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Which of the following best describes the Central Dogma
of molecular biology?
A. DNA → RNA → Protein
B. Protein → RNA → DNA
C. RNA → DNA → Protein
D. DNA → Protein → RNA
Correct Answer: A
Rationale: The Central Dogma describes the flow of
genetic information from DNA to RNA via transcription,
and from RNA to protein via translation.
A DNA sequence is 5’-ATGGCCTAA-3’. What is the
complementary strand sequence?
A. 3’-TACCGGATT-5’
B. 5’-TACCGGATT-3’
,C. 3’-ATGGCCTAA-5’
D. 5’-ATGGCCTAA-3’
Correct Answer: A
Rationale: Complementary base pairing: A pairs with T,
and C pairs with G. The strands are antiparallel, so the
complementary strand runs 3’ to 5’.
Which enzyme is responsible for synthesizing RNA from a
DNA template during transcription?
A. DNA polymerase
B. RNA polymerase
C. Helicase
D. Ligase
Correct Answer: B
Rationale: RNA polymerase catalyzes transcription,
synthesizing RNA complementary to a DNA template
strand.
,A mutation that changes one nucleotide in a DNA
sequence but does not change the amino acid specified is
called a:
A. Missense mutation
B. Nonsense mutation
C. Silent mutation
D. Frameshift mutation
Correct Answer: C
Rationale: Silent mutations do not alter the amino acid
sequence due to the degeneracy of the genetic code
(multiple codons encode the same amino acid).
During translation, the anticodon is located on:
A. mRNA
B. tRNA
C. rRNA
D. DNA
Correct Answer: B
, Rationale: tRNA molecules carry anticodons that base-
pair with complementary codons on mRNA during
protein synthesis.
Which of the following is an example of a point mutation
that results in a premature stop codon?
A. Missense
B. Nonsense
C. Silent
D. Insertion
Correct Answer: B
Rationale: Nonsense mutations introduce a premature
stop codon (UAA, UAG, UGA), leading to truncated
protein.
The process by which mRNA is decoded to produce a
polypeptide chain is called:
A. Transcription
B. Replication
C. Translation