Cell Exam III- Oxidative Phosphorylation UPDATED ACTUAL
QUESTIONS AND CORRECT Answers
what determines TCA speed concentrations of intermediates; higher the concentration increases rate;
intermediates are catalytic
what TCA regulators and what are they regulated by -pyruvate dehydrogenase- ATP neg reg, NAD+ pos reg, AMP pos reg
-isocitrate dehydrogenase- ADP pos reg
-alpha-keto-glutarate dehydrogenase
-malate dehydrogenase
-all have neg reg with NADH
TCA is which means it is both and amphibolic; catabolic (breaking down glucose); anabolic (building other
biomolecules
what intermediates are to form AAs -aminated
-pyruvate -> alanine
-oxaloacetate -> aspartate
-alpha-ketoglutarate -> glutamate
where are high energy e's from NADH and FADH2 used oxidative phosphorylation
2 stages of oxidative phosphorylation 1. energy from e's used to pump protons across membrane (ETC)
2. energy from proton gradient is harnessed to synthesize ATP (ATP synthase)
where does ox phosphorylation occur in eukaryotes in the double membraned mitochondria
permeability of outer mitochondrial membrane has many porins and channels that make it largely permeable to molecules 5
kDa or smaller
inner mitochondrial membrane permeability less permeable and has transporters that support mitochondrial function
how does the inner membrane maximize surface area forming cristae
where are the proteins involved in oxidative in the inner mitochondrial membrane
phosphorylation
where are protons pumped into the inner membrane space
where is there a high [H+] in between outer and inner membranes
what is produced by ox phos in mitochondria most ATP in aerobic cells
where are mitochondria often found parts of cells that require a large amount of ATP: cardiac muscle contraction
and sperm tail swimming
QUESTIONS AND CORRECT Answers
what determines TCA speed concentrations of intermediates; higher the concentration increases rate;
intermediates are catalytic
what TCA regulators and what are they regulated by -pyruvate dehydrogenase- ATP neg reg, NAD+ pos reg, AMP pos reg
-isocitrate dehydrogenase- ADP pos reg
-alpha-keto-glutarate dehydrogenase
-malate dehydrogenase
-all have neg reg with NADH
TCA is which means it is both and amphibolic; catabolic (breaking down glucose); anabolic (building other
biomolecules
what intermediates are to form AAs -aminated
-pyruvate -> alanine
-oxaloacetate -> aspartate
-alpha-ketoglutarate -> glutamate
where are high energy e's from NADH and FADH2 used oxidative phosphorylation
2 stages of oxidative phosphorylation 1. energy from e's used to pump protons across membrane (ETC)
2. energy from proton gradient is harnessed to synthesize ATP (ATP synthase)
where does ox phosphorylation occur in eukaryotes in the double membraned mitochondria
permeability of outer mitochondrial membrane has many porins and channels that make it largely permeable to molecules 5
kDa or smaller
inner mitochondrial membrane permeability less permeable and has transporters that support mitochondrial function
how does the inner membrane maximize surface area forming cristae
where are the proteins involved in oxidative in the inner mitochondrial membrane
phosphorylation
where are protons pumped into the inner membrane space
where is there a high [H+] in between outer and inner membranes
what is produced by ox phos in mitochondria most ATP in aerobic cells
where are mitochondria often found parts of cells that require a large amount of ATP: cardiac muscle contraction
and sperm tail swimming
