UAMS-Microbiology Exam 3 Updated Questions
and Answers (2026) | Latest Practice Review
with Detailed Explanations | Grade A+
• Nonfermentors vs enterics . Answer: -Oxidase positive
-OF glucose test
-Colorless growth on MAC
• Nonfermentors . Answer: MO's that cant metabolize sugars in anaerobic conditions
• Fermentor . Answer: Utilize carbohydrates for energy both aerobically and
anaerobically
• Oxidizer . Answer: Metabolizes carbohydrates in ari
• Non-oxidizer . Answer: Cannot utilize sugar as an energy source
• Oxidative-Fermentative Test . Answer: -Determines if a MO is a fermentor, oxidizer or
non-oxidizer
***Make sure to test each MO aerobically and anaerobically***
• Oxidative-Fermentative Test (Nonfermentor, non oxidizer) . Answer: Describe the
reaction for tube set one
• Oxidative-Fermentative Test (Nonfermentor, oxidizer) . Answer: Describe the reaction
for tube set two
• Oxidative-Fermentative Test (Fermentor, oxidizer) . Answer: Describe the reaction for
tube set three
• Nitrate reduction test . Answer: -Determine in MO contains nitrate reductase (convert
nitrate -->nitrite)
-Positive = red
-Negative = no color change
• Nitrate reduction test reagents . Answer: -Sulanilic acid
-Dimethyl-alpha-napthylamine
• If the Nitrate reduction test is negative, add zinc to determine if the MO can convert
nitrate -->nitrite.
-No color change = positive
-Red = negative . Answer: What do you do if the initial nitrate reductase reaction is
negative?
, • Pseudomonas aeruginosa (PSAR) . Answer: -Large, spready colony
-Iridescent sheen
-Beta hemolysis
***Smells like grapes or corn chips***
• Pseudomonas aeruginosa ID tests . Answer: -NLF
-Oxidase +
-Glucose oxidizer(+air, = closed)
-Growth @ 42 degrees
-Nitrate +
-Pyocyanin
• Acinetobacter baumanii . Answer: -GN coccobacilli
-Most common isolate from human specimens
-Most common for HAI**
• Acinetobacter baumanii ID tests . Answer: -Slight pink on MAC
-Oxidase =
-Nitrate =
-OF glucose-saccharolytic (A. baumanii)
-OF glucose-asaccharolytic(A. lowfii)
• Stenotrophomonas maltophilia . Answer: -Aquatic environment
-Oxidae =
-Uses glucose and maltose
-Susceptible to SXT disk
• Burkholderia cepacia . Answer: -Nosocomial infection
-Cepacia syndrome- severe respiratory disease in cystic fibrosis patients
• Burkholderia cepacia media . Answer: BCSA and OFPBL, grows yellow colonies
• Burkholderia pseudomallei . Answer: -Vietnamese time bomb
-Melioidosis
-Bi-polar staining
• Flavobacteria . Answer: -Oxidase +
-yellow/green pigmented colonies
• Vibrio spp . Answer: -Slightly curved GNR
-Halophilic
-Aquatic environment
• Vibrio cholerae . Answer: -Rice water stools
-Darting motility
and Answers (2026) | Latest Practice Review
with Detailed Explanations | Grade A+
• Nonfermentors vs enterics . Answer: -Oxidase positive
-OF glucose test
-Colorless growth on MAC
• Nonfermentors . Answer: MO's that cant metabolize sugars in anaerobic conditions
• Fermentor . Answer: Utilize carbohydrates for energy both aerobically and
anaerobically
• Oxidizer . Answer: Metabolizes carbohydrates in ari
• Non-oxidizer . Answer: Cannot utilize sugar as an energy source
• Oxidative-Fermentative Test . Answer: -Determines if a MO is a fermentor, oxidizer or
non-oxidizer
***Make sure to test each MO aerobically and anaerobically***
• Oxidative-Fermentative Test (Nonfermentor, non oxidizer) . Answer: Describe the
reaction for tube set one
• Oxidative-Fermentative Test (Nonfermentor, oxidizer) . Answer: Describe the reaction
for tube set two
• Oxidative-Fermentative Test (Fermentor, oxidizer) . Answer: Describe the reaction for
tube set three
• Nitrate reduction test . Answer: -Determine in MO contains nitrate reductase (convert
nitrate -->nitrite)
-Positive = red
-Negative = no color change
• Nitrate reduction test reagents . Answer: -Sulanilic acid
-Dimethyl-alpha-napthylamine
• If the Nitrate reduction test is negative, add zinc to determine if the MO can convert
nitrate -->nitrite.
-No color change = positive
-Red = negative . Answer: What do you do if the initial nitrate reductase reaction is
negative?
, • Pseudomonas aeruginosa (PSAR) . Answer: -Large, spready colony
-Iridescent sheen
-Beta hemolysis
***Smells like grapes or corn chips***
• Pseudomonas aeruginosa ID tests . Answer: -NLF
-Oxidase +
-Glucose oxidizer(+air, = closed)
-Growth @ 42 degrees
-Nitrate +
-Pyocyanin
• Acinetobacter baumanii . Answer: -GN coccobacilli
-Most common isolate from human specimens
-Most common for HAI**
• Acinetobacter baumanii ID tests . Answer: -Slight pink on MAC
-Oxidase =
-Nitrate =
-OF glucose-saccharolytic (A. baumanii)
-OF glucose-asaccharolytic(A. lowfii)
• Stenotrophomonas maltophilia . Answer: -Aquatic environment
-Oxidae =
-Uses glucose and maltose
-Susceptible to SXT disk
• Burkholderia cepacia . Answer: -Nosocomial infection
-Cepacia syndrome- severe respiratory disease in cystic fibrosis patients
• Burkholderia cepacia media . Answer: BCSA and OFPBL, grows yellow colonies
• Burkholderia pseudomallei . Answer: -Vietnamese time bomb
-Melioidosis
-Bi-polar staining
• Flavobacteria . Answer: -Oxidase +
-yellow/green pigmented colonies
• Vibrio spp . Answer: -Slightly curved GNR
-Halophilic
-Aquatic environment
• Vibrio cholerae . Answer: -Rice water stools
-Darting motility
