TIETZ FUNDAMENTALS OF CLINICAL
CHEMISTRY AND MOLECULAR DIAGNOSTICS
149 CERTIFICATION ASSESSMENT 2026
VERIFIED ANSWERS GRADED A+
◉ 2. Which of the following isolates light within a narrow region of
the spectrum?
A. Photomultiplier tube
B. Monochromator
C. Photovoltaic cell
D. Detector.
Answer: B. Monochromator
◉ 3. Which of the following is not descriptive of a photomultiplier
tube?
A. Emits electrons proportionally to initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received.
Answer: C. Cannot be used with a chopper
,◉ 4. Which of the following is false about a photomultiplier tube?
A. Converts radiant energy (light) to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
D. Is composed of an iron plate and a layer of selenium.
Answer: D. Is composed of an iron plate and a layer of selenium
◉ 5. Which type of photodetector employs a linear arrangement that
allows it to respond to a specific wavelength resulting in complete
UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array.
Answer: D. Photodiode array
◉ 6. When performing spectrophotometer quality assurance checks,
what is the holmium oxide glass filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
D. Wavelength accuracy.
,Answer: D. Wavelength accuracy
◉ 7. In spectrophotometric analysis, what is the purpose of the
reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents.
Answer: D. Correct for color contribution of the
reagents
◉ 8. In regard to bichromatic analysis, which
of the following is false?
A. Absorbance is measured at the spectral absorbance peak for a
blank and the sample using the same wavelength.
B. Eliminates background interferences
C. Sample concentration determined from difference in two
measured absorbances
D. Functions as a reference blank for each sample.
Answer: A. Absorbance is measured at the spectral absorbance peak
for a blank and the sample using the same wavelength.
, ◉ 9. The bandpass of a spectrophotometer is 10 nm. If an
installment is set at 540 nm, the wavelengths that are permitted to
impinge on the sample will be within what wavelength range?
A. 530-540 nm
B. 530-550 nm
C. 535-545 nm
D. 540-550 nm.
Answer: C. 535-545 nm
◉ 10. Which of the following formulas is an expression of the Beer-
Lambert law that is routinely applied to spectrophotometric
analysis?
A. Au X (Cs/As) = Cu
B. Cu X (Cs/As) = Au
C. As X (Cs/Cu) = Au
D. A = 2 - log %T.
Answer: A. Au X (Cs/As) = Cu
◉ 1 1 . In spectrophotometry, which of the following is a
mathematical expression of the relationship between absorbance
and transmittance?
A. A = abc
CHEMISTRY AND MOLECULAR DIAGNOSTICS
149 CERTIFICATION ASSESSMENT 2026
VERIFIED ANSWERS GRADED A+
◉ 2. Which of the following isolates light within a narrow region of
the spectrum?
A. Photomultiplier tube
B. Monochromator
C. Photovoltaic cell
D. Detector.
Answer: B. Monochromator
◉ 3. Which of the following is not descriptive of a photomultiplier
tube?
A. Emits electrons proportionally to initial light absorbed
B. Must be shielded from stray light
C. Cannot be used with a chopper
D. Amplifies the initial signal received.
Answer: C. Cannot be used with a chopper
,◉ 4. Which of the following is false about a photomultiplier tube?
A. Converts radiant energy (light) to electrical energy (current)
B. Amplifies the current significantly
C. Has a very rapid response time
D. Is composed of an iron plate and a layer of selenium.
Answer: D. Is composed of an iron plate and a layer of selenium
◉ 5. Which type of photodetector employs a linear arrangement that
allows it to respond to a specific wavelength resulting in complete
UV/visible spectrum analysis?
A. Photomultiplier tube
B. Phototube
C. Barrier layer cell
D. Photodiode array.
Answer: D. Photodiode array
◉ 6. When performing spectrophotometer quality assurance checks,
what is the holmium oxide glass filter used to assess?
A. Linearity
B. Stray light
C. Absorbance accuracy
D. Wavelength accuracy.
,Answer: D. Wavelength accuracy
◉ 7. In spectrophotometric analysis, what is the purpose of the
reagent blank?
A. Correct for interfering chromogens
B. Correct for lipemia
C. Correct for protein
D. Correct for color contribution of the reagents.
Answer: D. Correct for color contribution of the
reagents
◉ 8. In regard to bichromatic analysis, which
of the following is false?
A. Absorbance is measured at the spectral absorbance peak for a
blank and the sample using the same wavelength.
B. Eliminates background interferences
C. Sample concentration determined from difference in two
measured absorbances
D. Functions as a reference blank for each sample.
Answer: A. Absorbance is measured at the spectral absorbance peak
for a blank and the sample using the same wavelength.
, ◉ 9. The bandpass of a spectrophotometer is 10 nm. If an
installment is set at 540 nm, the wavelengths that are permitted to
impinge on the sample will be within what wavelength range?
A. 530-540 nm
B. 530-550 nm
C. 535-545 nm
D. 540-550 nm.
Answer: C. 535-545 nm
◉ 10. Which of the following formulas is an expression of the Beer-
Lambert law that is routinely applied to spectrophotometric
analysis?
A. Au X (Cs/As) = Cu
B. Cu X (Cs/As) = Au
C. As X (Cs/Cu) = Au
D. A = 2 - log %T.
Answer: A. Au X (Cs/As) = Cu
◉ 1 1 . In spectrophotometry, which of the following is a
mathematical expression of the relationship between absorbance
and transmittance?
A. A = abc
