COMPREHENSIVE EXAM SCRIPT VERIFIED
QUESTIONS ANSWERS ACCURATE
SOLUTIONS PACK
●● Simple staining is used mostly for...
Answer: A quick observance
●● Simple staining uses __________ dye.
Answer: Positively charged
●● Acid fast stain is used for...
Answer: bacteria resistant to traditional staining; mostly for
mycobacterium tuberculosis
●● Resolution
Answer: distance between two objects at which the objects can still be
seen as separate
●● Contrast
Answer: difference in light absorbance between 2 areas
, ●● Dark field microscopy light
Answer: reflects light off of specimen at an angle
●● Can you see intracellular structures with a dark field microscope?
Answer: No
●● What microscope can capture in 3-D?
Answer: Confocal
●● What are the two types of electron microscopes?
Answer: transmission electron microscope (TEM) and scanning electron
microscope (SEM)
●● What is the difference between TEM and SEM?
Answer: TEM passes through the sample, SEM reflects off the surface
●● Gram staining was developed by
Answer: Hans Christian Gram in 1884
●● Gram-positive bacteria shows that
Answer: there is a thick peptidoglycan cell wall