BIO 305 Final Exam UPDATED QUESTIONS
AND CORRECT ANSWERS
To create transgenic mice, DNA is injected into - CORRECT ANSWER a pronucleus of zygote
How can transgenic organisms be used to identify transcriptional enhancers? - CORRECT
ANSWER A reporter gene can be constructed so that its expression is controlled by an enhancer
that may potentially exist within a fragment of genomic DNA
A trait unique to embryonic stem cells that makes them useful for generating knockout animals is that
they - CORRECT ANSWER are totipotent
after exposure to Agrobacterium tumefaciens bacteria containing both a recombinant T-DNA vector
and a helper plasmid, new plants can be grown from single cells in the presence of herbicide. Without
the herbicide, - CORRECT ANSWER both transformed and nontransformed plants would grow
A scientist wants to determine whether a mutant phenotype is due to the loss of gene C or gene D,
which are both deleted in a mutant fly with abnormal eyes. Mutant flies with transgenic gene C have
wild-type eyes, whereas mutant flies with transgenic gene D have abnormal eyes. What can be
concluded from these results? - CORRECT ANSWER Gene C is required for normal eye
development
The Aniridia gene in humans is involved in eye formation. Although eye development is very
different in flies, this gene is highly conserved. What is the Drosophila homolog of Aniridia? -
CORRECT ANSWER eyeless
The presence of a homeodomain in a protein suggests what about its function? - CORRECT
ANSWER It is a transcription factor
Maternal effect mutations can be identified when - CORRECT ANSWER homozygous mutant
females can develop normally, but their offspring exhibit a mutant phenotype
which of the choices give the correct sequence of early embryonic development in Drosophila? -
CORRECT ANSWER Zygote, multinucleate syncytium, syncytial biastoderm, celluar
blastoderm
AND CORRECT ANSWERS
To create transgenic mice, DNA is injected into - CORRECT ANSWER a pronucleus of zygote
How can transgenic organisms be used to identify transcriptional enhancers? - CORRECT
ANSWER A reporter gene can be constructed so that its expression is controlled by an enhancer
that may potentially exist within a fragment of genomic DNA
A trait unique to embryonic stem cells that makes them useful for generating knockout animals is that
they - CORRECT ANSWER are totipotent
after exposure to Agrobacterium tumefaciens bacteria containing both a recombinant T-DNA vector
and a helper plasmid, new plants can be grown from single cells in the presence of herbicide. Without
the herbicide, - CORRECT ANSWER both transformed and nontransformed plants would grow
A scientist wants to determine whether a mutant phenotype is due to the loss of gene C or gene D,
which are both deleted in a mutant fly with abnormal eyes. Mutant flies with transgenic gene C have
wild-type eyes, whereas mutant flies with transgenic gene D have abnormal eyes. What can be
concluded from these results? - CORRECT ANSWER Gene C is required for normal eye
development
The Aniridia gene in humans is involved in eye formation. Although eye development is very
different in flies, this gene is highly conserved. What is the Drosophila homolog of Aniridia? -
CORRECT ANSWER eyeless
The presence of a homeodomain in a protein suggests what about its function? - CORRECT
ANSWER It is a transcription factor
Maternal effect mutations can be identified when - CORRECT ANSWER homozygous mutant
females can develop normally, but their offspring exhibit a mutant phenotype
which of the choices give the correct sequence of early embryonic development in Drosophila? -
CORRECT ANSWER Zygote, multinucleate syncytium, syncytial biastoderm, celluar
blastoderm
