BIOCHEM 210 MODULE 7 EXAM
2026/2027 | Metabolism: Glycolysis,
Citric Acid Cycle & Oxidative
Phosphorylation | Latest Q&A Verified |
Portage Learning | Pass Guaranteed - A+
Graded
Glycolysis (Questions 1-35)
1. Glycolysis occurs in which cellular compartment?
A) Mitochondrial matrix
B) Cytoplasm
C) Inner mitochondrial membrane
D) Nucleus
Rationale: Glycolysis takes place in the cytosol of all cells.
2. The net ATP yield from one molecule of glucose via glycolysis (substrate-level
phosphorylation only) is:
A) 4 ATP
B) 2 ATP
C) 1 ATP
D) 0 ATP
,Rationale: Two ATP are used in the energy investment phase; four ATP are produced
in the energy payoff phase; net = 2 ATP.
3. Which enzyme catalyzes the committed step of glycolysis?
A) Hexokinase
B) Phosphofructokinase-1 (PFK-1)
C) Aldolase
D) PFK-1
Rationale: PFK-1 is the key regulated step; it commits glucose to glycolysis.
4. In anaerobic conditions, pyruvate is converted to lactate by:
A) Pyruvate decarboxylase
B) Lactate dehydrogenase
C) Pyruvate kinase
D) Alcohol dehydrogenase
Rationale: Lactate dehydrogenase reduces pyruvate to lactate, regenerating NAD⁺.
5. The end product of glycolysis under aerobic conditions is:
A) Lactate
B) Pyruvate
C) Acetyl-CoA
D) Glucose-6-phosphate
Rationale: Pyruvate is the final product; it enters the mitochondria for further
oxidation.
6. Which step in glycolysis produces NADH?
A) Hexokinase
B) Phosphofructokinase
C) Pyruvate kinase
D) Glyceraldehyde-3-phosphate dehydrogenase
Rationale: GAPDH oxidizes G3P and reduces NAD⁺ to NADH.
, 7. The enzyme that adds a phosphate group to glucose using ATP is:
A) Phosphoglucose isomerase
B) Hexokinase
C) Glucokinase (liver)
D) Both B and C
Rationale: Hexokinase (ubiquitous) and glucokinase (liver) catalyze the first
phosphorylation.
8. Which intermediate is cleaved into two triose phosphates?
A) Fructose-1,6-bisphosphate
B) Glucose-6-phosphate
C) Fructose-1,6-bisphosphate
D) Dihydroxyacetone phosphate
Rationale: Aldolase splits fructose-1,6-bisphosphate into G3P and DHAP.
9. The conversion of 3-phosphoglycerate to 2-phosphoglycerate is catalyzed by:
A) Enolase
B) Phosphoglycerate mutase
C) Glyceraldehyde-3-phosphate dehydrogenase
D) Triose phosphate isomerase
Rationale: Phosphoglycerate mutase shifts the phosphate group from C-3 to C-2.
10. Substrate-level phosphorylation in glycolysis occurs at which enzyme(s)?
A) Hexokinase and PFK-1
B) Phosphoglycerate kinase and pyruvate kinase
C) Aldolase and enolase
D) GAPDH and triose phosphate isomerase
Rationale: PGK produces ATP from 1,3-BPG; pyruvate kinase produces ATP from
PEP.
11. Which of the following allosterically activates PFK-1?
A) ATP
2026/2027 | Metabolism: Glycolysis,
Citric Acid Cycle & Oxidative
Phosphorylation | Latest Q&A Verified |
Portage Learning | Pass Guaranteed - A+
Graded
Glycolysis (Questions 1-35)
1. Glycolysis occurs in which cellular compartment?
A) Mitochondrial matrix
B) Cytoplasm
C) Inner mitochondrial membrane
D) Nucleus
Rationale: Glycolysis takes place in the cytosol of all cells.
2. The net ATP yield from one molecule of glucose via glycolysis (substrate-level
phosphorylation only) is:
A) 4 ATP
B) 2 ATP
C) 1 ATP
D) 0 ATP
,Rationale: Two ATP are used in the energy investment phase; four ATP are produced
in the energy payoff phase; net = 2 ATP.
3. Which enzyme catalyzes the committed step of glycolysis?
A) Hexokinase
B) Phosphofructokinase-1 (PFK-1)
C) Aldolase
D) PFK-1
Rationale: PFK-1 is the key regulated step; it commits glucose to glycolysis.
4. In anaerobic conditions, pyruvate is converted to lactate by:
A) Pyruvate decarboxylase
B) Lactate dehydrogenase
C) Pyruvate kinase
D) Alcohol dehydrogenase
Rationale: Lactate dehydrogenase reduces pyruvate to lactate, regenerating NAD⁺.
5. The end product of glycolysis under aerobic conditions is:
A) Lactate
B) Pyruvate
C) Acetyl-CoA
D) Glucose-6-phosphate
Rationale: Pyruvate is the final product; it enters the mitochondria for further
oxidation.
6. Which step in glycolysis produces NADH?
A) Hexokinase
B) Phosphofructokinase
C) Pyruvate kinase
D) Glyceraldehyde-3-phosphate dehydrogenase
Rationale: GAPDH oxidizes G3P and reduces NAD⁺ to NADH.
, 7. The enzyme that adds a phosphate group to glucose using ATP is:
A) Phosphoglucose isomerase
B) Hexokinase
C) Glucokinase (liver)
D) Both B and C
Rationale: Hexokinase (ubiquitous) and glucokinase (liver) catalyze the first
phosphorylation.
8. Which intermediate is cleaved into two triose phosphates?
A) Fructose-1,6-bisphosphate
B) Glucose-6-phosphate
C) Fructose-1,6-bisphosphate
D) Dihydroxyacetone phosphate
Rationale: Aldolase splits fructose-1,6-bisphosphate into G3P and DHAP.
9. The conversion of 3-phosphoglycerate to 2-phosphoglycerate is catalyzed by:
A) Enolase
B) Phosphoglycerate mutase
C) Glyceraldehyde-3-phosphate dehydrogenase
D) Triose phosphate isomerase
Rationale: Phosphoglycerate mutase shifts the phosphate group from C-3 to C-2.
10. Substrate-level phosphorylation in glycolysis occurs at which enzyme(s)?
A) Hexokinase and PFK-1
B) Phosphoglycerate kinase and pyruvate kinase
C) Aldolase and enolase
D) GAPDH and triose phosphate isomerase
Rationale: PGK produces ATP from 1,3-BPG; pyruvate kinase produces ATP from
PEP.
11. Which of the following allosterically activates PFK-1?
A) ATP
