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Voges-Proskauer = No color change
Phenylalanine = No color change
TSI = A/A, H2S; Citrate = Blue
Lysine = Yellow (Control tube - yellow)
Indole = No color change; ONPG = Yellow
Urea = Yellow; Motility = Positive
What is the identification? -✓✓Citrobacter species
Which organism can cause "traveler's diarrhea" in developing countries? -✓✓E. coli
Which organism is typically MUG positive and what does a positive reaction look like? -
✓✓E. coli (not E. coli 0157); fluorescence
Which organisms produce lactose positive colonies on MAC and what is the expected
appearance of the organisms? -✓✓E. coli, Klebsiella & Enterobacter are lactose + &
produce red/pink colonies
Which organism causes most urinary tract infections? -✓✓E. coli
Voges-Proskauer = Pink; Phenylalanine = No color change
KIA = A/A; Citrate = Blue
Lysine = Purple (Control tube - yellow)
Indole = No color change; ONPG = Yellow
Urea = Pink; Motility = Positive
DNAse = No clearing
What is the identification? -✓✓Enterobacter species
Voges-Proskauer = No color change
Phenylalanine = No color change
Kligler iron agar = Yellow/yellow; Citrate = Green
Lysine = Purple (Control tube - yellow); Indole = Red
ONPG = Yellow; Urea = Yellow; Motility = Positive
What is the identification? -✓✓Escherichia coli
Which of the Haemphilus species are NOT normal flora? -✓✓H. ducreyi & H. aegyptius
Which two Haemophilus species require X and V factors for growth? -✓✓H.
haemolyticus (hemolytic) & H. influenzae (nonhemolytic)
, Which two Haemophilus species require V factor for growth? -✓✓H. parahinfluenzae
(nonhemolytic) & H. parahaemolyticus (hemolytic)
Which two Haemophilus species are porphyrin test positive (fluorescence)? -✓✓H.
parainfluenzae (no hemolysis present) & H. parahaemolyticus (hemolysis)
Which organism causes pinkeye? -✓✓Haemophilus aegyptius
Which organism is transmitted sexually and causes a chancroid or soft chancre? -
✓✓Haemophilus ducreyi
Which gram negative bacilli satellite around staphylococci on sheep blood agar? What
nutrient is supplied by the staphylococci? -✓✓Haemophilus; V factor (NAD)
Which two enteric organisms are nonmotile? -✓✓Klebsiella & Shigella
Voges-Proskauer = Pink; Phenylalanine = No color change
TSI = A/A; Citrate = Blue
Lysine = Purple (Control tube - yellow)
Indole = No color change; ONPG = Yellow
Urea = Pink; Motility = Negative
What is the identification? -✓✓Klebsiella pneumoniae
The technologist isolated gram negative diplococci from a sputum specimen. The
following results are obtained:
CHO utilization: glucose =, maltose =, lactose =, sucrose =, nitrate +, DNAse +
What is the identification of the isolate? -✓✓Moraxella catarrhalis
Chromogenic substrate tests were performed on a respiratory isolate. The following
results were obtained:
Hydroxyprolylaminopeptidase =
Gamma-glutamylaminopeptidase =
Beta-glactosidase =
Butyrate esterase +
What is the identification of the organism? -✓✓Moraxella catarrhalis
Voges-Proskauer = No color change; Phenylalanine = Green
TSI = K/A; Citrate = Green
Lysine = Yellow (Control tube - yellow); Indole = Red
ONPG = No color change; Urea = Pink; Motility = Positive
What is the identification? -✓✓Morganella morganii