BIOCHEMICAL TECHNIQUES REVISION QUESTIONS
1. Define biochemical analysis and explain its importance in biological research and diagnostics.
2. Differentiate between accuracy and precision in laboratory measurements. Give one example for
each.
3. Explain the terms sensitivity and specificity in biochemical assays.
4. A glucose assay repeatedly gives values close to one another but far from the true value. Is the
assay accurate, precise, both, or neither? Explain.
5. Explain the purpose of calibration in laboratory instruments.
6. Distinguish between calibration and standardization.
7. State five principles of Good Laboratory Practice (GLP).
8. Describe four laboratory safety precautions that should be observed while handling biological
samples.
9. Explain why proper sample collection and handling are important in biochemical analysis.
10. Describe the steps involved in preparing a laboratory reagent solution from a solid chemical.
11. Define a buffer solution and explain how buffers resist changes in pH.
12. State the Henderson–Hasselbalch equation and explain the meaning of each term.
Inline reminder of the equation:
pH=pKa+log([HA][A−])
13. Calculate the pH of a buffer prepared from acetic acid with pKa = 4.76 where [A⁻] = 0.20 M
and [HA] = 0.10 M.
14. Define ionic strength and explain its effect on biochemical reactions.
15. Distinguish between molarity, molality, and normality.
16. Explain how you would prepare 500 mL of 0.5 M NaCl solution.
17. Calculate the volume needed to prepare 100 mL of 1 M HCl from a 5 M stock solution.
18. Explain the difference between stock solutions and working solutions.
19. State the principle of centrifugation and explain how particles separate during centrifugation.
20. Differentiate between differential centrifugation and density gradient centrifugation.
21. Define Relative Centrifugal Force (RCF) and explain its importance in centrifugation
experiments.
22. Differentiate between sedimentation rate and sedimentation coefficient.
23. Explain how particle size, density, and medium viscosity affect sedimentation during
centrifugation.
1. Define biochemical analysis and explain its importance in biological research and diagnostics.
2. Differentiate between accuracy and precision in laboratory measurements. Give one example for
each.
3. Explain the terms sensitivity and specificity in biochemical assays.
4. A glucose assay repeatedly gives values close to one another but far from the true value. Is the
assay accurate, precise, both, or neither? Explain.
5. Explain the purpose of calibration in laboratory instruments.
6. Distinguish between calibration and standardization.
7. State five principles of Good Laboratory Practice (GLP).
8. Describe four laboratory safety precautions that should be observed while handling biological
samples.
9. Explain why proper sample collection and handling are important in biochemical analysis.
10. Describe the steps involved in preparing a laboratory reagent solution from a solid chemical.
11. Define a buffer solution and explain how buffers resist changes in pH.
12. State the Henderson–Hasselbalch equation and explain the meaning of each term.
Inline reminder of the equation:
pH=pKa+log([HA][A−])
13. Calculate the pH of a buffer prepared from acetic acid with pKa = 4.76 where [A⁻] = 0.20 M
and [HA] = 0.10 M.
14. Define ionic strength and explain its effect on biochemical reactions.
15. Distinguish between molarity, molality, and normality.
16. Explain how you would prepare 500 mL of 0.5 M NaCl solution.
17. Calculate the volume needed to prepare 100 mL of 1 M HCl from a 5 M stock solution.
18. Explain the difference between stock solutions and working solutions.
19. State the principle of centrifugation and explain how particles separate during centrifugation.
20. Differentiate between differential centrifugation and density gradient centrifugation.
21. Define Relative Centrifugal Force (RCF) and explain its importance in centrifugation
experiments.
22. Differentiate between sedimentation rate and sedimentation coefficient.
23. Explain how particle size, density, and medium viscosity affect sedimentation during
centrifugation.
