OSSF EXAM 1 MASTER 2 Questions And
Correct Answers (Verified Answers)
Fixation, processing, cutting, staining
What are the four steps to making a histological slide?
10% formalin
What are histological samples fixed in?
Parafin
What are histological samples embedded in?
10x
What magnification does the ocular lens add?
Magnification and resolution
What two factors effect how you see a slide?
Short
What size wavelength is better for clear resolution?
Prevents autolysis, immobilizes cell components, firms up structure
What are three reasons histologic samples get fixed?
Time until fixation, fixation volume, fixation time, tissue thickness, tissue
type
What five factors affect fixation?
10 parts formalin 1 part tissue
What is the minimum fixation volume?
Under fixation
,What is more of a concern, under or over fixation?
Shallow cuts (bread loafing)
What is a way to decrease fixation time for large samples?
Exposed to acid
What must be done to bone before fixation?
Blue
Basophilia is what color?
Pink
Acidophilia/eosinophilia is what color?
Negative (Acidic)
What charges are considered basophilic?
Positive (Basic)
What charges are considered acidophilic/eosinophilic?
Hematoxylin
What is a common basophilic histology stain?
Eosin
What is a common eosinophilic histology stain?
Eosin and methylene blue
What dyes are used in romanovsky stains?
DifQuick and Wright stain
What are two examples of romanovsky stains?
Prussian blue
What specialty stain can detect iron?
,Post hemmorhage
When would a prussian blue stain be useful?
Toluidine blue
What specialty stain polymerizes in the presence of highly negatively charged
compounds, shifting from blue to purple?
Mast cells
What cell type is it useful to stain with toluidine blue?
PAS
What specialty stain produces aldehydes from carbohydrates using periodic
acid and stains them with schiff reagent?
Staining carbohydrates or glycogen
When is PAS useful?
Silver stain
What stain imparts a black color to collagen?
Connective tissue to see collagen
What tissue type would silver stain be used?
Trichrome stain
What specialty stain makes collagen fibers blue or green?
Connective tissue to see collagen
When would a trichrome stain be useful?
Acid fast
What stain is used to see cryptosporidium and mycobacterium?
Macrophage
What cell type would be stained using acid fast?
, Magenta
What color are mycobacteria and cryptosporidium stained with acid fast?
Antemortem
Suture material, bone fragments, and thermal dehydration are examples of
what type of artifact?
Processing
Changes in cell volume due to fixation such as shrinkage and separation of
layers are examples of what type of artifact?
Air bubbles, tears, wrinkling, folds
What four types of artifacts can come during cutting or transferring the
sample?
Plane of section
What is a very common factor that alters what a sample looks like on a slide
resulting from how it was cut?
Blood smear
What is an example of a time romanovsky stains are used?
Cytology
What is the microscopic study of individual cells?
Improved cellular detail and fast
What are two pros to using cytology?
No architecture
What is a con to using cytology?
Histology
What type of sample can have plane of section issues?
Correct Answers (Verified Answers)
Fixation, processing, cutting, staining
What are the four steps to making a histological slide?
10% formalin
What are histological samples fixed in?
Parafin
What are histological samples embedded in?
10x
What magnification does the ocular lens add?
Magnification and resolution
What two factors effect how you see a slide?
Short
What size wavelength is better for clear resolution?
Prevents autolysis, immobilizes cell components, firms up structure
What are three reasons histologic samples get fixed?
Time until fixation, fixation volume, fixation time, tissue thickness, tissue
type
What five factors affect fixation?
10 parts formalin 1 part tissue
What is the minimum fixation volume?
Under fixation
,What is more of a concern, under or over fixation?
Shallow cuts (bread loafing)
What is a way to decrease fixation time for large samples?
Exposed to acid
What must be done to bone before fixation?
Blue
Basophilia is what color?
Pink
Acidophilia/eosinophilia is what color?
Negative (Acidic)
What charges are considered basophilic?
Positive (Basic)
What charges are considered acidophilic/eosinophilic?
Hematoxylin
What is a common basophilic histology stain?
Eosin
What is a common eosinophilic histology stain?
Eosin and methylene blue
What dyes are used in romanovsky stains?
DifQuick and Wright stain
What are two examples of romanovsky stains?
Prussian blue
What specialty stain can detect iron?
,Post hemmorhage
When would a prussian blue stain be useful?
Toluidine blue
What specialty stain polymerizes in the presence of highly negatively charged
compounds, shifting from blue to purple?
Mast cells
What cell type is it useful to stain with toluidine blue?
PAS
What specialty stain produces aldehydes from carbohydrates using periodic
acid and stains them with schiff reagent?
Staining carbohydrates or glycogen
When is PAS useful?
Silver stain
What stain imparts a black color to collagen?
Connective tissue to see collagen
What tissue type would silver stain be used?
Trichrome stain
What specialty stain makes collagen fibers blue or green?
Connective tissue to see collagen
When would a trichrome stain be useful?
Acid fast
What stain is used to see cryptosporidium and mycobacterium?
Macrophage
What cell type would be stained using acid fast?
, Magenta
What color are mycobacteria and cryptosporidium stained with acid fast?
Antemortem
Suture material, bone fragments, and thermal dehydration are examples of
what type of artifact?
Processing
Changes in cell volume due to fixation such as shrinkage and separation of
layers are examples of what type of artifact?
Air bubbles, tears, wrinkling, folds
What four types of artifacts can come during cutting or transferring the
sample?
Plane of section
What is a very common factor that alters what a sample looks like on a slide
resulting from how it was cut?
Blood smear
What is an example of a time romanovsky stains are used?
Cytology
What is the microscopic study of individual cells?
Improved cellular detail and fast
What are two pros to using cytology?
No architecture
What is a con to using cytology?
Histology
What type of sample can have plane of section issues?
