ACS INSTRUMENTAL ANALYSIS
QUESTIONS & ANSWERS
UV-Vis Source (Mono or Poly) - ANSWERW-Halogen lamp; D2 or H2Lamp; Xenon
Lamp:(poly)
IR Source (Mono or Poly) - ANSWERNernst Glower; Globular; Incandescent: (Poly)
Laser(Mono)
AA Source (mono or Poly) - ANSWERHCl(mono)
AE Source (Mono or Poly) - ANSWERPlasma Falme(poly)
Raman Source (mono or Poly) - ANSWERHe/Ne Laser;Ar Laser;Yag Laser:(mono)
UV-Vis Sample Holder - ANSWERQuartz, Glass, Plastic Cuvettes
IR Sample Holder - ANSWERsalt plat; gas chamber
AA Sample Holder - ANSWERGraphite Tube; Flame
AE Sample Holder - ANSWERinductively coupled plasma (ICP)
Raman Sample Holder - ANSWERCuvette
UV-Vis Wavelength Selector - ANSWERGrating, Prism, Filter
IR Wavelength Selector - ANSWERInterferometer; monochromator
AA Wavelength Selector - ANSWERMonochrom. w/ grating
AE Wavelength Selector - ANSWERMonochrom.
Raman Wavelength Selector - ANSWERMonochrom. w/ grating; Infermom. w/ laser
UV-Vis Detector - ANSWERPhotomultiplier Tube(PMT); Photodiode Array
IR Detector - ANSWERThermal(dispersion); pyroelectric; photoconducting
AA Detector - ANSWERPMT
AE Detector - ANSWERPMT; Photodiode Array
Raman Detector - ANSWERPMT
, HPLC Mobile Phase - ANSWERRev Phase: Polar Solvent
Norm. Phase: Nonpolar Solvent
GC Mobile Phase - ANSWERHe Gas
HPLC Column Length - ANSWER10-30 cm
GC Column length - ANSWER30-50 m
HPLC Modes of Operation - ANSWER1. Isocratic
2. Gradient
GC Modes of Operation - ANSWER1.Isothermal
2. Temp program
HPLC Injection System and Amount - ANSWERLoop System (10uL)
GC Injection Syst. and Amount - ANSWERSyringe (3-5uL)
HPLC Sationary Phase - ANSWERNP=Polar
RP=Nonpolar
HPLC Detector - ANSWERZ cell (UV)
GC Detector - ANSWERFID (flame ionization detector)
HPLC Pump - ANSWERReciprocating
Displacement
HPLC Chromotography - ANSWER1. Partition
2. Adsorption
3. Ion Exchange
4. Size Exclusion
5. Affinity
what are the difference between calibration sensitivity and analytical sensitivity -
ANSWERcalibration sensitivity is the slope of a calibration curve at the concentration of
interest while analytical sensitivity is a response to the noise ratio
how to calculate calibration sensitivity - ANSWERy=mx+b (m is the calibration
sensitivity)
graph concentration vs mean analytical signal
how to find the analytical sensitivity - ANSWERAs (concentation) = calibration
sensitivity/SD [ ]
QUESTIONS & ANSWERS
UV-Vis Source (Mono or Poly) - ANSWERW-Halogen lamp; D2 or H2Lamp; Xenon
Lamp:(poly)
IR Source (Mono or Poly) - ANSWERNernst Glower; Globular; Incandescent: (Poly)
Laser(Mono)
AA Source (mono or Poly) - ANSWERHCl(mono)
AE Source (Mono or Poly) - ANSWERPlasma Falme(poly)
Raman Source (mono or Poly) - ANSWERHe/Ne Laser;Ar Laser;Yag Laser:(mono)
UV-Vis Sample Holder - ANSWERQuartz, Glass, Plastic Cuvettes
IR Sample Holder - ANSWERsalt plat; gas chamber
AA Sample Holder - ANSWERGraphite Tube; Flame
AE Sample Holder - ANSWERinductively coupled plasma (ICP)
Raman Sample Holder - ANSWERCuvette
UV-Vis Wavelength Selector - ANSWERGrating, Prism, Filter
IR Wavelength Selector - ANSWERInterferometer; monochromator
AA Wavelength Selector - ANSWERMonochrom. w/ grating
AE Wavelength Selector - ANSWERMonochrom.
Raman Wavelength Selector - ANSWERMonochrom. w/ grating; Infermom. w/ laser
UV-Vis Detector - ANSWERPhotomultiplier Tube(PMT); Photodiode Array
IR Detector - ANSWERThermal(dispersion); pyroelectric; photoconducting
AA Detector - ANSWERPMT
AE Detector - ANSWERPMT; Photodiode Array
Raman Detector - ANSWERPMT
, HPLC Mobile Phase - ANSWERRev Phase: Polar Solvent
Norm. Phase: Nonpolar Solvent
GC Mobile Phase - ANSWERHe Gas
HPLC Column Length - ANSWER10-30 cm
GC Column length - ANSWER30-50 m
HPLC Modes of Operation - ANSWER1. Isocratic
2. Gradient
GC Modes of Operation - ANSWER1.Isothermal
2. Temp program
HPLC Injection System and Amount - ANSWERLoop System (10uL)
GC Injection Syst. and Amount - ANSWERSyringe (3-5uL)
HPLC Sationary Phase - ANSWERNP=Polar
RP=Nonpolar
HPLC Detector - ANSWERZ cell (UV)
GC Detector - ANSWERFID (flame ionization detector)
HPLC Pump - ANSWERReciprocating
Displacement
HPLC Chromotography - ANSWER1. Partition
2. Adsorption
3. Ion Exchange
4. Size Exclusion
5. Affinity
what are the difference between calibration sensitivity and analytical sensitivity -
ANSWERcalibration sensitivity is the slope of a calibration curve at the concentration of
interest while analytical sensitivity is a response to the noise ratio
how to calculate calibration sensitivity - ANSWERy=mx+b (m is the calibration
sensitivity)
graph concentration vs mean analytical signal
how to find the analytical sensitivity - ANSWERAs (concentation) = calibration
sensitivity/SD [ ]
