AMT MEDICAL LABORATORY SCIENTIST (MLS)
EXAMINATION (LATEST UPDATE 2026/2027)—
ACTUAL EXAM QUESTIONS WITH VERIFIED
CORRECT ANSWERS | GRADED A+
Save
Terms in this set (210) Hide definitions
Which immunoassay methodology is a. Chemiluminescence
the emission of light by molecules in
an excited state with a limited Chemiluminescence is the emission of light by
amount of heat as the result of a molecules in an excited state with a limited amount
chemical reaction? of heat (luminescence) as the result of a chemical
Question reaction. Chemiluminescent assays are
ultrasensitive and are widely used in automated
a.Chemiluminescence immunoassays and DNA probe assay systems.
b.Immunochromatography Chemiluminescence has excellent sensitivity and
c.Photometry dynamic range. Chemiluminescent labels can be
d.Spectrophotometry attached to an antigen or an antibody. Most
chemiluminescent reagents and conjugates are
stable and relatively nontoxic.
,What characteristics/functions do a. Contains a known amount of analyte being
calibrators have? tested
Question
Calibrators are used to establish a correlation
a.Contains a known amount of between the measurement response and the
analyte being tested concentration or amount of the substance that is
b.Monitors the quality of reagents being measured by the test procedure.
c.Monitors the quality of the sample
d.Prevents equipment failure
Absorbance can be calculated easily a. 2 - log10 %T
from percent transmission using
which calculation? Absorbance can be calculated easily from percent
transmission using 2 - log10 %T. If all the light
a.2 - log10 %T passes through a solution without any absorption,
b.2 + log10 %T absorbance is zero and the %T is 100%. If all the
c.log10 %T - 2 light is absorbed, %T is zero, and absorption is
d.log10 %T + 2 infinite.
What is a substance or constituent in c. An analyte
which the laboratory conducts
testing? In the testing process, analytes or constituents are
measured.
a.A solution
b.An additive
c.An analyte
d.An anticoagulant
,What does a common application of d. study crystals in urine and cells
the polarized light microscope in the
clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from
lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells
What is the chelation of calcium in b. Prevents coagulation by inhibiting coagulation
the EDTA tube?
CLSI recommends the salts of the chelating
a.Maintains a constant temperature (calcium binding) agent EDTA as the anticoagulant
for components of choice for blood cell counting and sizing.
b.Prevents coagulation by inhibiting
coagulation
c.Prevents the separation of blood
components
d.Stops the lysing of the red blood
cells
How must a laboratory operate to a. Biosafety level of 2+ or 3
handle TB sputum and TM materials?
BSL-3 builds upon the requirements of BSL-2.
a.Biosafety level of 2+ or 3 Organisms that can cause serious or potentially
b.Biosafety level 4 lethal disease through respiratory transmission are
c.Location where air exchange is typically worked with in a BSL-3 laboratory.
continuous Example: Mycobacterium tuberculosis.
d.Location where fume hood
exhausts are monitored daily
, What are Class A fire extinguishers Ordinary combustibles.
used for?
Class A fire extinguishers are classified as ordinary
a.Electric equipment combustible. Additionally, Class A fire extinguishers
b.Flammable liquids have a numerical rating based on UL-conducted
c.Ordinary combustibles tests and designed to determine the extinguishing
d.Powdered metal (combustible) potential for each size and type of extinguisher
material
Which part of the microscope directs Condenser
and focuses the beam of light from
the bulb onto the material under The condenser is the substage lens of the
examination? microscope that focuses light on the specimen
a.Aperture slide
b.C- mount
c.Condenser
d.Diaphragm
How many milliliters can a 3% Correct Answer: 200 mL
solution make if 6 grams of solute
are available? 3% of solution means 3 g of solute in 100 mL of
solution.
a.100 mL 1 g of solute contains = (100/3) mL
b.200 mL
c.300 mL So, in 6 g of solute = (100/3) x 6 =200 mL.
d.400 mL 200 mL of 3% solution is needed for 6 g of solute.
EXAMINATION (LATEST UPDATE 2026/2027)—
ACTUAL EXAM QUESTIONS WITH VERIFIED
CORRECT ANSWERS | GRADED A+
Save
Terms in this set (210) Hide definitions
Which immunoassay methodology is a. Chemiluminescence
the emission of light by molecules in
an excited state with a limited Chemiluminescence is the emission of light by
amount of heat as the result of a molecules in an excited state with a limited amount
chemical reaction? of heat (luminescence) as the result of a chemical
Question reaction. Chemiluminescent assays are
ultrasensitive and are widely used in automated
a.Chemiluminescence immunoassays and DNA probe assay systems.
b.Immunochromatography Chemiluminescence has excellent sensitivity and
c.Photometry dynamic range. Chemiluminescent labels can be
d.Spectrophotometry attached to an antigen or an antibody. Most
chemiluminescent reagents and conjugates are
stable and relatively nontoxic.
,What characteristics/functions do a. Contains a known amount of analyte being
calibrators have? tested
Question
Calibrators are used to establish a correlation
a.Contains a known amount of between the measurement response and the
analyte being tested concentration or amount of the substance that is
b.Monitors the quality of reagents being measured by the test procedure.
c.Monitors the quality of the sample
d.Prevents equipment failure
Absorbance can be calculated easily a. 2 - log10 %T
from percent transmission using
which calculation? Absorbance can be calculated easily from percent
transmission using 2 - log10 %T. If all the light
a.2 - log10 %T passes through a solution without any absorption,
b.2 + log10 %T absorbance is zero and the %T is 100%. If all the
c.log10 %T - 2 light is absorbed, %T is zero, and absorption is
d.log10 %T + 2 infinite.
What is a substance or constituent in c. An analyte
which the laboratory conducts
testing? In the testing process, analytes or constituents are
measured.
a.A solution
b.An additive
c.An analyte
d.An anticoagulant
,What does a common application of d. study crystals in urine and cells
the polarized light microscope in the
clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from
lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells
What is the chelation of calcium in b. Prevents coagulation by inhibiting coagulation
the EDTA tube?
CLSI recommends the salts of the chelating
a.Maintains a constant temperature (calcium binding) agent EDTA as the anticoagulant
for components of choice for blood cell counting and sizing.
b.Prevents coagulation by inhibiting
coagulation
c.Prevents the separation of blood
components
d.Stops the lysing of the red blood
cells
How must a laboratory operate to a. Biosafety level of 2+ or 3
handle TB sputum and TM materials?
BSL-3 builds upon the requirements of BSL-2.
a.Biosafety level of 2+ or 3 Organisms that can cause serious or potentially
b.Biosafety level 4 lethal disease through respiratory transmission are
c.Location where air exchange is typically worked with in a BSL-3 laboratory.
continuous Example: Mycobacterium tuberculosis.
d.Location where fume hood
exhausts are monitored daily
, What are Class A fire extinguishers Ordinary combustibles.
used for?
Class A fire extinguishers are classified as ordinary
a.Electric equipment combustible. Additionally, Class A fire extinguishers
b.Flammable liquids have a numerical rating based on UL-conducted
c.Ordinary combustibles tests and designed to determine the extinguishing
d.Powdered metal (combustible) potential for each size and type of extinguisher
material
Which part of the microscope directs Condenser
and focuses the beam of light from
the bulb onto the material under The condenser is the substage lens of the
examination? microscope that focuses light on the specimen
a.Aperture slide
b.C- mount
c.Condenser
d.Diaphragm
How many milliliters can a 3% Correct Answer: 200 mL
solution make if 6 grams of solute
are available? 3% of solution means 3 g of solute in 100 mL of
solution.
a.100 mL 1 g of solute contains = (100/3) mL
b.200 mL
c.300 mL So, in 6 g of solute = (100/3) x 6 =200 mL.
d.400 mL 200 mL of 3% solution is needed for 6 g of solute.
