1|Page
ASCP BOC Qualification in Laboratory Safety (QLS)
Exam ACTUAL VERIFIED EXAM COMPLETE
QUESTIONS AND CORRECT ANSWERS LATEST
UPDATE.pdf
Which factor will not interfere with the reagent strip test for
leukocytes in urine? - Answer-Nitrite. Nitrite is produced by
bacteria, and WBCs are found when there is a bacterial
infection. If nitrite interfered with the WBC reaction, then
the dipstick would be worthless when testing patients with
UTIs.
Which locus is used to identify the gender of the individual
from whom a DNA sample is obtained? - Answer-
Amelogenin. Amelogenin gives different sized amplicons
based on whether it is amplified from the X or Y
chromosomes, so males would have 2 bands on a gel at
the amelogenin locus, while females would have only 1.
Penicillium species is isolated from a bone marrow culture
of a patient that travelled to southeast Asia. After 7 days
the isolate produces a red pigment that diffuses into the
medium. The technologist should: - Answer-Transfer a
colony to BHI at 35 degrees. C. Penicillium marneffiei is a
dimorphic fungi that produces a diffusible red pigment.
,2|Page
An elevated serum iron with normal iron binding capacity
is most likely associated with: - Answer-Pernicious
anemia. Ineffective erythropoiesis. High iron turnover.
Which enzyme substrate is the most specific for prostatic
acid phosphatase for quantitative endpoint reactions? -
Answer-Thymolphthalein monophosphate.
Thymolphthalein monophosphate is the most specific
substrate of choice for quantitative endpoint reactions;
however, p-nitrophenylphosphate is the preferred
substrate for continuous monitoring.
The principle of the tablet test for bilirubin in urine or feces
is: - Answer-Chemical coupling of bilirubin with a
diazonium salt to form a purple color.
Ghost red blood cells are seen in urine that is: - Answer-
Alkaline and dilute. RBCs absorb water when in dilute
urine, and are also less preserved in alkaline urine.
Therefore, as the cells swell in the alkaline urine, the cell
membrane allows hemoglobin to leak from the cell,
resulting in the empty cell membrane and the pale
appearance.
,3|Page
In the cold agglutinin test, the tubes containing the serum
and erythrocytes are allowed to stand overnight in the
refrigerator, and the results are read the next morning. If a
disk of the erythrocytes floats up from the bottom of the
tube with only the flick of finger, this is read as a: - Answer-
A 4+ reaction. When one solid agglutinate is present, the
reaction is classified as 4+.
A bone marrow shows foam cells ranging from 20-100 μm
in size, vacuolated cytoplasm containing sphingomyelin
and is faintly PAS+. This cell type is most characteristic of:
- Answer-Niemann-Pick Disease
The presence of leukocytes in urine is known as: -
Answer-Pyuria
In what metabolic disease will urine turn dark brown to
black upon standing? - Answer-Alkaptonuria
First-generation cephalosporins can be adequately
represented by: - Answer-Cephalothin
, 4|Page
According to AABB Standards, Platelets prepared from
Whole Blood shall have at least: - Answer-5.5x10^10
platelets per unit in at least 90% of the units tested
A stool specimen that appears black and tarry should be
tested for the presence of: - Answer-Occult blood
Sodium bicarbonate and sodium citrate are components of
which of the following? - Answer-JEMBEC system. The
JEMBEC system is a transport and inoculation medium
used for direct plating of specimens for N gonorrhoeae.
Chocolate based selective medium is inoculated with the
specimen. This is placed in an impermeable plastic bag
with a bicarbonatesodium citrate pellet that absorbs
moisture to generate a carbon dioxide-rich environment.
A 44-year-old man was admitted to the hospital following a
2-week history of low-grade fever, malaise and anorexia.
Examination of a Giemsa stain revealed many
intraerythrocytic parasites. Further history revealed
frequent camping trips near Martha's Vineyard and
Nantucket Island, but no travel outside the continental
United States. This parasite could easily be confused with:
- Answer-Plasmodium falciparum. The patient's history is
suggestive of Babesia infection. Babesia microti ring forms
ASCP BOC Qualification in Laboratory Safety (QLS)
Exam ACTUAL VERIFIED EXAM COMPLETE
QUESTIONS AND CORRECT ANSWERS LATEST
UPDATE.pdf
Which factor will not interfere with the reagent strip test for
leukocytes in urine? - Answer-Nitrite. Nitrite is produced by
bacteria, and WBCs are found when there is a bacterial
infection. If nitrite interfered with the WBC reaction, then
the dipstick would be worthless when testing patients with
UTIs.
Which locus is used to identify the gender of the individual
from whom a DNA sample is obtained? - Answer-
Amelogenin. Amelogenin gives different sized amplicons
based on whether it is amplified from the X or Y
chromosomes, so males would have 2 bands on a gel at
the amelogenin locus, while females would have only 1.
Penicillium species is isolated from a bone marrow culture
of a patient that travelled to southeast Asia. After 7 days
the isolate produces a red pigment that diffuses into the
medium. The technologist should: - Answer-Transfer a
colony to BHI at 35 degrees. C. Penicillium marneffiei is a
dimorphic fungi that produces a diffusible red pigment.
,2|Page
An elevated serum iron with normal iron binding capacity
is most likely associated with: - Answer-Pernicious
anemia. Ineffective erythropoiesis. High iron turnover.
Which enzyme substrate is the most specific for prostatic
acid phosphatase for quantitative endpoint reactions? -
Answer-Thymolphthalein monophosphate.
Thymolphthalein monophosphate is the most specific
substrate of choice for quantitative endpoint reactions;
however, p-nitrophenylphosphate is the preferred
substrate for continuous monitoring.
The principle of the tablet test for bilirubin in urine or feces
is: - Answer-Chemical coupling of bilirubin with a
diazonium salt to form a purple color.
Ghost red blood cells are seen in urine that is: - Answer-
Alkaline and dilute. RBCs absorb water when in dilute
urine, and are also less preserved in alkaline urine.
Therefore, as the cells swell in the alkaline urine, the cell
membrane allows hemoglobin to leak from the cell,
resulting in the empty cell membrane and the pale
appearance.
,3|Page
In the cold agglutinin test, the tubes containing the serum
and erythrocytes are allowed to stand overnight in the
refrigerator, and the results are read the next morning. If a
disk of the erythrocytes floats up from the bottom of the
tube with only the flick of finger, this is read as a: - Answer-
A 4+ reaction. When one solid agglutinate is present, the
reaction is classified as 4+.
A bone marrow shows foam cells ranging from 20-100 μm
in size, vacuolated cytoplasm containing sphingomyelin
and is faintly PAS+. This cell type is most characteristic of:
- Answer-Niemann-Pick Disease
The presence of leukocytes in urine is known as: -
Answer-Pyuria
In what metabolic disease will urine turn dark brown to
black upon standing? - Answer-Alkaptonuria
First-generation cephalosporins can be adequately
represented by: - Answer-Cephalothin
, 4|Page
According to AABB Standards, Platelets prepared from
Whole Blood shall have at least: - Answer-5.5x10^10
platelets per unit in at least 90% of the units tested
A stool specimen that appears black and tarry should be
tested for the presence of: - Answer-Occult blood
Sodium bicarbonate and sodium citrate are components of
which of the following? - Answer-JEMBEC system. The
JEMBEC system is a transport and inoculation medium
used for direct plating of specimens for N gonorrhoeae.
Chocolate based selective medium is inoculated with the
specimen. This is placed in an impermeable plastic bag
with a bicarbonatesodium citrate pellet that absorbs
moisture to generate a carbon dioxide-rich environment.
A 44-year-old man was admitted to the hospital following a
2-week history of low-grade fever, malaise and anorexia.
Examination of a Giemsa stain revealed many
intraerythrocytic parasites. Further history revealed
frequent camping trips near Martha's Vineyard and
Nantucket Island, but no travel outside the continental
United States. This parasite could easily be confused with:
- Answer-Plasmodium falciparum. The patient's history is
suggestive of Babesia infection. Babesia microti ring forms
