Rats And Mice Ultimate General Exam Study
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determining gender in mice and rats
Observation of the anogenital distance
six basic areas animal rights activists focus on
a. Animal exploitation—advocates of bull fighting and cock fighting b. Animal use—
laboratories, hunting, fishing, purebred breeders, etc. c. Animal control—USDA, AVMA
d. Animal welfare—Animal Shelters e. Animal rights—No-kill shelters f. Animal
liberation—PETA
AAALAC
Association for Assessment and Accreditation of Laboratory Animal Care. Accreditation
signifies
a commitment of excellence.
Animal Welfare Act (AWA) covers
The sale, handling, transport, and use of animals used in education, research, or
exhibition
IACUC Committee members include
a. One veterinarian with training and experience in laboratory animal
b. One person that has no affiliation with the institution
Primary reference on animal care and use is called____________, created
by__________________.
a. The Guide for the Care and Use of Laboratory Animals.
b. Institute for Laboratory Animal Research (ILAR)
the three "R"s in research
a. Reduction—use the fewest number of animals possible to achieve research goals
b. Refinement—least amount of pain, stress, anxiety, and disturbance of normal life for
the animal
,c. Replacement—use the lower forms of life. Replace living models with nonliving
models, if possible
laboratory animal
Any vertebrate animal produced for or used in research, testing, or teaching
Institutional Officer (IO)
Representative of senior administration; bears ultimate responsibility for the Program
advances made as a result of animal research
a. Immunizations
b. Chemotherapy
c. Insulin
d. Surgical advances-transplants, cardiac bypass, etc
physical restraint
manual or mechanical means to limit some or all of an animal's normal movement for
the purpose
of examination, collection of samples, drug administration, therapy, or experimental
manipulation.
IACUC
Institutional Animal Care and Use Committee- responsible for all aspects of animal use,
education, health, and compliance with all laws and regulations
areas of biomedical research facility
a. Animal rooms
b. Surgical suite
c. Cage-wash
d. Offices
e. Storage
f. Necropsy Laboratory
g. Clinical Laboratory
h. Personnel areas, shower/lounge
primary enclosures
The outer limit an animal can move without assistance from humans- exc. shoebox for a
rodent, fenced-in field for a large animal, glass tank for aquatics.
macro environment
the area around the animal's primary enclosure. It is the room environment
Micro-environment
the animal's cage
complete air changes required in an hour
10-15
Social environment stimulation
a. Physical
b. Visual
c. Auditory
d. Olfactory
materials for cages
a. Stainless steel
b. Aluminum
c. Plastics
, suspended caging
Racks that hold a series of mesh-bottomed cages that slide in and out on a rack of rails.
metabolism cage
used to collect urine and feces in a manner that allows the total volume to be
measured and analyzed.
biosafety levels (least to greatest)
a. Biosafety Level I
b. Biosafety Level II
c. Biosafety Level III
d. Biosafety Level IV
gnotobiology
The study of organisms that are either germ-free or associated only with known or
specified germs.
proper method for removing a rat from cage
Pick the animal up by the base of the tail and place in next cage or pick it up under its
body. Never pick up by the tip of the tail (can slough or deglove).
malocclusion
improper positioning of the teeth
blood collection techniques from a rat
a. Tail clip
b. Toenail clip
c. Jugular
d. Lateral tail veins
e. Retro-orbital plexus
f. Cardiocentesis
size of a feeding needle for oral gavage in a rat
16-18 gauge, 2-3 inches long
IP
intraperitoneal- administered in the lower left quadrant of the abdomen. The plunger is
withdrawn to verify correct placement. Fluid will enter the hub if it's placed incorrectly. If
no fluid
is present, administration can take place.
ringtail
a condition in a rat that results from low humidity (ideal humidity=50%+ )
Animal Welfare Regulations
sets standards for interstate transportation and export/import of
regulated species
euthanasia
humanely killing animals by methods that induce rapid unconsciousness and death
without pain or distress
Mouse
most common laboratory animal used in research
taxonomic name of the laboratory mouse
Mus musculus
injection techniques used in mice