shrimp activity and survival rates increase. Disease Control and Prevention, Centers for Disease Control and
Prevention, 6 July 2022, https://www.cdc.gov/alcohol/features/excessive-
alcohol-deaths.html.
ti
