UTA Microbiology Exam 3 Questions and
Answers (2026/2027) | Updated Review |
A+ Verified
• Binary Fission -✓✓Most common form of bacterial reproduction
• 4 Basic Steps -✓✓1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
• Cytokinesis -✓✓Division of cytoplasm
• Z Ring Assembly -✓✓FtsZ assembles Z ring to form divisome
• Divisome -✓✓Cell division apparatus
• Generation Time -✓✓(Doubling Time)-time it takes to double the population
• Lag Phase -✓✓Cells adjust to culture medium; no change in population
• Log Phase -✓✓Binary fission occurs; cell replication > cell death
• Stationary Phase -✓✓Resources become depleted; cell respiration=cell death
, • Death Phase -✓✓Endospores can form; cell replication < cell death
• Close System Cultures -✓✓Have finite resources
• Open System Cultures -✓✓-Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology
• Microscopic Cell Count -✓✓Cells are counted under a microscope using a laser
etched slide
• Fluorescence Staining -✓✓-Cells are counted under a microscope or flow
cytometer
-Red stain binds to damaged cells
• Viable Cell Counts -✓✓-Samples are diluted and grown on solid media
• Countable Range -✓✓30-300 CFU/ml
• Optical Density -✓✓-Measured with spectrophotometer
-Light is passed thru culture and measured on other side
• Steps of Biofilm Formation -✓✓1. Attachment of planktonic cells
2. Cells become sessile
3. Growth & Division on substrate
Answers (2026/2027) | Updated Review |
A+ Verified
• Binary Fission -✓✓Most common form of bacterial reproduction
• 4 Basic Steps -✓✓1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
• Cytokinesis -✓✓Division of cytoplasm
• Z Ring Assembly -✓✓FtsZ assembles Z ring to form divisome
• Divisome -✓✓Cell division apparatus
• Generation Time -✓✓(Doubling Time)-time it takes to double the population
• Lag Phase -✓✓Cells adjust to culture medium; no change in population
• Log Phase -✓✓Binary fission occurs; cell replication > cell death
• Stationary Phase -✓✓Resources become depleted; cell respiration=cell death
, • Death Phase -✓✓Endospores can form; cell replication < cell death
• Close System Cultures -✓✓Have finite resources
• Open System Cultures -✓✓-Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology
• Microscopic Cell Count -✓✓Cells are counted under a microscope using a laser
etched slide
• Fluorescence Staining -✓✓-Cells are counted under a microscope or flow
cytometer
-Red stain binds to damaged cells
• Viable Cell Counts -✓✓-Samples are diluted and grown on solid media
• Countable Range -✓✓30-300 CFU/ml
• Optical Density -✓✓-Measured with spectrophotometer
-Light is passed thru culture and measured on other side
• Steps of Biofilm Formation -✓✓1. Attachment of planktonic cells
2. Cells become sessile
3. Growth & Division on substrate
