QUESTIONS AND ANSWERS
WITH RATIONALES/GRADED
A+/2026 UPDATE/100% CORRECT
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SECTION A: Cell Biology & Metabolism (Questions 1–20)
1. Which organelle is primarily responsible for ATP synthesis in eukaryotic cells?
• A) Nucleus
• B) Golgi apparatus
• C) Mitochondrion
• D) Ribosome
Rationale: Mitochondria perform oxidative phosphorylation to produce ATP.
Nucleus houses DNA, Golgi modifies proteins, ribosomes synthesize proteins.
2. A plant cell placed in a hypertonic solution will:
• A) Swell and burst
• B) Undergo plasmolysis (shrink away from cell wall)
• C) Remain unchanged due to cell wall
• D) Actively transport water inward
Rationale: Hypertonic solution causes water to leave the cell; the plasma membrane
pulls away from the rigid cell wall (plasmolysis).
3. Which of the following is NOT a stage of cellular respiration?
• A) Glycolysis
• B) Citric acid cycle
• C) Photophosphorylation
, • D) Electron transport chain
Rationale: Photophosphorylation occurs in photosynthesis, not cellular respiration.
4. The fluid mosaic model describes:
• A) The plasma membrane as dynamic with proteins embedded in a lipid
bilayer
• B) DNA structure as a double helix
• C) Enzyme-substrate specificity
• D) Cell wall composition in bacteria
Rationale: Singer and Nicolson’s model emphasizes lateral movement of proteins
and lipids.
5. Which of the following is a catabolic pathway?
• A) Protein synthesis
• B) Glycolysis
• C) DNA replication
• D) Photosynthesis
Rationale: Catabolism breaks down molecules (e.g., glucose to pyruvate) releasing
energy.
6. Where does the Calvin cycle occur in plants?
• A) Thylakoid membrane
• B) Stroma of the chloroplast
• C) Inner mitochondrial membrane
• D) Cytoplasm
Rationale: Calvin cycle (light-independent reactions) fixes CO₂ in the stroma.
7. Which enzyme synthesizes RNA from a DNA template?
• A) DNA polymerase
• B) RNA polymerase
• C) Helicase
• D) Ligase
Rationale: Transcription requires RNA polymerase; DNA polymerase replicates DNA.
8. A cell with 10 chromosomes undergoes mitosis. How many chromosomes will
each daughter cell have?
, • A) 10
• B) 5
• C) 20
• D) 40
Rationale: Mitosis produces genetically identical daughters with the same
chromosome number.
9. Which of the following is an example of active transport?
• A) Oxygen diffusing into a cell
• B) Sodium-potassium pump
• C) Water moving via aquaporins
• D) Glucose facilitated diffusion
Rationale: Active transport requires ATP and moves solutes against gradient (Na⁺
out, K⁺ in).
10. The final electron acceptor in aerobic respiration is:
• A) NAD⁺
• B) FAD
• C) Oxygen
• D) Water
Rationale: O₂ accepts electrons at the end of ETC, forming water.
11. Which organelle contains digestive enzymes that break down damaged
organelles?
• A) Peroxisome
• B) Lysosome
• C) Ribosome
• D) Vacuole
Rationale: Lysosomes have hydrolytic enzymes for autophagy and phagocytosis.
12. In the light reactions of photosynthesis, water is split to provide:
• A) Carbon for sugar
• B) Electrons to photosystem II
• C) ATP directly
• D) NADPH for Calvin cycle only
Rationale: Water oxidation replaces electrons in PSII, releasing O₂ as byproduct.